NEMU_DROME
ID NEMU_DROME Reviewed; 172 AA.
AC Q8IP68; Q8IGM6;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Protein nemuri {ECO:0000312|EMBL:AAN10843.1};
DE Flags: Precursor;
GN Name=nur {ECO:0000303|PubMed:30705188};
GN ORFNames=CG31813 {ECO:0000312|FlyBase:FBgn0051813};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAN71456.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71456.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAN71456.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=30705188; DOI=10.1126/science.aat1650;
RA Toda H., Williams J.A., Gulledge M., Sehgal A.;
RT "A sleep-inducing gene, nemuri, links sleep and immune function in
RT Drosophila.";
RL Science 363:509-515(2019).
CC -!- FUNCTION: Antimicrobial protein which is essential for the homeostatic
CC regulation of sleep. Promotes sleep following sleep deprivation or
CC bacterial infection and increases survival following bacterial
CC infection. Likely to promote survival to bacterial infection in two
CC ways; by contributing to the innate immune response and by promoting
CC sleep during sickness to aid recovery. {ECO:0000269|PubMed:30705188}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30705188}.
CC -!- TISSUE SPECIFICITY: Detected in the brain where it accumulates in the
CC dorsal fan-shaped body following sleep deprivation (at protein level).
CC Expressed in the adult body. {ECO:0000269|PubMed:30705188}.
CC -!- INDUCTION: In the adult brain by sleep deprivation (at protein level).
CC Up-regulated after bacterial infection in the whole adult body and in
CC brains of some flies. {ECO:0000269|PubMed:30705188}.
CC -!- DISRUPTION PHENOTYPE: Adults display disrupted sleep. Consolidation of
CC daytime sleep is decreased, particularly in males, such that the number
CC of bouts is increased and bout duration is decreased. Adults are more
CC easily roused from sleep and have impaired sleep rebound, thereby
CC recovering sleep more slowly after sleep deprivation. Mutants infected
CC with S.marcescens or E.coli at zeitgeber time 18 (ZT18) show a
CC significant reduction in infection-induced sleep during ZT0 to ZT2 the
CC next morning. {ECO:0000269|PubMed:30705188}.
CC -!- MISCELLANEOUS: The name 'nemuri' means sleep in Japanese.
CC {ECO:0000303|PubMed:30705188}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN71456.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAN10843.1; -; Genomic_DNA.
DR EMBL; AE014134; AHN54420.1; -; Genomic_DNA.
DR EMBL; BT001701; AAN71456.1; ALT_INIT; mRNA.
DR RefSeq; NP_001285906.1; NM_001298977.1.
DR RefSeq; NP_723832.1; NM_165053.2.
DR AlphaFoldDB; Q8IP68; -.
DR IntAct; Q8IP68; 1.
DR STRING; 7227.FBpp0080108; -.
DR PaxDb; Q8IP68; -.
DR DNASU; 318957; -.
DR EnsemblMetazoa; FBtr0080531; FBpp0080108; FBgn0051813.
DR EnsemblMetazoa; FBtr0343656; FBpp0310251; FBgn0051813.
DR GeneID; 318957; -.
DR KEGG; dme:Dmel_CG31813; -.
DR UCSC; CG31813-RA; d. melanogaster.
DR CTD; 318957; -.
DR FlyBase; FBgn0051813; nur.
DR VEuPathDB; VectorBase:FBgn0051813; -.
DR eggNOG; ENOG502TCCG; Eukaryota.
DR HOGENOM; CLU_1556883_0_0_1; -.
DR InParanoid; Q8IP68; -.
DR OMA; NEHRNTA; -.
DR OrthoDB; 1634208at2759; -.
DR PhylomeDB; Q8IP68; -.
DR BioGRID-ORCS; 318957; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 318957; -.
DR PRO; PR:Q8IP68; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0051813; Expressed in cleaving embryo and 10 other tissues.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0019730; P:antimicrobial humoral response; IDA:FlyBase.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0030431; P:sleep; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Coiled coil; Immunity; Innate immunity;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..172
FT /note="Protein nemuri"
FT /evidence="ECO:0000255"
FT /id="PRO_5015099179"
FT REGION 27..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 45..74
FT /evidence="ECO:0000255"
FT COMPBIAS 34..111
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..172
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 172 AA; 19196 MW; 08CAE04DA48391AC CRC64;
MSAKYTLIFA LAALCCLVFS TEAAAQRSRV LSSRRGSELV EKTSDNKEDS ELAAQEQDLE
RQEQEEQNDR LEGRSDDVAE GSDNKEDKET ATNNKDTIVK PNKDDARARR IVRAGRRRGG
RRGGRRGGRR SARKSVRRGG RRGGRRRGGR RGRGGARRRT SVKRRSGKGN KA
