NEP3_NEMVE
ID NEP3_NEMVE Reviewed; 355 AA.
AC A7RJ12;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 23-FEB-2022, entry version 43.
DE RecName: Full=Nematocyst expressed protein 3 {ECO:0000303|PubMed:23151943};
DE Short=NEP-3 {ECO:0000303|PubMed:23151943};
DE Short=NEP3 {ECO:0000303|PubMed:29424690, ECO:0000303|PubMed:31134275};
DE Flags: Precursor;
GN ORFNames=v1g238654 {ECO:0000312|EMBL:EDO48496.1};
OS Nematostella vectensis (Starlet sea anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Edwardsiidae; Nematostella.
OX NCBI_TaxID=45351;
RN [1] {ECO:0000312|EMBL:EDO48496.1, ECO:0000312|Proteomes:UP000001593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CH2 X CH6 {ECO:0000312|Proteomes:UP000001593};
RX PubMed=17615350; DOI=10.1126/science.1139158;
RA Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA Technau U., Martindale M.Q., Rokhsar D.S.;
RT "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT genomic organization.";
RL Science 317:86-94(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23151943; DOI=10.1007/s10126-012-9491-y;
RA Moran Y., Praher D., Schlesinger A., Ayalon A., Tal Y., Technau U.;
RT "Analysis of soluble protein contents from the nematocysts of a model sea
RT anemone sheds light on venom evolution.";
RL Mar. Biotechnol. 15:329-339(2013).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND RECOMBINANT
RP EXPRESSION.
RX PubMed=29424690; DOI=10.7554/elife.35014;
RA Columbus-Shenkar Y.Y., Sachkova M.Y., Macrander J., Fridrich A.,
RA Modepalli V., Reitzel A.M., Sunagar K., Moran Y.;
RT "Dynamics of venom composition across a complex life cycle.";
RL Elife 7:0-0(2018).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=31134275; DOI=10.1093/molbev/msz132;
RA Sachkova M.Y., Singer S.A., Macrander J., Reitzel A.M., Peigneur S.,
RA Tytgat J., Moran Y.;
RT "The birth and death of toxins with distinct functions: a case study in the
RT sea anemone Nematostella.";
RL Mol. Biol. Evol. 36:2001-2012(2019).
CC -!- FUNCTION: Neurotoxin (PubMed:29424690). In vivo, induces pronounced
CC contraction and tail twitching on zebrafish larvae, as well as death 5
CC hours later (PubMed:29424690). {ECO:0000269|PubMed:29424690}.
CC -!- SUBCELLULAR LOCATION: Nematocyst {ECO:0000269|PubMed:23151943}.
CC Secreted {ECO:0000269|PubMed:23151943}.
CC -!- TISSUE SPECIFICITY: Nematocytes (PubMed:23151943, PubMed:29424690). In
CC late planulae, transcripts are found throughout the ectoderm in
CC nematocytes, with high concentration of expressing cells in the oral
CC pole (PubMed:29424690). In primary polyps, is expressed in nematocytes
CC in the body wall and physa ectoderm and in the upper and lower pharynx
CC (PubMed:29424690). {ECO:0000269|PubMed:23151943,
CC ECO:0000269|PubMed:29424690}.
CC -!- DEVELOPMENTAL STAGE: Higly expressed in late planulae, primary polyps,
CC and both adult males and females (at protein level) (PubMed:31134275).
CC Consistant with protein expression, transcripts are moderately
CC expressed in gastrulae and highly expressed in early and late planulae,
CC metamorphosing planulae, primary and juvenile polyps, and both adult
CC males and females (PubMed:29424690, PubMed:31134275). Low expression in
CC unfertilized eggs and blastulae (PubMed:31134275).
CC {ECO:0000269|PubMed:29424690, ECO:0000269|PubMed:31134275}.
CC -!- SIMILARITY: Belongs to the NEP3 family. {ECO:0000305}.
CC -!- CAUTION: The exact range of the peptide is deduced from experimental
CC results. It as been shown that the mature peptide weight is 10 kDa and
CC is only composed of the first ShKT domain.
CC {ECO:0000269|PubMed:29424690}.
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DR EMBL; DS469513; EDO48496.1; -; Genomic_DNA.
DR RefSeq; XP_001640559.1; XM_001640509.1.
DR STRING; 45351.EDO48496; -.
DR EnsemblMetazoa; EDO48496; EDO48496; NEMVEDRAFT_v1g238654.
DR eggNOG; KOG1217; Eukaryota.
DR HOGENOM; CLU_781441_0_0_1; -.
DR OrthoDB; 1917747at2759; -.
DR Proteomes; UP000001593; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR003582; ShKT_dom.
DR SMART; SM00254; ShKT; 2.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Disulfide bond; Nematocyst; Neurotoxin;
KW Reference proteome; Secreted; Signal; Toxin.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..91
FT /note="Nematocyst expressed protein 3"
FT /evidence="ECO:0000305|PubMed:29424690"
FT /id="PRO_5002711323"
FT PROPEP 92..355
FT /evidence="ECO:0000305|PubMed:29424690"
FT /id="PRO_0000453820"
FT DOMAIN 50..89
FT /note="ShKT 1"
FT /evidence="ECO:0000305|PubMed:29424690"
FT DOMAIN 107..134
FT /note="ShKT 2"
FT /evidence="ECO:0000305|PubMed:29424690"
FT DOMAIN 140..182
FT /note="ShKT 3"
FT /evidence="ECO:0000305|PubMed:29424690"
FT REGION 202..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..218
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..355
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 50..89
FT /evidence="ECO:0000305"
FT DISULFID 57..82
FT /evidence="ECO:0000305"
FT DISULFID 71..86
FT /evidence="ECO:0000305"
FT DISULFID 116..131
FT /evidence="ECO:0000305"
FT DISULFID 149..175
FT /evidence="ECO:0000305"
FT DISULFID 158..179
FT /evidence="ECO:0000305"
SQ SEQUENCE 355 AA; 38542 MW; 935C5B2E5052A858 CRC64;
MKLTYILLIA VVGVAIEAKS VKKSKAHHKK KRHETLADNY KLNRIKDSDC KDVADDCKAF
GKLEKDDENS CFNNPDKARN ECPVSCKLCV SKRSKKQSDY MGGYDLQPQQ CSQQSCYETP
QWSVQNCAVT CQLCQPGVSS GPVDQDVRCP FWGQYGYCSQ QQQQQQTDGY ISNNCPFSCN
TYGSAQPAQQ PYPYPIEALS PYQPNAMPTP PQGVTPAPLP PYFQQQGYGY PQQPQPTQPV
QPGQTQAPTA AQSTPAPVQT TPASGKTTTE AAEETTTEAA AEGAETTAAP AATQAPAAAG
QEGQQPAAGG QEPQEAQEQE GQQPGTEPAQ SDKSNKKKHK KDKAQKKSKS HKKQH
