NELFB_DROME
ID NELFB_DROME Reviewed; 594 AA.
AC Q9Y113;
DT 14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Negative elongation factor B;
GN Name=NELF-B; ORFNames=CG32721;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [4]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=15741180; DOI=10.1093/nar/gki274;
RA Wu C.-H., Lee C., Fan R., Smith M.J., Yamaguchi Y., Handa H., Gilmour D.S.;
RT "Molecular characterization of Drosophila NELF.";
RL Nucleic Acids Res. 33:1269-1279(2005).
CC -!- FUNCTION: Essential component of the NELF complex, a complex that
CC negatively regulates the elongation of transcription by RNA polymerase
CC II. {ECO:0000269|PubMed:15741180}.
CC -!- SUBUNIT: Component of the NELF complex. {ECO:0000269|PubMed:15741180}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15741180}. Chromosome
CC {ECO:0000269|PubMed:15741180}. Note=Associates with polytene
CC chromosomes. Associates with the hsp70 promoter when it is inactive,
CC but not when it is activated.
CC -!- SIMILARITY: Belongs to the NELF-B family. {ECO:0000305}.
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DR EMBL; AE014298; AAF46268.1; -; Genomic_DNA.
DR EMBL; AF145659; AAD38634.1; -; mRNA.
DR RefSeq; NP_572402.1; NM_132174.3.
DR AlphaFoldDB; Q9Y113; -.
DR SMR; Q9Y113; -.
DR BioGRID; 58156; 3.
DR DIP; DIP-59537N; -.
DR IntAct; Q9Y113; 3.
DR STRING; 7227.FBpp0071034; -.
DR PaxDb; Q9Y113; -.
DR PRIDE; Q9Y113; -.
DR DNASU; 31681; -.
DR EnsemblMetazoa; FBtr0071077; FBpp0071034; FBgn0027553.
DR GeneID; 31681; -.
DR KEGG; dme:Dmel_CG32721; -.
DR UCSC; CG32721-RA; d. melanogaster.
DR CTD; 31681; -.
DR FlyBase; FBgn0027553; NELF-B.
DR VEuPathDB; VectorBase:FBgn0027553; -.
DR eggNOG; ENOG502QTMJ; Eukaryota.
DR GeneTree; ENSGT00390000012665; -.
DR HOGENOM; CLU_037919_0_0_1; -.
DR InParanoid; Q9Y113; -.
DR OMA; HINEMGQ; -.
DR OrthoDB; 1095614at2759; -.
DR PhylomeDB; Q9Y113; -.
DR Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-DME-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
DR BioGRID-ORCS; 31681; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31681; -.
DR PRO; PR:Q9Y113; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0027553; Expressed in oocyte and 21 other tissues.
DR Genevisible; Q9Y113; DM.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0032021; C:NELF complex; IPI:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0017053; C:transcription repressor complex; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IDA:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR InterPro; IPR010405; COBRA1.
DR PANTHER; PTHR13503; PTHR13503; 1.
DR Pfam; PF06209; COBRA1; 1.
PE 1: Evidence at protein level;
KW Chromosome; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..594
FT /note="Negative elongation factor B"
FT /id="PRO_0000219131"
SQ SEQUENCE 594 AA; 67935 MW; D5A87283888A97F4 CRC64;
MIMSTPAKNN NGTGLEDVNI PGQAYLREAL TSCTDPLKAI ESFQLENGVL LPSLRPMLPL
LDLHGVRRLD FHTSLMEELR DKLIAHINEM GQKEPRERDK KLKELLVKSF PVVRVKSLRP
VVMAILRNTQ HIDDKYLKIL VRDRELYADT DTEVKRQIWR DNQSLFGDEV SPLLSQYIRE
KEHILFDHTN LNNLFFHPTP KVRRQGEVVQ KLANMIGTSV KLYDMVLQFL RTLFLRTRNV
HYCTLRAELL MALHDLEVQE IISIDPCHKF TWCLDACIRE KNVDIKRSRE LQGFLDNIKR
GQEQVLGDLS MTLCDPYAIN FLATSAIKIL HHLINNEGMP RDNQILILLL RMLALGLSAW
VMIDSQDFKE PKLDCQVVTK FLPALMSLMV DDQCRSLHAK LPPDERESAL TTIEHSGPAP
DAVEAYIQES SVASILAMYY TLHTARLKDR VGVLRVLAIL SACKDDRAYE DPFLHSLIAL
LIPMSEEFAT EDFCTTLFDE FIFAGLTREN VTSRHMLKLL WYVHNKLPAG RLATLMKAMQ
PTTAHNEHIH KLYEILQERI GTGAAETPVI EAPPMEFDSP LKSVPTPGPH YNVQ
