NEPA_PAENI
ID NEPA_PAENI Reviewed; 116 AA.
AC Q8GAI5;
DT 11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Nicotine metabolites export pump subunit NepA;
DE AltName: Full=SMR efflux pump subunit NepA;
GN Name=nepA; ORFNames=ORF116, ORF61;
OS Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans).
OG Plasmid pAO1.
OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Paenarthrobacter.
OX NCBI_TaxID=29320;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 49919 / DSM 420 / JCM 3874 / NBRC 15511; PLASMID=pAO1;
RX PubMed=12618462; DOI=10.1128/jb.185.6.1976-1986.2003;
RA Igloi G.L., Brandsch R.;
RT "Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter
RT nicotinovorans and identification of a pAO1-dependent nicotine uptake
RT system.";
RL J. Bacteriol. 185:1976-1986(2003).
RN [2]
RP FUNCTION, GENE NAME, SUBUNIT, INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=ATCC 49919 / DSM 420 / JCM 3874 / NBRC 15511; PLASMID=pAO1;
RX PubMed=17464069; DOI=10.1099/mic.0.2006/004234-0;
RA Ganas P., Mihasan M., Igloi G.L., Brandsch R.;
RT "A two-component small multidrug resistance pump functions as a metabolic
RT valve during nicotine catabolism by Arthrobacter nicotinovorans.";
RL Microbiology 153:1546-1555(2007).
CC -!- FUNCTION: Component of an efflux pump responsible for the transport of
CC nicotine breakdown products, in particular methylamine, out of the
CC cell. This pump apparently serves as a metabolic valve for nicotine
CC catabolites and may protect the bacteria from the potentially toxic
CC side effects of these compounds. {ECO:0000269|PubMed:17464069}.
CC -!- SUBUNIT: The efflux pump is composed of NepA and NepB.
CC {ECO:0000269|PubMed:17464069}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Is transcribed only in the presence of nicotine under the
CC control of the transcriptional activator PmfR. Forms part of an operon
CC with nepB, folD, mabO and purU. {ECO:0000269|PubMed:17464069}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC NepA/NepB subfamily. {ECO:0000305}.
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DR EMBL; AJ507836; CAD47919.1; -; Genomic_DNA.
DR RefSeq; WP_016359430.1; NC_021229.1.
DR RefSeq; YP_007988745.1; NC_021229.1.
DR AlphaFoldDB; Q8GAI5; -.
DR SMR; Q8GAI5; -.
DR TCDB; 2.A.7.1.8; the drug/metabolite transporter (dmt) superfamily.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..116
FT /note="Nicotine metabolites export pump subunit NepA"
FT /id="PRO_0000429452"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 116 AA; 12440 MW; 8F99F0023221BC13 CRC64;
MQTRYGRRAL TIWPLLLLAI AAEVAATSLL PQTNGFRKLK PTVAVACLYT VAFALLAQIL
KFTDIGIAYA LWAGLGTASV AVIGVLFRNE RFSWKHAIGL ALVVTGVVTL NLQAGQ
