NEMP2_MOUSE
ID NEMP2_MOUSE Reviewed; 421 AA.
AC Q8CB65; Q8C844;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 3.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Nuclear envelope integral membrane protein 2;
DE Flags: Precursor;
GN Name=Nemp2; Synonyms=Tmem194b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Bone, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000250|UniProtKB:Q6ZQE4}; Multi-pass membrane protein
CC {ECO:0000255}; Nucleoplasmic side {ECO:0000250|UniProtKB:B9X187}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CB65-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CB65-2; Sequence=VSP_033359, VSP_033360;
CC -!- SIMILARITY: Belongs to the NEMP family. {ECO:0000305}.
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DR EMBL; AK036693; BAC29536.1; -; mRNA.
DR EMBL; AK048484; BAC33349.1; -; mRNA.
DR EMBL; AC161876; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS48257.1; -. [Q8CB65-1]
DR RefSeq; NP_001136119.1; NM_001142647.1. [Q8CB65-1]
DR AlphaFoldDB; Q8CB65; -.
DR STRING; 10090.ENSMUSP00000129351; -.
DR PhosphoSitePlus; Q8CB65; -.
DR EPD; Q8CB65; -.
DR MaxQB; Q8CB65; -.
DR PaxDb; Q8CB65; -.
DR PRIDE; Q8CB65; -.
DR ProteomicsDB; 252806; -. [Q8CB65-1]
DR ProteomicsDB; 252807; -. [Q8CB65-2]
DR Antibodypedia; 77970; 4 antibodies from 4 providers.
DR DNASU; 227094; -.
DR Ensembl; ENSMUST00000165859; ENSMUSP00000129351; ENSMUSG00000043015. [Q8CB65-1]
DR Ensembl; ENSMUST00000185483; ENSMUSP00000139513; ENSMUSG00000043015. [Q8CB65-2]
DR GeneID; 227094; -.
DR KEGG; mmu:227094; -.
DR UCSC; uc011wkp.1; mouse. [Q8CB65-1]
DR CTD; 100131211; -.
DR MGI; MGI:2444113; Nemp2.
DR VEuPathDB; HostDB:ENSMUSG00000043015; -.
DR eggNOG; KOG3817; Eukaryota.
DR GeneTree; ENSGT00390000002174; -.
DR HOGENOM; CLU_025225_0_0_1; -.
DR InParanoid; Q8CB65; -.
DR OMA; RYIPKYS; -.
DR OrthoDB; 536946at2759; -.
DR PhylomeDB; Q8CB65; -.
DR TreeFam; TF314831; -.
DR BioGRID-ORCS; 227094; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Nemp2; mouse.
DR PRO; PR:Q8CB65; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8CB65; protein.
DR Bgee; ENSMUSG00000043015; Expressed in ureter smooth muscle and 135 other tissues.
DR ExpressionAtlas; Q8CB65; baseline and differential.
DR Genevisible; Q8CB65; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR019358; NEMP_fam.
DR PANTHER; PTHR13598; PTHR13598; 1.
DR Pfam; PF10225; NEMP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Nucleus; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..421
FT /note="Nuclear envelope integral membrane protein 2"
FT /id="PRO_0000332239"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 204..209
FT /note="YSTFGA -> FMFCAS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_033359"
FT VAR_SEQ 210..421
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_033360"
FT CONFLICT 143
FT /note="S -> G (in Ref. 1; BAC33349)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 47894 MW; 32FD5CAF41557920 CRC64;
MLPRLWWLVL WLQPLATLPA SAVHDEEAAM SVPRCKSLKE TDLIKTSVSD CYCYNQHSQI
QWTYMWSTVQ VTVTSPGLLN IVYITGSHNC QHTESILSFI KCVTHNFWAP EEAEEITIVF
SPYGETVCFS VKPVGRLLPY IVSVSRNIVD FKLFLVFVTG IFLFLYAKTL SQSPVFYYSS
GTVLGILMTL VFVLLMAKKH IPKYSTFGAL MIGCWFASVY VLCQLMEDLK WLWYGNRMYI
LGYVVVVGLC SFAACYSHGP LADEGSRDLL MWTLRLFSLA LVYTGVAAPQ FAYAVLIVLL
FSWSLHYLLR AFSYLRWKMR PWFTAEPQVA RYLTDDEYRE QAEAATARAL EELRQACCRP
DFPSWLAVSR LQAPKKFAEF VLGASHLSPE EVSTHEKQYG LGGAFLEEQL FSLQTDSLPA
S
