ID   NEOD_STRFR              Reviewed;         421 AA.
AC   Q53U18; Q2MFE3; Q4W2P5;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=2-deoxystreptamine N-acetyl-D-glucosaminyltransferase;
DE            EC=2.4.1.283;
DE   AltName: Full=Neomycin biosynthesis protein 8;
DE            Short=Neo-8;
DE   AltName: Full=Neomycin biosynthesis protein D;
GN   Name=neoD; Synonyms=nemD, neo8;
OS   Streptomyces fradiae (Streptomyces roseoflavus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RX   PubMed=16506694; DOI=10.1038/ja.2005.104;
RA   Kudo F., Yamamoto Y., Yokoyama K., Eguchi T., Kakinuma K.;
RT   "Biosynthesis of 2-deoxystreptamine by three crucial enzymes in
RT   Streptomyces fradiae NBRC 12773.";
RL   J. Antibiot. 58:766-774(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RX   PubMed=15827636; DOI=10.1039/b501199j;
RA   Huang F., Haydock S.F., Mironenko T., Spiteller D., Li Y., Spencer J.B.;
RT   "The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233:
RT   characterisation of an aminotransferase involved in the formation of 2-
RT   deoxystreptamine.";
RL   Org. Biomol. Chem. 3:1410-1418(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RA   Aboshanab K.M., Schmidt-Beissner H., Wehmeier U.F., Piepersberg W.,
RA   Welzel K., Vente A.;
RT   "Analysis and comparison of biosynthetic gene clusters for the 2-deoxy-
RT   inosamine containing aminoglycoside antibiotics ribostamycin, neomycin,
RT   lividomycin, paromomycin and butirosin.";
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RA   Subba B., Kharel M.K., Sthapit B., Liou K., Lee H.C., Woo J.S., Sohng J.K.;
RT   "Cloning and characterization of a neomycin biosynthetic gene cluster from
RT   Streptomyces fradiae, ATCC 10745.";
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=18311744; DOI=10.1002/cbic.200700717;
RA   Yokoyama K., Yamamoto Y., Kudo F., Eguchi T.;
RT   "Involvement of two distinct N-acetylglucosaminyltransferases and a dual-
RT   function deacetylase in neomycin biosynthesis.";
RL   ChemBioChem 9:865-869(2008).
CC   -!- FUNCTION: Glycosyltransferase involved in the biosynthesis of neomycin
CC       by mediating conversion of 2-deoxystreptamine (2-DOS) to 2'-N-
CC       acetylparomamine using UDP-alpha-D-glucosamine as sugar donor.
CC       {ECO:0000269|PubMed:18311744}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-deoxystreptamine + UDP-N-acetyl-alpha-D-glucosamine = 2'-N-
CC         acetylparomamine + H(+) + UDP; Xref=Rhea:RHEA:33947,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:65010, ChEBI:CHEBI:65069; EC=2.4.1.283;
CC         Evidence={ECO:0000269|PubMed:18311744};
CC   -!- PATHWAY: Antibiotic biosynthesis; neomycin biosynthesis.
CC       {ECO:0000269|PubMed:18311744}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAF33313.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB211959; BAD95821.1; -; Genomic_DNA.
DR   EMBL; AJ843080; CAH58691.1; -; Genomic_DNA.
DR   EMBL; AJ629247; CAF33313.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AJ786317; CAH05100.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q53U18; -.
DR   SMR; Q53U18; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   KEGG; ag:BAD95821; -.
DR   BioCyc; MetaCyc:MON-17260; -.
DR   UniPathway; UPA00969; -.
DR   GO; GO:0102319; F:2-deoxystreptamine N-acetyl-D-glucosaminyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:1901158; P:neomycin biosynthetic process; IDA:UniProtKB.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13579; Glyco_trans_4_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..421
FT                   /note="2-deoxystreptamine N-acetyl-D-
FT                   glucosaminyltransferase"
FT                   /id="PRO_0000421742"
SQ   SEQUENCE   421 AA;  45332 MW;  20A7143DA4B58959 CRC64;
     MRVLRLTPFF HHDCVTAWPA EFDAVGGMQV QILRLSRELA DRGVEQLVMT VGFPGLPRER
     VDRPGLRVRV TRAPLPRLRS ELTGLVGLNQ AWLAAVLTAC APLRRTWRPD LVHVHADGQL
     WALLAGPLVS RLVGAPYCLT LHCSRLASYE PMSRFDRLQH RLVAAAERYA LRRARRVSTL
     TSRTADTVAR LLPLDRALVD VLPDSVGDVR PVARPEAEEY VRSLGVPAGR PVVGWVGRVA
     HEKGWRDFVA MAERWDAGSG APGAVFAVVG DGPQRERMRE AVEAAGLADR FVFTGFLPHD
     AVPSVMTALD VLVMPSAHEE LGGSALEAMV CGTPVAGYAV GGLRDTVGSV TPSLLVPRGD
     VAALTRAAGD AVTDAERHRK TVAAAVPDLL GRYGADTVER ALEHYRLAVG RASGGGAGWA
     P