NELFD_MOUSE
ID NELFD_MOUSE Reviewed; 591 AA.
AC Q922L6; A2AQ05; Q9QXC8; Q9QXC9; Q9QXD0;
DT 14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Negative elongation factor D;
DE Short=NELF-D;
DE AltName: Full=TH1-like protein;
GN Name=Nelfcd; Synonyms=Nelfd, Th1, Th1l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J, and FVB/N;
RX PubMed=11030415; DOI=10.1007/s004390000344;
RA Bonthron D.T., Hayward B.E., Moran V., Strain L.;
RT "Characterization of TH1 and CTSZ, two non-imprinted genes downstream of
RT GNAS1 in chromosome 20q13.";
RL Hum. Genet. 107:165-175(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic stem cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP INTERACTION WITH KAT8.
RX PubMed=17335777; DOI=10.1016/j.bbrc.2007.02.073;
RA Dmitriev R.I., Korneenko T.V., Bessonov A.A., Shakhparonov M.I.,
RA Modyanov N.N., Pestov N.B.;
RT "Characterization of hampin/MSL1 as a node in the nuclear interactome.";
RL Biochem. Biophys. Res. Commun. 355:1051-1057(2007).
RN [7]
RP INTERACTION WITH NELFB.
RX PubMed=26010750; DOI=10.1371/journal.pone.0127422;
RA Pan H., Zhao X., Zhang X., Abouelsoud M., Sun J., April C., Amleh A.,
RA Fan J.B., Hu Y., Li R.;
RT "Translational initiation at a non-AUG start codon for human and mouse
RT negative elongation factor-B.";
RL PLoS ONE 10:E0127422-E0127422(2015).
CC -!- FUNCTION: Essential component of the NELF complex, a complex that
CC negatively regulates the elongation of transcription by RNA polymerase
CC II (By similarity). The NELF complex, which acts via an association
CC with the DSIF complex and causes transcriptional pausing, is
CC counteracted by the P-TEFb kinase complex (By similarity).
CC {ECO:0000250|UniProtKB:Q8IXH7}.
CC -!- SUBUNIT: The NELF complex is composed of NELFA, NELFB, NELFCD and
CC NELFE; NELFA and NELFCD form a stable subcomplex that binds primarily
CC through NELFCD to the N-terminus of NELFB (By similarity). Binds RNA
CC which may help to stabilize the NELF complex on nucleic acid (By
CC similarity). In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and
CC ssRNA in a sequence- and structure-dependent manner (By similarity).
CC Interacts with ARAF1 (By similarity). Interacts with PCF11 (By
CC similarity). Interacts with NELFB (PubMed:26010750). Interacts with
CC KAT8 (PubMed:17335777). {ECO:0000250|UniProtKB:Q8IXH7,
CC ECO:0000269|PubMed:17335777, ECO:0000269|PubMed:26010750}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8IXH7}.
CC -!- SIMILARITY: Belongs to the NELF-D family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH31747.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB26880.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC33744.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB65252.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB65253.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB65254.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ238741; CAB65252.1; ALT_INIT; mRNA.
DR EMBL; AJ238742; CAB65253.1; ALT_INIT; mRNA.
DR EMBL; AJ238743; CAB65254.1; ALT_INIT; mRNA.
DR EMBL; AK010360; BAB26880.1; ALT_INIT; mRNA.
DR EMBL; AK049418; BAC33744.1; ALT_INIT; mRNA.
DR EMBL; AL844534; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC006959; AAH06959.1; -; mRNA.
DR EMBL; BC031747; AAH31747.1; ALT_INIT; mRNA.
DR CCDS; CCDS38358.1; -.
DR RefSeq; NP_065605.2; NM_020580.3.
DR AlphaFoldDB; Q922L6; -.
DR SMR; Q922L6; -.
DR BioGRID; 208252; 3.
DR STRING; 10090.ENSMUSP00000016397; -.
DR PhosphoSitePlus; Q922L6; -.
DR EPD; Q922L6; -.
DR MaxQB; Q922L6; -.
DR PaxDb; Q922L6; -.
DR PRIDE; Q922L6; -.
DR ProteomicsDB; 252880; -.
DR Antibodypedia; 29232; 182 antibodies from 29 providers.
DR DNASU; 57314; -.
DR Ensembl; ENSMUST00000109075; ENSMUSP00000104703; ENSMUSG00000016253.
DR GeneID; 57314; -.
DR KEGG; mmu:57314; -.
DR UCSC; uc008ofc.1; mouse.
DR CTD; 51497; -.
DR MGI; MGI:1926424; Nelfcd.
DR VEuPathDB; HostDB:ENSMUSG00000016253; -.
DR eggNOG; ENOG502QPUE; Eukaryota.
DR GeneTree; ENSGT00390000001799; -.
DR InParanoid; Q922L6; -.
DR OMA; GFQHEIS; -.
DR OrthoDB; 1288184at2759; -.
DR Reactome; R-MMU-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-MMU-113418; Formation of the Early Elongation Complex.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-MMU-75955; RNA Polymerase II Transcription Elongation.
DR BioGRID-ORCS; 57314; 19 hits in 73 CRISPR screens.
DR ChiTaRS; Nelfcd; mouse.
DR PRO; PR:Q922L6; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q922L6; protein.
DR Bgee; ENSMUSG00000016253; Expressed in embryonic brain and 269 other tissues.
DR ExpressionAtlas; Q922L6; baseline and differential.
DR Genevisible; Q922L6; MM.
DR GO; GO:0032021; C:NELF complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; ISO:MGI.
DR InterPro; IPR006942; TH1.
DR PANTHER; PTHR12144; PTHR12144; 1.
DR Pfam; PF04858; TH1; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Repressor; RNA-binding; Transcription;
KW Transcription regulation.
FT CHAIN 1..591
FT /note="Negative elongation factor D"
FT /id="PRO_0000219132"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 38
FT /note="G -> V (in Ref. 4; AAH06959)"
FT /evidence="ECO:0000305"
FT CONFLICT 416
FT /note="Y -> C (in Ref. 1; CAB65253)"
FT /evidence="ECO:0000305"
FT CONFLICT 479
FT /note="E -> G (in Ref. 1; CAB65254)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 591 AA; 66276 MW; 33DB33547688715D CRC64;
MAGPAPGTIM GEDYFGNASE WGEEADGGQH QEDDSGEGED DAEVQQECLH KFSTRDYIME
PSIFNTLKRY FQAGGSPENV IQLLSENYTA VAQTVNLLAE WLIQTGVEPV QVQETVENHL
KSLLIKHFDP RKADSIFTEE GETPAWLEQM IAHTTWRDLF YKLAEAHPDC LMLNFTVKLI
SDAGYQGEIT SVSTACQQLE VFSRVLRTSL ATILDGGEEN LEKNLPEFAK MVCHGEHTYL
FAQAMMSVLA QEEQGGSAVR RVAQEVQRFA QEKGHDASQI TLALGTAASY PRACQALGAM
LSRGALNPAD ITVLFKMFTS MDPPPVELIR VPAFLDLFMQ SLFKPGAKIN QDHKHKYIHI
LAYAASVVET WKKNKRVSIG KDELKSTSKA IETVHNLCCN ENKGASELVA ELSTLYQCIR
FPVVAMGVLK WVDWTVSEPR YFQLQTDHTP VHLALLDEIS TCHQLLHPQV LQLLVKLFET
EHSQLDVMEQ LELKKTLLDR MVHLLSRGYV LPVVSYIRKC LEKLDTDISL IRYFVTEVLD
VIAPPYTSDF VQLFLPILEN DSIAGTIKAE GEHDPVTEFI AHCKSNFIVV N
