NEN2_ARATH
ID NEN2_ARATH Reviewed; 487 AA.
AC Q0V842; Q9FLJ5;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Protein NEN2 {ECO:0000303|PubMed:25081480};
DE AltName: Full=NAC45/NAC86-dependent exonuclease-domain protein 2 {ECO:0000303|PubMed:25081480};
DE EC=3.1.11.-;
GN Name=NEN2 {ECO:0000303|PubMed:25081480};
GN OrderedLocusNames=At5g61390 {ECO:0000312|Araport:AT5G61390};
GN ORFNames=MFB13.17 {ECO:0000312|EMBL:BAB08494.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABH04485.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY NAC045
RP AND NAC086.
RX PubMed=25081480; DOI=10.1126/science.1253736;
RA Furuta K.M., Yadav S.R., Lehesranta S., Belevich I., Miyashima S.,
RA Heo J.O., Vaten A., Lindgren O., De Rybel B., Van Isterdael G.,
RA Somervuo P., Lichtenberger R., Rocha R., Thitamadee S., Taehtiharju S.,
RA Auvinen P., Beeckman T., Jokitalo E., Helariutta Y.;
RT "Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis
RT culminating in enucleation.";
RL Science 345:933-937(2014).
CC -!- FUNCTION: Probable exonuclease involved in enuclation of sieve
CC elements. {ECO:0000305|PubMed:25081480}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q682U6};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25081480}. Nucleus
CC {ECO:0000269|PubMed:25081480}. Note=Moves from the cytoplasm to the
CC nucleus during enuclation. {ECO:0000269|PubMed:25081480}.
CC -!- TISSUE SPECIFICITY: Expressed in the sieve elements and phloem pole
CC pericycle cells. {ECO:0000269|PubMed:25081480}.
CC -!- INDUCTION: Regulated both transcriptionally and post-translationally by
CC the transcription factors NAC045 and NAC086.
CC {ECO:0000269|PubMed:25081480}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08494.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB010073; BAB08494.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97461.1; -; Genomic_DNA.
DR EMBL; BT026378; ABH04485.1; -; mRNA.
DR RefSeq; NP_200947.3; NM_125532.4.
DR AlphaFoldDB; Q0V842; -.
DR SMR; Q0V842; -.
DR STRING; 3702.AT5G61390.1; -.
DR PaxDb; Q0V842; -.
DR PRIDE; Q0V842; -.
DR ProteomicsDB; 251188; -.
DR EnsemblPlants; AT5G61390.1; AT5G61390.1; AT5G61390.
DR GeneID; 836260; -.
DR Gramene; AT5G61390.1; AT5G61390.1; AT5G61390.
DR KEGG; ath:AT5G61390; -.
DR Araport; AT5G61390; -.
DR TAIR; locus:2163208; AT5G61390.
DR eggNOG; ENOG502QPPQ; Eukaryota.
DR HOGENOM; CLU_030072_0_0_1; -.
DR InParanoid; Q0V842; -.
DR OMA; PYDYQQR; -.
DR OrthoDB; 567936at2759; -.
DR PhylomeDB; Q0V842; -.
DR PRO; PR:Q0V842; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q0V842; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Nucleus; Reference proteome.
FT CHAIN 1..487
FT /note="Protein NEN2"
FT /id="PRO_0000430889"
FT DOMAIN 18..179
FT /note="Exonuclease"
FT /evidence="ECO:0000255"
FT REGION 200..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 269..291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..288
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 167
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT BINDING 20
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT BINDING 20
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT BINDING 22
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q91XB0"
FT BINDING 172
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:Q91XB0"
SQ SEQUENCE 487 AA; 54320 MW; 42BAE5234D71C697 CRC64;
MLTMVGLTPA EDRSEIAFFD VETTIPFRVG QGYAILEFGS ILVCPKKLVE LKNYSVLVRP
ANLNLITPRS VKCNGIKRED VESALTFADI ADTVYDILHG RIWAGHNILK FDCPRIREAF
AEIGRDPPEP KGTIDSLALL TQRFGRRAGD MKMATLATYF GLGNQTHRSL DDVRMNFEVL
KYCATVLFLE SSLPDELIEN SVTTTTPETS SRRRRTIKKS PLQSPTDQQT GENMTTIPIL
SFVSSAEAQT DPFDMSTLRN EIAPEVLQSD VPMEEEQNQQ SETVASEGTG DQEGFMELDK
ISVSSIRATH VPLYDGSQTM KLQLFLGDRP LQLHCPRLKV RFGINGKFMD KAGRRRLNFV
IDLYPSLCNV LQECDSAAQT ISVDSGSGSD WNPLVIPMKG FLNCPTARIH IPTELNGDID
RYAAEIHQKE FSGATATQKL ISSNPKAEEI ESLLNPRTVL DAFLSLEPYD YQQRAGIRLV
ARKLVIH