NEOL_STRFR
ID NEOL_STRFR Reviewed; 279 AA.
AC Q53U10; Q4A4C8;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=2'-N-acetylparomamine deacetylase;
DE EC=3.5.1.112;
DE AltName: Full=2'''-acetyl-6'''-hydroxyneomycin C deacetylase;
DE EC=3.5.1.113;
DE AltName: Full=Neomycin biosynthesis protein 16;
DE Short=Neo-16;
DE AltName: Full=Neomycin biosynthesis protein L;
GN Name=neoL; Synonyms=neo16, neoD;
OS Streptomyces fradiae (Streptomyces roseoflavus).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=1906;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC 8233 / NRRL B-1195 / VKM Ac-150;
RX PubMed=16506694; DOI=10.1038/ja.2005.104;
RA Kudo F., Yamamoto Y., Yokoyama K., Eguchi T., Kakinuma K.;
RT "Biosynthesis of 2-deoxystreptamine by three crucial enzymes in
RT Streptomyces fradiae NBRC 12773.";
RL J. Antibiot. 58:766-774(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC 8233 / NRRL B-1195 / VKM Ac-150;
RX PubMed=15827636; DOI=10.1039/b501199j;
RA Huang F., Haydock S.F., Mironenko T., Spiteller D., Li Y., Spencer J.B.;
RT "The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233:
RT characterisation of an aminotransferase involved in the formation of 2-
RT deoxystreptamine.";
RL Org. Biomol. Chem. 3:1410-1418(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC 8233 / NRRL B-1195 / VKM Ac-150;
RA Aboshanab K.M., Schmidt-Beissner H., Wehmeier U.F., Piepersberg W.,
RA Welzel K., Vente A.;
RT "Analysis and comparison of biosynthetic gene clusters for the 2-deoxy-
RT inosamine containing aminoglycoside antibiotics ribostamycin, neomycin,
RT lividomycin, paromomycin and butirosin.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10745 / CBS 498.68 / DSM 40063 / JCM 4133 / NBRC 12773 / NCIMB
RC 8233 / NRRL B-1195 / VKM Ac-150;
RA Subba B., Kharel M.K., Sthapit B., Liou K., Lee H.C., Woo J.S., Sohng J.K.;
RT "Cloning and characterization of a neomycin biosynthetic gene cluster from
RT Streptomyces fradiae, ATCC 10745.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX PubMed=18311744; DOI=10.1002/cbic.200700717;
RA Yokoyama K., Yamamoto Y., Kudo F., Eguchi T.;
RT "Involvement of two distinct N-acetylglucosaminyltransferases and a dual-
RT function deacetylase in neomycin biosynthesis.";
RL ChemBioChem 9:865-869(2008).
CC -!- FUNCTION: Deacetylase involved in the biosynthesis of neomycin by
CC mediating 2 steps of the pathway. Deacetylates both 2'-N-
CC acetylparomamine and 2'''-acetyl-6'''-hydroxyneomycin C.
CC {ECO:0000269|PubMed:18311744}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2'-N-acetylparomamine + H2O = acetate + paromamine;
CC Xref=Rhea:RHEA:34031, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:65010, ChEBI:CHEBI:65015; EC=3.5.1.112;
CC Evidence={ECO:0000269|PubMed:18311744};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2'''-acetyl-6'''-hydroxyneomycin C + H2O = 6'''-deamino-6'''-
CC hydroxyneomycin C + acetate; Xref=Rhea:RHEA:34051, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:30089, ChEBI:CHEBI:65030, ChEBI:CHEBI:65031;
CC EC=3.5.1.113; Evidence={ECO:0000269|PubMed:18311744};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- PATHWAY: Antibiotic biosynthesis; neomycin biosynthesis.
CC {ECO:0000269|PubMed:18311744}.
CC -!- SIMILARITY: Belongs to the PIGL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAH05093.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB211959; BAD95829.1; -; Genomic_DNA.
DR EMBL; AJ843080; CAH58699.1; -; Genomic_DNA.
DR EMBL; AJ629247; CAF33321.1; -; Genomic_DNA.
DR EMBL; AJ786317; CAH05093.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; Q53U10; -.
DR SMR; Q53U10; -.
DR KEGG; ag:BAD95829; -.
DR BioCyc; MetaCyc:MON-17259; -.
DR UniPathway; UPA00969; -.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:1901158; P:neomycin biosynthetic process; IDA:UniProtKB.
DR Gene3D; 3.40.50.10320; -; 1.
DR InterPro; IPR003737; GlcNAc_PI_deacetylase-related.
DR InterPro; IPR024078; LmbE-like_dom_sf.
DR Pfam; PF02585; PIG-L; 1.
DR SUPFAM; SSF102588; SSF102588; 1.
PE 1: Evidence at protein level;
KW Antibiotic biosynthesis; Hydrolase; Metal-binding; Transferase; Zinc.
FT CHAIN 1..279
FT /note="2'-N-acetylparomamine deacetylase"
FT /id="PRO_0000421745"
FT REGION 245..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 31
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 34
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
FT BINDING 157
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250"
SQ SEQUENCE 279 AA; 30109 MW; 9E69F7930B40152A CRC64;
MGEPTWEAAE DPDRTLRERL RRGRTLLVSP HPDDVAYSCG GLLAAVGRPA HATLLTVFTR
SAWALPRRLR RAGARVVSER RREEELRYCR LRGLAEYRPL GFADAGLRGY DDETELSSPA
EADGVRGAVE EAVAEAIRDA GADTVLAPAA VGGHVDHLLV HGAVRGAVGP GGPLTLFYED
LPYAGQRDAV DVERTLREAR GLVPFASVDI SGVVQQKVRG MYVYGSQTDD ECVRETLRHA
RRGAPRRWTG GTAGAGHAAG RRGAPHTERV WTPAPAGAR
