ID   NEMP1_PONAB             Reviewed;         444 AA.
AC   Q5RDB4;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Nuclear envelope integral membrane protein 1;
DE   Flags: Precursor;
GN   Name=NEMP1; Synonyms=TMEM194A;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBUNIT: Homooligomer. Interacts with RAN-GTP.
CC       {ECO:0000250|UniProtKB:B9X187, ECO:0000250|UniProtKB:Q6ZQE4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC       {ECO:0000250|UniProtKB:Q6ZQE4}; Multi-pass membrane protein
CC       {ECO:0000255}; Nucleoplasmic side {ECO:0000250|UniProtKB:B9X187}.
CC       Nucleus envelope {ECO:0000250|UniProtKB:Q6ZQE4}. Note=Colocalizes with
CC       lamins and RAN-GTP at the nuclear envelope.
CC       {ECO:0000250|UniProtKB:Q6ZQE4}.
CC   -!- DOMAIN: The transmembrane domains are required and sufficient for its
CC       oligomerization. {ECO:0000250|UniProtKB:B9X187}.
CC   -!- PTM: Phosphorylation may regulate its interaction with RAN-GTP.
CC       {ECO:0000250|UniProtKB:Q6ZQE4}.
CC   -!- SIMILARITY: Belongs to the NEMP family. {ECO:0000305}.
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DR   EMBL; CR858000; CAH90243.1; -; mRNA.
DR   RefSeq; NP_001125105.1; NM_001131633.1.
DR   AlphaFoldDB; Q5RDB4; -.
DR   STRING; 9601.ENSPPYP00000005329; -.
DR   GeneID; 100431552; -.
DR   KEGG; pon:100431552; -.
DR   CTD; 23306; -.
DR   eggNOG; KOG3817; Eukaryota.
DR   InParanoid; Q5RDB4; -.
DR   OrthoDB; 536946at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005635; C:nuclear envelope; ISS:UniProtKB.
DR   GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR019358; NEMP_fam.
DR   PANTHER; PTHR13598; PTHR13598; 1.
DR   Pfam; PF10225; NEMP; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Nucleus; Phosphoprotein; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..43
FT                   /evidence="ECO:0000255"
FT   CHAIN           44..444
FT                   /note="Nuclear envelope integral membrane protein 1"
FT                   /id="PRO_0000343660"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        289..309
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          186..297
FT                   /note="A; required for its colocalization with lamins at
FT                   the nuclear envelope"
FT                   /evidence="ECO:0000250|UniProtKB:Q6ZQE4"
FT   REGION          336..444
FT                   /note="Required for nuclear localization"
FT                   /evidence="ECO:0000250|UniProtKB:Q6ZQE4"
FT   REGION          336..405
FT                   /note="B; required for interaction with RAN-GTP"
FT                   /evidence="ECO:0000250|UniProtKB:Q6ZQE4"
FT   MOD_RES         368
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O14524"
FT   MOD_RES         424
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O14524"
FT   MOD_RES         425
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O14524"
FT   CARBOHYD        125
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   444 AA;  50724 MW;  777F73250B8BD3E1 CRC64;
     MAGGMKVAVS PAVGPGPWGS GVGGGGTVRL LLILSGCLVY GTAEIDVNVV MLQESQVCEK
     RASQQFCYTN VLIPKWHDIW TRIQIRVNSS KLVRVTQVEN EQKLKELEQF SIWNFFSSFL
     KEKLNDTYVN VGLYSTKTCL KVEIIEKDTK YSVIVIRRFD PKLFLVFLLG LMLFFCGDLL
     SRSQIFYYST GMSVGIVASL LIIIFILSKF MPKKSPIYVI LVGGWSFSLY LIQLVFKNLQ
     EIWRCYWQYL LSYILTVGFM SFAVCYKYGP LENERSIDLL TWTLQLMGLC FMYSGIQIPH
     IALAIIIIAL CTKNLEYPIQ WLYITYRKVC KAAEKPVPPR LLTEEEYRIQ GEVETRKALE
     ELREFCNSPD CSAWKTVSRI QSPKRFADFV EGSSHLTPNE VSVHEQEYGL GSIIAQDEIY
     EEASSEEEDS YSRCPAITQN NFLT