ID   NELFE_DROVI             Reviewed;         281 AA.
AC   Q95ZE9;
DT   14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Negative elongation factor E;
GN   Name=Nelf-E; Synonyms=anon-66Da; ORFNames=CG5994;
OS   Drosophila virilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7244;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11814692; DOI=10.1016/s0378-1119(01)00831-9;
RA   Lyamouri M., Enerly E., Kress H., Lambertsson A.;
RT   "Conservation of gene order, structure and sequence between three closely
RT   linked genes in Drosophila melanogaster and Drosophila virilis.";
RL   Gene 282:199-206(2002).
CC   -!- FUNCTION: Essential component of the NELF complex, a complex that
CC       negatively regulates the elongation of transcription by RNA polymerase
CC       II by RNA polymerase II. The NELF complex, which acts via an
CC       association with the DSIF complex, causes transcriptional pausing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NELF complex, which is at least composed of
CC       TH1/NELF-D and NELF-E. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome {ECO:0000250}.
CC       Note=Associates with polytene chromosomes. Associates with the hsp70
CC       promoter when it is inactive, but not when it is activated. The NELF
CC       complex possibly dissociates from chromatin following phosphorylation
CC       of RNA polymerase II (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RRM NELF-E family. {ECO:0000305}.
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DR   EMBL; AJ306692; CAC41628.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q95ZE9; -.
DR   SMR; Q95ZE9; -.
DR   STRING; 7244.FBpp0226729; -.
DR   eggNOG; ENOG502QQQ4; Eukaryota.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0032021; C:NELF complex; IEA:EnsemblMetazoa.
DR   GO; GO:0017053; C:transcription repressor complex; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblMetazoa.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblMetazoa.
DR   CDD; cd12305; RRM_NELFE; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR033102; NELFE.
DR   InterPro; IPR034637; NELFE_RRM.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   PANTHER; PTHR17250; PTHR17250; 2.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SUPFAM; SSF54928; SSF54928; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   3: Inferred from homology;
KW   Chromosome; Nucleus; Repressor; RNA-binding; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..281
FT                   /note="Negative elongation factor E"
FT                   /id="PRO_0000081805"
FT   DOMAIN          167..237
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          25..109
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        41..62
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   281 AA;  31858 MW;  026671F445CFB707 CRC64;
     MVYIHFPNNL TEEEHMLQAK YQKLKKKKKA LQAHKAPKQE PESALTLKRP TDARDAREVA
     RKLIKSGAIP AIQKQQTKQD QTSFKRPKGQ ERAKRSTSET TVAAYQPFSS TQNDVAQETI
     ISEIIKEEPR RQNLYQHFAT ERVREERGLT EKVTLDTTQP EKPRAGNTIF VSGNKVTEEF
     LKKTFNDYGT IVNVSMEIEK SRGFVSFAKP ESADRAIAEM HGKSVTGIVL QVQLARRQPQ
     IVPINDASSS AVWSSIAASK SQKGSHKDLR QMVQYNDDFL M