NEP8_NEMVE
ID NEP8_NEMVE Reviewed; 313 AA.
AC A7SXG9;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 42.
DE RecName: Full=Nematocyst expressed protein 8 {ECO:0000303|PubMed:23151943};
DE Short=NEP-8 {ECO:0000303|PubMed:23151943};
DE Short=NEP8 {ECO:0000305};
DE Flags: Precursor;
GN ORFNames=v1g219037 {ECO:0000312|EMBL:EDO31595.1};
OS Nematostella vectensis (Starlet sea anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Edwardsiidae; Nematostella.
OX NCBI_TaxID=45351;
RN [1] {ECO:0000312|EMBL:EDO31595.1, ECO:0000312|Proteomes:UP000001593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CH2 X CH6 {ECO:0000312|Proteomes:UP000001593};
RX PubMed=17615350; DOI=10.1126/science.1139158;
RA Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA Technau U., Martindale M.Q., Rokhsar D.S.;
RT "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT genomic organization.";
RL Science 317:86-94(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23151943; DOI=10.1007/s10126-012-9491-y;
RA Moran Y., Praher D., Schlesinger A., Ayalon A., Tal Y., Technau U.;
RT "Analysis of soluble protein contents from the nematocysts of a model sea
RT anemone sheds light on venom evolution.";
RL Mar. Biotechnol. 15:329-339(2013).
RN [3]
RP PROBABLE FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=29424690; DOI=10.7554/elife.35014;
RA Columbus-Shenkar Y.Y., Sachkova M.Y., Macrander J., Fridrich A.,
RA Modepalli V., Reitzel A.M., Sunagar K., Moran Y.;
RT "Dynamics of venom composition across a complex life cycle.";
RL Elife 7:0-0(2018).
CC -!- FUNCTION: Probable toxin probably only used for predation.
CC {ECO:0000305|PubMed:29424690}.
CC -!- SUBCELLULAR LOCATION: Nematocyst {ECO:0000269|PubMed:23151943}.
CC Secreted {ECO:0000269|PubMed:23151943}.
CC -!- TISSUE SPECIFICITY: Nematocytes (PubMed:23151943, PubMed:29424690). In
CC late planulae, is only expressed in a handful of nematocytes in the
CC lower pharynx (PubMed:29424690). Is absent from the tentacles and outer
CC body wall (PubMed:29424690). {ECO:0000269|PubMed:23151943,
CC ECO:0000269|PubMed:29424690}.
CC -!- DEVELOPMENTAL STAGE: Transcripts are weakly expressed in gastrulae,
CC early and late planulae, metamorphosing planulae, and primary polyps.
CC {ECO:0000269|PubMed:29424690}.
CC -!- SIMILARITY: Belongs to the NEP3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDO31595.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DS469888; EDO31595.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001623695.1; XM_001623645.1.
DR SMR; A7SXG9; -.
DR EnsemblMetazoa; EDO31595; EDO31595; NEMVEDRAFT_v1g219037.
DR HOGENOM; CLU_683908_0_0_1; -.
DR OrthoDB; 1597243at2759; -.
DR Proteomes; UP000001593; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR003582; ShKT_dom.
DR Pfam; PF01549; ShK; 3.
DR SMART; SM00254; ShKT; 3.
DR PROSITE; PS51670; SHKT; 2.
PE 1: Evidence at protein level;
KW Disulfide bond; Nematocyst; Reference proteome; Repeat; Secreted; Signal;
KW Toxin.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..313
FT /note="Nematocyst expressed protein 8"
FT /evidence="ECO:0000305"
FT /id="PRO_0000453821"
FT DOMAIN 65..99
FT /note="ShKT 1"
FT /evidence="ECO:0000305|PubMed:29424690"
FT DOMAIN 109..145
FT /note="ShKT 2"
FT /evidence="ECO:0000305|PubMed:29424690"
FT DOMAIN 151..186
FT /note="ShKT 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT REGION 24..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..240
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..274
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..297
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 65..99
FT /evidence="ECO:0000305"
FT DISULFID 72..92
FT /evidence="ECO:0000305"
FT DISULFID 81..96
FT /evidence="ECO:0000305"
FT DISULFID 109..145
FT /evidence="ECO:0000305"
FT DISULFID 127..142
FT /evidence="ECO:0000305"
FT DISULFID 160..179
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT DISULFID 169..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ SEQUENCE 313 AA; 33926 MW; 58FB245FA3E9713D CRC64;
MLRRPLLLVL FTVFSTLYAK DLRGVSPPTN ESEAEVSPGD DEGPPEPGNE PDVNWRTLTI
DPEDCKDKGK DCESLADEGN CLKKLNYAVG NCPWTCRFCK KENGDSKKCK DLAGERHCNG
WKVKGDCVRL PDYMMQNCKL SCELCGPETR FKYTDEDVRC PEWAQAGYCS TNTDINLKCP
HSCRKYKQRA PASTPYPYPV EALHPYQYRV VLPSVTILQT TTAAPSTQPA ETTKAPPNTA
APTAAPTPAP TPAPAPAPTP APVAPAPQTA PLAGSPPEST PEEQDDNSAD ESTEIEAGEG
GGELCDEKHS SQQ
