NELFE_DROME
ID NELFE_DROME Reviewed; 280 AA.
AC P92204;
DT 14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Negative elongation factor E;
GN Name=Nelf-E; Synonyms=anon-66Da; ORFNames=CG5994;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9236770; DOI=10.1007/s004380050482;
RA Saeboe-Larssen S., Urbanczyk Mohebi B., Lambertsson A.;
RT "The Drosophila ribosomal protein L14-encoding gene, identified by a novel
RT Minute mutation in a dense cluster of previously undescribed genes in
RT cytogenetic region 66D.";
RL Mol. Gen. Genet. 255:141-151(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH NELF-D.
RX PubMed=12782658; DOI=10.1101/gad.1091403;
RA Wu C.-H., Yamaguchi Y., Benjamin L.R., Horvat-Gordon M., Washinsky J.,
RA Enerly E., Larsson J., Lambertsson A., Handa H., Gilmour D.;
RT "NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in
RT Drosophila.";
RL Genes Dev. 17:1402-1414(2003).
CC -!- FUNCTION: Essential component of the NELF complex, a complex that
CC negatively regulates the elongation of transcription by RNA polymerase
CC II by RNA polymerase II. The NELF complex, which acts via an
CC association with the DSIF complex, causes transcriptional pausing.
CC {ECO:0000269|PubMed:12782658}.
CC -!- SUBUNIT: Component of the NELF complex, which is at least composed of
CC TH1/Nelf-D and Nelf-E. {ECO:0000250}.
CC -!- INTERACTION:
CC P92204; P02828: Hsp83; NbExp=2; IntAct=EBI-194490, EBI-136814;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12782658}. Chromosome
CC {ECO:0000269|PubMed:12782658}. Note=Associates with polytene
CC chromosomes. Associates with the hsp70 promoter when it is inactive,
CC but not when it is activated. The NELF complex possibly dissociates
CC from chromatin following phosphorylation of RNA polymerase II.
CC -!- SIMILARITY: Belongs to the RRM NELF-E family. {ECO:0000305}.
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DR EMBL; Y10015; CAA71120.1; -; Genomic_DNA.
DR EMBL; Y10016; CAA71123.1; -; mRNA.
DR EMBL; AE014296; AAF50394.1; -; Genomic_DNA.
DR EMBL; AY071061; AAL48683.1; -; mRNA.
DR RefSeq; NP_648241.1; NM_139984.3.
DR AlphaFoldDB; P92204; -.
DR SMR; P92204; -.
DR BioGRID; 64390; 19.
DR IntAct; P92204; 6.
DR STRING; 7227.FBpp0076374; -.
DR PaxDb; P92204; -.
DR DNASU; 38982; -.
DR EnsemblMetazoa; FBtr0076650; FBpp0076374; FBgn0017430.
DR GeneID; 38982; -.
DR KEGG; dme:Dmel_CG5994; -.
DR CTD; 38982; -.
DR FlyBase; FBgn0017430; Nelf-E.
DR VEuPathDB; VectorBase:FBgn0017430; -.
DR eggNOG; ENOG502QQQ4; Eukaryota.
DR HOGENOM; CLU_055643_1_0_1; -.
DR InParanoid; P92204; -.
DR OMA; SQKSAHK; -.
DR OrthoDB; 1377312at2759; -.
DR PhylomeDB; P92204; -.
DR Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-DME-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
DR SignaLink; P92204; -.
DR BioGRID-ORCS; 38982; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38982; -.
DR PRO; PR:P92204; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0017430; Expressed in wing disc and 21 other tissues.
DR Genevisible; P92204; DM.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0032021; C:NELF complex; IPI:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0008023; C:transcription elongation factor complex; IPI:UniProtKB.
DR GO; GO:0017053; C:transcription repressor complex; IDA:UniProtKB.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
DR GO; GO:0032785; P:negative regulation of DNA-templated transcription, elongation; IDA:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IDA:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR CDD; cd12305; RRM_NELFE; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR033102; NELFE.
DR InterPro; IPR034637; NELFE_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR PANTHER; PTHR17250; PTHR17250; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Chromosome; Nucleus; Reference proteome; Repressor; RNA-binding;
KW Transcription; Transcription regulation.
FT CHAIN 1..280
FT /note="Negative elongation factor E"
FT /id="PRO_0000081804"
FT DOMAIN 166..236
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 25..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 141..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..94
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 95..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 280 AA; 31811 MW; 9F1F351727D53C47 CRC64;
MVYIHFPNNL TEEEQMLQAK YQKLKKKKKA LQAHKAPKPE PESSLTLKRP TDARDAREVA
RKLIKSGAIP AIQKQTKQDQ TSFKRPKGQE RAKRSTSETT VASYQPFSST QNDVAQETII
SEIIKEEPRR QNLYQHFATE RDREERGMPE KVPMDTAQPE KPRAGNTIFV SGNKVTEDFL
KKTFNDYGTI VNVSMEIEKS RGFVSFAKPE SADRAIAEIH GKNVNGINLQ VQLARRQPQI
EPINDASSSA VWSSIAASKS QKGSHKDHRE MVQYDEDFLL
