ID   NEOK_STRFR              Reviewed;         366 AA.
AC   Q53U11; Q4W2N8;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=UDP-GlcNAc:ribostamycin N-acetylglucosaminyltransferase;
DE            EC=2.4.1.285;
DE   AltName: Full=Neomycin biosynthesis protein 15;
DE            Short=Neo-15;
DE   AltName: Full=Neomycin biosynthesis protein K;
GN   Name=neoK; Synonyms=neo15, neoF;
OS   Streptomyces fradiae (Streptomyces roseoflavus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RX   PubMed=16506694; DOI=10.1038/ja.2005.104;
RA   Kudo F., Yamamoto Y., Yokoyama K., Eguchi T., Kakinuma K.;
RT   "Biosynthesis of 2-deoxystreptamine by three crucial enzymes in
RT   Streptomyces fradiae NBRC 12773.";
RL   J. Antibiot. 58:766-774(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RX   PubMed=15827636; DOI=10.1039/b501199j;
RA   Huang F., Haydock S.F., Mironenko T., Spiteller D., Li Y., Spencer J.B.;
RT   "The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233:
RT   characterisation of an aminotransferase involved in the formation of 2-
RT   deoxystreptamine.";
RL   Org. Biomol. Chem. 3:1410-1418(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC   8233 / NRRL B-1195 / VKM Ac-150;
RA   Aboshanab K.M., Schmidt-Beissner H., Wehmeier U.F., Piepersberg W.,
RA   Welzel K., Vente A.;
RT   "Analysis and comparison of biosynthetic gene clusters for the 2-deoxy-
RT   inosamine containing aminoglycoside antibiotics ribostamycin, neomycin,
RT   lividomycin, paromomycin and butirosin.";
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND PATHWAY.
RX   PubMed=18311744; DOI=10.1002/cbic.200700717;
RA   Yokoyama K., Yamamoto Y., Kudo F., Eguchi T.;
RT   "Involvement of two distinct N-acetylglucosaminyltransferases and a dual-
RT   function deacetylase in neomycin biosynthesis.";
RL   ChemBioChem 9:865-869(2008).
CC   -!- FUNCTION: Glycosyltransferase involved in the biosynthesis of neomycin
CC       by mediating glycosylation of ribostamycin with UDP-GlcNAc as a sugar
CC       donor to generate 2'''-acetyl-6'''-hydroxyneomycin C.
CC       {ECO:0000269|PubMed:18311744}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ribostamycin + UDP-N-acetyl-alpha-D-glucosamine = 2'''-acetyl-
CC         6'''-hydroxyneomycin C + H(+) + UDP; Xref=Rhea:RHEA:33951,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:65028, ChEBI:CHEBI:65030; EC=2.4.1.285;
CC         Evidence={ECO:0000269|PubMed:18311744};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000269|PubMed:18311744};
CC   -!- PATHWAY: Antibiotic biosynthesis; neomycin biosynthesis.
CC       {ECO:0000269|PubMed:18311744}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR   EMBL; AB211959; BAD95828.1; -; Genomic_DNA.
DR   EMBL; AJ843080; CAH58698.1; -; Genomic_DNA.
DR   EMBL; AJ629247; CAF33320.1; -; Genomic_DNA.
DR   RefSeq; WP_031132485.1; NZ_MUNC01000172.1.
DR   AlphaFoldDB; Q53U11; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   KEGG; ag:BAD95828; -.
DR   BioCyc; MetaCyc:MON-17261; -.
DR   UniPathway; UPA00969; -.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:1901158; P:neomycin biosynthetic process; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..366
FT                   /note="UDP-GlcNAc:ribostamycin N-
FT                   acetylglucosaminyltransferase"
FT                   /id="PRO_0000421743"
FT   REGION          342..366
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        174
FT                   /note="D -> H (in Ref. 2; CAH58698)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   366 AA;  39257 MW;  C527A6FCA4F253CF CRC64;
     MAEAPAGRAL FEIYDEGFDS PSWGGVETAL WHLSRSLREA GTEAEFYRSS EGADLDALAA
     RVERDRVDAV FPLVESDLFE GAAWRRLPAL HARTVRVWHD VSRLSADLSA PPPCPVHARV
     PALPGAPVAE GCPARGAHPE GPMREVFLGE WPWTRCFPRR SVIPWAADHV PAKDLCDPSG
     PVVLQLGKID TVDAERCLRR LTGAGVALRV VFATWSRRGR EARELVRAHQ GAGRRVEVLD
     AYDIRTDWER VFGGASLFLL PSVFHETYNF AAAEAVQLGV PVAALGEGGN LPRFASLTAP
     TPDALVDRLL AGGGAVAPRP RPAAGWRDVA ARYAEVIREH PAAGAGPAVP AGAGEGRGGR
     EEEHGG