NEK6_PIG
ID NEK6_PIG Reviewed; 313 AA.
AC A2BD05;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Serine/threonine-protein kinase Nek6;
DE EC=2.7.11.1;
DE AltName: Full=Never in mitosis A-related kinase 6;
DE Short=NimA-related protein kinase 6;
GN Name=NEK6;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Large white X Landrace X Duroc;
RX PubMed=17416745; DOI=10.1101/gr.6085507;
RA Mikawa S., Morozumi T., Shimanuki S., Hayashi T., Uenishi H., Domukai M.,
RA Okumura N., Awata T.;
RT "Fine mapping of a swine quantitative trait locus for number of vertebrae
RT and analysis of an orphan nuclear receptor, germ cell nuclear factor
RT (NR6A1).";
RL Genome Res. 17:586-593(2007).
CC -!- FUNCTION: Protein kinase which plays an important role in mitotic cell
CC cycle progression. Required for chromosome segregation at metaphase-
CC anaphase transition, robust mitotic spindle formation and cytokinesis.
CC Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4,
CC STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic
CC spindle formation. Involved in G2/M phase cell cycle arrest induced by
CC DNA damage. Inhibition of activity results in apoptosis. May contribute
CC to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence
CC (By similarity). Phosphorylates EML4 at 'Ser-144', promoting its
CC dissociation from microtubules during mitosis which is required for
CC efficient chromosome congression (By similarity).
CC {ECO:0000250|UniProtKB:Q9HC98}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- ACTIVITY REGULATION: Binding to NEK9 stimulates its activity by
CC releasing the autoinhibitory function of Tyr-108. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with NEK9, predominantly in mitosis. Interacts with
CC KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts
CC with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1,
CC RAD26L, RBBP6, RPS7, RPS6KB1, STAT3 and TRIP4 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC Nucleus speckle {ECO:0000250}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle pole {ECO:0000250}. Note=Colocalizes with APBB1 at the nuclear
CC speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4,
CC CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB
CC and PHF1 in the centrosome. Localizes to spindle microtubules in
CC metaphase and anaphase and to the midbody during cytokinesis (By
CC similarity). {ECO:0000250}.
CC -!- DOMAIN: Displays an autoinhibited conformation: Tyr-108 side chain
CC points into the active site, interacts with the activation loop, and
CC blocks the alphaC helix. The autoinhibitory conformation is released
CC upon binding with NEK9 (By similarity). {ECO:0000250}.
CC -!- PTM: Autophosphorylated. Phosphorylation at Ser-206 is required for its
CC activation. Phosphorylated upon IR or UV-induced DNA damage.
CC Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-
CC regulates phosphorylation at Thr-210 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. NEK Ser/Thr
CC protein kinase family. NIMA subfamily. {ECO:0000305}.
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DR EMBL; AP009124; BAF45329.1; -; Genomic_DNA.
DR RefSeq; XP_003122211.3; XM_003122163.4.
DR RefSeq; XP_005660481.1; XM_005660424.2.
DR RefSeq; XP_013849310.1; XM_013993856.1.
DR AlphaFoldDB; A2BD05; -.
DR SMR; A2BD05; -.
DR STRING; 9823.ENSSSCP00000005983; -.
DR PaxDb; A2BD05; -.
DR PeptideAtlas; A2BD05; -.
DR Ensembl; ENSSSCT00000058361; ENSSSCP00000052701; ENSSSCG00000005587.
DR Ensembl; ENSSSCT00005057657; ENSSSCP00005035531; ENSSSCG00005036029.
DR Ensembl; ENSSSCT00005057683; ENSSSCP00005035548; ENSSSCG00005036029.
DR Ensembl; ENSSSCT00015052084; ENSSSCP00015020795; ENSSSCG00015038799.
DR Ensembl; ENSSSCT00025045186; ENSSSCP00025019260; ENSSSCG00025033186.
DR Ensembl; ENSSSCT00030063821; ENSSSCP00030029221; ENSSSCG00030045680.
DR Ensembl; ENSSSCT00035103043; ENSSSCP00035044025; ENSSSCG00035075710.
DR Ensembl; ENSSSCT00040004407; ENSSSCP00040001404; ENSSSCG00040003483.
DR Ensembl; ENSSSCT00045056815; ENSSSCP00045039683; ENSSSCG00045033184.
DR Ensembl; ENSSSCT00050059114; ENSSSCP00050025370; ENSSSCG00050043427.
DR Ensembl; ENSSSCT00055056215; ENSSSCP00055044913; ENSSSCG00055028329.
DR Ensembl; ENSSSCT00060106188; ENSSSCP00060046844; ENSSSCG00060077158.
DR Ensembl; ENSSSCT00065030351; ENSSSCP00065012422; ENSSSCG00065022786.
DR Ensembl; ENSSSCT00070033640; ENSSSCP00070028075; ENSSSCG00070017021.
DR Ensembl; ENSSSCT00070033654; ENSSSCP00070028089; ENSSSCG00070017021.
DR Ensembl; ENSSSCT00070033660; ENSSSCP00070028094; ENSSSCG00070017021.
DR GeneID; 100514624; -.
DR KEGG; ssc:100514624; -.
DR CTD; 10783; -.
DR VGNC; VGNC:90678; NEK6.
DR eggNOG; KOG0591; Eukaryota.
DR GeneTree; ENSGT00940000159990; -.
DR HOGENOM; CLU_000288_63_23_1; -.
DR InParanoid; A2BD05; -.
DR OrthoDB; 1290401at2759; -.
DR TreeFam; TF101021; -.
DR Reactome; R-SSC-2980767; Activation of NIMA Kinases NEK9, NEK6, NEK7.
DR Reactome; R-SSC-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-SSC-9648025; EML4 and NUDC in mitotic spindle formation.
DR Proteomes; UP000008227; Chromosome 1.
DR Proteomes; UP000314985; Chromosome 1.
DR Bgee; ENSSSCG00000005587; Expressed in liver and 46 other tissues.
DR ExpressionAtlas; A2BD05; baseline and differential.
DR Genevisible; A2BD05; SS.
DR GO; GO:0034451; C:centriolar satellite; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IEA:Ensembl.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IEA:Ensembl.
DR GO; GO:0019894; F:kinesin binding; IEA:Ensembl.
DR GO; GO:0000287; F:magnesium ion binding; IEA:Ensembl.
DR GO; GO:0019901; F:protein kinase binding; ISS:UniProtKB.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:UniProtKB.
DR GO; GO:0001222; F:transcription corepressor binding; IEA:Ensembl.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IEA:Ensembl.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0018105; P:peptidyl-serine phosphorylation; IBA:GO_Central.
DR GO; GO:0046777; P:protein autophosphorylation; ISS:UniProtKB.
DR GO; GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR PRINTS; PR00109; TYRKINASE.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW Apoptosis; ATP-binding; Cell cycle; Cell division; Chromosome partition;
KW Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Mitosis;
KW Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..313
FT /note="Serine/threonine-protein kinase Nek6"
FT /id="PRO_0000311178"
FT DOMAIN 45..310
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..44
FT /note="Interaction with ARHGAP33, ANKRA2, CDC42, PRDX3,
FT RAD26L, RBBP6, RPS7 and TRIP4"
FT /evidence="ECO:0000250"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 267..270
FT /note="Interaction with APBB1"
FT /evidence="ECO:0000250"
FT ACT_SITE 172
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 51..59
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 74
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT SITE 108
FT /note="Autoinhibitory"
FT /evidence="ECO:0000250"
FT MOD_RES 37
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9HC98"
FT MOD_RES 202
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9HC98"
FT MOD_RES 206
FT /note="Phosphoserine; by NEK9"
FT /evidence="ECO:0000250|UniProtKB:Q9HC98"
FT MOD_RES 210
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9HC98"
SQ SEQUENCE 313 AA; 35715 MW; 9EC4EF2B59940DCB CRC64;
MAGQPSHMPH GGSPNNLCHT PGPAHPPDPQ RHPNALSFRC SLADFQIEKK IGRGQFSEVY
KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL NHPNIIKYLD SFIEDNELNI
VLELADAGDL SQMIKYFKKQ KRLIPERTVW KYFVQLCSAV EHMHSRRVMH RDIKPANVFI
TATGIVKLGD LGLGRFFSSE TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM
AALQSPFYGD KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCIYPDP NQRPDIGYVH
QVARQMHVWT SST
