位置:首页 > 蛋白库 > NEN2_ARATH
NEN2_ARATH
ID   NEN2_ARATH              Reviewed;         487 AA.
AC   Q0V842; Q9FLJ5;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Protein NEN2 {ECO:0000303|PubMed:25081480};
DE   AltName: Full=NAC45/NAC86-dependent exonuclease-domain protein 2 {ECO:0000303|PubMed:25081480};
DE            EC=3.1.11.-;
GN   Name=NEN2 {ECO:0000303|PubMed:25081480};
GN   OrderedLocusNames=At5g61390 {ECO:0000312|Araport:AT5G61390};
GN   ORFNames=MFB13.17 {ECO:0000312|EMBL:BAB08494.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|EMBL:ABH04485.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX   PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA   Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT   features of the regions of 1,456,315 bp covered by nineteen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:41-54(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF Clones.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY NAC045
RP   AND NAC086.
RX   PubMed=25081480; DOI=10.1126/science.1253736;
RA   Furuta K.M., Yadav S.R., Lehesranta S., Belevich I., Miyashima S.,
RA   Heo J.O., Vaten A., Lindgren O., De Rybel B., Van Isterdael G.,
RA   Somervuo P., Lichtenberger R., Rocha R., Thitamadee S., Taehtiharju S.,
RA   Auvinen P., Beeckman T., Jokitalo E., Helariutta Y.;
RT   "Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis
RT   culminating in enucleation.";
RL   Science 345:933-937(2014).
CC   -!- FUNCTION: Probable exonuclease involved in enuclation of sieve
CC       elements. {ECO:0000305|PubMed:25081480}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q682U6};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25081480}. Nucleus
CC       {ECO:0000269|PubMed:25081480}. Note=Moves from the cytoplasm to the
CC       nucleus during enuclation. {ECO:0000269|PubMed:25081480}.
CC   -!- TISSUE SPECIFICITY: Expressed in the sieve elements and phloem pole
CC       pericycle cells. {ECO:0000269|PubMed:25081480}.
CC   -!- INDUCTION: Regulated both transcriptionally and post-translationally by
CC       the transcription factors NAC045 and NAC086.
CC       {ECO:0000269|PubMed:25081480}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB08494.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB010073; BAB08494.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED97461.1; -; Genomic_DNA.
DR   EMBL; BT026378; ABH04485.1; -; mRNA.
DR   RefSeq; NP_200947.3; NM_125532.4.
DR   AlphaFoldDB; Q0V842; -.
DR   SMR; Q0V842; -.
DR   STRING; 3702.AT5G61390.1; -.
DR   PaxDb; Q0V842; -.
DR   PRIDE; Q0V842; -.
DR   ProteomicsDB; 251188; -.
DR   EnsemblPlants; AT5G61390.1; AT5G61390.1; AT5G61390.
DR   GeneID; 836260; -.
DR   Gramene; AT5G61390.1; AT5G61390.1; AT5G61390.
DR   KEGG; ath:AT5G61390; -.
DR   Araport; AT5G61390; -.
DR   TAIR; locus:2163208; AT5G61390.
DR   eggNOG; ENOG502QPPQ; Eukaryota.
DR   HOGENOM; CLU_030072_0_0_1; -.
DR   InParanoid; Q0V842; -.
DR   OMA; PYDYQQR; -.
DR   OrthoDB; 567936at2759; -.
DR   PhylomeDB; Q0V842; -.
DR   PRO; PR:Q0V842; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q0V842; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 3.30.420.10; -; 1.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..487
FT                   /note="Protein NEN2"
FT                   /id="PRO_0000430889"
FT   DOMAIN          18..179
FT                   /note="Exonuclease"
FT                   /evidence="ECO:0000255"
FT   REGION          200..233
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          269..291
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        272..288
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        167
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT   BINDING         20
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT   BINDING         20
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT   BINDING         22
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT   BINDING         172
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q91XB0"
SQ   SEQUENCE   487 AA;  54320 MW;  42BAE5234D71C697 CRC64;
     MLTMVGLTPA EDRSEIAFFD VETTIPFRVG QGYAILEFGS ILVCPKKLVE LKNYSVLVRP
     ANLNLITPRS VKCNGIKRED VESALTFADI ADTVYDILHG RIWAGHNILK FDCPRIREAF
     AEIGRDPPEP KGTIDSLALL TQRFGRRAGD MKMATLATYF GLGNQTHRSL DDVRMNFEVL
     KYCATVLFLE SSLPDELIEN SVTTTTPETS SRRRRTIKKS PLQSPTDQQT GENMTTIPIL
     SFVSSAEAQT DPFDMSTLRN EIAPEVLQSD VPMEEEQNQQ SETVASEGTG DQEGFMELDK
     ISVSSIRATH VPLYDGSQTM KLQLFLGDRP LQLHCPRLKV RFGINGKFMD KAGRRRLNFV
     IDLYPSLCNV LQECDSAAQT ISVDSGSGSD WNPLVIPMKG FLNCPTARIH IPTELNGDID
     RYAAEIHQKE FSGATATQKL ISSNPKAEEI ESLLNPRTVL DAFLSLEPYD YQQRAGIRLV
     ARKLVIH
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024