ID   NEP3L_NEMVE             Reviewed;         432 AA.
AC   A7RJ13;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Nematocyst expressed protein 3-like {ECO:0000305};
DE            Short=NEP-3-like {ECO:0000305};
DE            Short=NEP3-like {ECO:0000303|PubMed:29424690};
DE   Flags: Precursor;
GN   ORFNames=v1g238655 {ECO:0000312|EMBL:EDO48497.1};
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1] {ECO:0000312|EMBL:EDO48497.1, ECO:0000312|Proteomes:UP000001593}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CH2 X CH6 {ECO:0000312|Proteomes:UP000001593};
RX   PubMed=17615350; DOI=10.1126/science.1139158;
RA   Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA   Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA   Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA   Technau U., Martindale M.Q., Rokhsar D.S.;
RT   "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT   genomic organization.";
RL   Science 317:86-94(2007).
RN   [2]
RP   DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX   PubMed=29424690; DOI=10.7554/elife.35014;
RA   Columbus-Shenkar Y.Y., Sachkova M.Y., Macrander J., Fridrich A.,
RA   Modepalli V., Reitzel A.M., Sunagar K., Moran Y.;
RT   "Dynamics of venom composition across a complex life cycle.";
RL   Elife 7:0-0(2018).
CC   -!- FUNCTION: Probable toxin. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nematocyst {ECO:0000269|PubMed:29424690}.
CC       Secreted {ECO:0000269|PubMed:29424690}.
CC   -!- TISSUE SPECIFICITY: Nematocytes (PubMed:29424690). In late planulae,
CC       transcripts are found throughout the ectoderm in nematocytes, with high
CC       concentration of expressing cells in the oral pole (PubMed:29424690).
CC       In primary polyps, is expressed in nematocytes in the body wall and
CC       physa ectoderm and in the upper and lower pharynx (PubMed:29424690).
CC       {ECO:0000269|PubMed:29424690}.
CC   -!- DEVELOPMENTAL STAGE: Transcripts are expressed in gastrulae, early and
CC       late planulae, metamorphosing planulae, and primary polyps.
CC       {ECO:0000269|PubMed:29424690}.
CC   -!- SIMILARITY: Belongs to the NEP3 family. {ECO:0000305}.
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DR   EMBL; DS469513; EDO48497.1; -; Genomic_DNA.
DR   RefSeq; XP_001640560.1; XM_001640510.1.
DR   SMR; A7RJ13; -.
DR   STRING; 45351.EDO48497; -.
DR   EnsemblMetazoa; EDO48497; EDO48497; NEMVEDRAFT_v1g238655.
DR   GeneID; 5520740; -.
DR   KEGG; nve:5520740; -.
DR   eggNOG; KOG1217; Eukaryota.
DR   HOGENOM; CLU_635095_0_0_1; -.
DR   OMA; CDGGCPC; -.
DR   OrthoDB; 1917747at2759; -.
DR   Proteomes; UP000001593; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR003582; ShKT_dom.
DR   SMART; SM00254; ShKT; 2.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Nematocyst; Reference proteome; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..432
FT                   /note="Nematocyst expressed protein 3-like"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_5002714244"
FT   DOMAIN          59..91
FT                   /note="ShKT 1"
FT                   /evidence="ECO:0000305|PubMed:29424690"
FT   DOMAIN          107..143
FT                   /note="ShKT 2"
FT                   /evidence="ECO:0000305|PubMed:29424690"
FT   DOMAIN          149..183
FT                   /note="ShKT 3"
FT                   /evidence="ECO:0000305|PubMed:29424690"
FT   REGION          235..432
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        304..354
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        382..402
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        411..432
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        52..91
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        59..84
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        73..88
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        125..140
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        157..176
FT                   /evidence="ECO:0000250|UniProtKB:A7RNZ0"
FT   DISULFID        166..180
FT                   /evidence="ECO:0000250|UniProtKB:A7RNZ0"
SQ   SEQUENCE   432 AA;  44698 MW;  93221217C7BDD2BD CRC64;
     MRVVYLVLVV AVIIAITEAK SVKKSKTRSP KHPRHRTLAD DYKLNRISAK YCKDYDDSCK
     AFGKIAKKDK KSCFNNPDKA RMACPVSCKL CDKKTFHKKS KLSDVVVVRA MCVDIEVDQC
     NPDVCYTNPD WATENCRKTC MLCKPDIRGP CDTDPRCPFW GQYGYCSTAT YIDNHCPYNC
     DVYSYVPEAP QPYPLPIEPL YAYQPLPYPT APPGVTPATL PPYYENAPQP TYAPGAQYPA
     ATAAPAPSAP GATAAPAPSA PGATAAPAPS APEATAAPAP SAPEATAAPA PSAPEATAAP
     APAPEAAPSE PEAAPAPAPE MAPAPAPEMA PAPEAASAPA PEAAPAPEAA SAPAPEAAPA
     PEAASAPAPE AASAPAPEAA PAPEAASAPA PEAAPAPEAA PSEQPMPGKK SKSKPSKRKG
     VKKSKSGHKR HH