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NEPI_ECO57
ID   NEPI_ECO57              Reviewed;         396 AA.
AC   Q8XC49; Q7A9M0;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Purine ribonucleoside efflux pump NepI {ECO:0000255|HAMAP-Rule:MF_01189};
GN   Name=nepI {ECO:0000255|HAMAP-Rule:MF_01189};
GN   OrderedLocusNames=Z5149, ECs4596;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Involved in the efflux of purine ribonucleosides, such as
CC       inosine and guanosine. {ECO:0000255|HAMAP-Rule:MF_01189}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(out) + inosine(in) = H(+)(in) + inosine(out);
CC         Xref=Rhea:RHEA:29211, ChEBI:CHEBI:15378, ChEBI:CHEBI:17596;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29212;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(in) + H(+)(out) = guanosine(out) + H(+)(in);
CC         Xref=Rhea:RHEA:29583, ChEBI:CHEBI:15378, ChEBI:CHEBI:16750;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29584;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01189}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01189}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family.
CC       NepI (TC 2.A.1.2.26) subfamily. {ECO:0000255|HAMAP-Rule:MF_01189}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG58858.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB38019.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG58858.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB38019.1; ALT_INIT; Genomic_DNA.
DR   PIR; D91203; D91203.
DR   PIR; F86049; F86049.
DR   RefSeq; NP_312623.2; NC_002695.1.
DR   RefSeq; WP_001288545.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8XC49; -.
DR   SMR; Q8XC49; -.
DR   STRING; 155864.EDL933_4983; -.
DR   EnsemblBacteria; AAG58858; AAG58858; Z5149.
DR   EnsemblBacteria; BAB38019; BAB38019; ECs_4596.
DR   GeneID; 915429; -.
DR   KEGG; ece:Z5149; -.
DR   KEGG; ecs:ECs_4596; -.
DR   PATRIC; fig|386585.9.peg.4804; -.
DR   eggNOG; COG2814; Bacteria.
DR   HOGENOM; CLU_001265_61_1_6; -.
DR   OMA; LLMTGHF; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   HAMAP; MF_01189; MFS_NepI; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR023680; MFS_NepI.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..396
FT                   /note="Purine ribonucleoside efflux pump NepI"
FT                   /id="PRO_0000294109"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        43..54
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        76..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        107
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        129..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        169..175
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        197..215
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        237..255
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        277..281
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        303..305
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        327..343
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        365..366
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
FT   TOPO_DOM        388..396
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01189"
SQ   SEQUENCE   396 AA;  41851 MW;  17698C597FCE73D5 CRC64;
     MSEFIAENRG ADAITRPNWS AVFSVAFCVA CLIIVEFLPV SLLTPMAQDL GISEGVAGQS
     VTVTAFVAMF ASLFITQTIQ ATDRRYVVIL FAVLLTLSCL LVSFANSFSL LLIGRACLGL
     ALGGFWAMSA SLTMRLVPPR TVPKALSVIF GAVSIALVIA APLGCFLGEL IGWRNVFNAA
     AAMGVLCIFW IIKSLPSLPG EPSHQKQNTF RLLQRPGVMA GMIAIFMSFA GQFAFFTYIR
     PVYMTLAGFG VDGLTLVLLS FGIASFVGTS LSSFILKRSV KLALAGAPFV LALSALVLTL
     WGSDKIVATG VAIIWGLTFA LIPVGWSTWI TRSLADQAEK AGSIQVAVIQ LANTCGAAIG
     GYALDNIGLT SPLMLSGTLM LLTALLVTAK VKMKKS
 
 
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