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NEPI_ECOLI
ID   NEPI_ECOLI              Reviewed;         396 AA.
AC   P0ADL1; P31438; P76725; Q2M7X5; Q5I3I1;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Purine ribonucleoside efflux pump NepI {ECO:0000255|HAMAP-Rule:MF_01189, ECO:0000305};
DE   AltName: Full=Nucleoside efflux permease-inosine {ECO:0000303|PubMed:16040204};
GN   Name=nepI {ECO:0000255|HAMAP-Rule:MF_01189, ECO:0000303|PubMed:16040204};
GN   Synonyms=yicM; OrderedLocusNames=b3662, JW5938;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA   Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT   "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT   organizational symmetry around the origin of replication.";
RL   Genomics 16:551-561(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-396.
RC   STRAIN=BEN2908 / O2:K1:H5 / APEC;
RX   PubMed=16428402; DOI=10.1128/jb.188.3.977-987.2006;
RA   Chouikha I., Germon P., Bree A., Gilot P., Moulin-Schouleur M.,
RA   Schouler C.;
RT   "A selC-associated genomic island of the extraintestinal avian pathogenic
RT   Escherichia coli strain BEN2908 is involved in carbohydrate uptake and
RT   virulence.";
RL   J. Bacteriol. 188:977-987(2006).
RN   [5]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
RN   [6]
RP   FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, INDUCTION, DISRUPTION
RP   PHENOTYPE, AND CHARACTERIZATION OF TRANSLATIONAL START SITE.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=16040204; DOI=10.1016/j.femsle.2005.06.051;
RA   Gronskiy S.V., Zakataeva N.P., Vitushkina M.V., Ptitsyn L.R., Altman I.B.,
RA   Novikova A.E., Livshits V.A.;
RT   "The yicM (nepI) gene of Escherichia coli encodes a major facilitator
RT   superfamily protein involved in efflux of purine ribonucleosides.";
RL   FEMS Microbiol. Lett. 250:39-47(2005).
CC   -!- FUNCTION: Involved in the efflux of purine ribonucleosides, such as
CC       inosine and guanosine (PubMed:16040204). Adenosine may also be a
CC       substrate (PubMed:16040204). Confers resistance to the hypoxanthine
CC       analog 6-mercaptopurine, however the level of resistance is rather low
CC       (PubMed:16040204). {ECO:0000269|PubMed:16040204}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(out) + inosine(in) = H(+)(in) + inosine(out);
CC         Xref=Rhea:RHEA:29211, ChEBI:CHEBI:15378, ChEBI:CHEBI:17596;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189,
CC         ECO:0000269|PubMed:16040204};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29212;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189,
CC         ECO:0000269|PubMed:16040204};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(in) + H(+)(out) = guanosine(out) + H(+)(in);
CC         Xref=Rhea:RHEA:29583, ChEBI:CHEBI:15378, ChEBI:CHEBI:16750;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189,
CC         ECO:0000269|PubMed:16040204};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29584;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01189,
CC         ECO:0000269|PubMed:16040204};
CC   -!- ACTIVITY REGULATION: Reducing the electrochemical proton gradient
CC       across the plasma membrane by the addition of the protonophore carbonyl
CC       cyanide m-chlorophenylhydrazone (CCCP) leads to a drastic reduction in
CC       the rate of inosine excretion. {ECO:0000269|PubMed:16040204}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01189, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: In stationary phase. {ECO:0000269|PubMed:16040204}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation of the gene leads to increased
CC       susceptibility to purine ribonucleosides.
CC       {ECO:0000269|PubMed:16040204}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family.
CC       NepI (TC 2.A.1.2.26) subfamily. {ECO:0000255|HAMAP-Rule:MF_01189,
CC       ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA62014.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAW51767.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAE77631.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; L10328; AAA62014.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC76685.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77631.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AY857617; AAW51767.1; ALT_FRAME; Genomic_DNA.
DR   PIR; G65167; G65167.
DR   RefSeq; NP_418118.2; NC_000913.3.
DR   RefSeq; WP_001288549.1; NZ_SSZK01000043.1.
DR   AlphaFoldDB; P0ADL1; -.
DR   SMR; P0ADL1; -.
DR   BioGRID; 4261472; 10.
DR   DIP; DIP-48180N; -.
DR   STRING; 511145.b3662; -.
DR   TCDB; 2.A.1.2.26; the major facilitator superfamily (mfs).
DR   PaxDb; P0ADL1; -.
DR   PRIDE; P0ADL1; -.
DR   EnsemblBacteria; AAC76685; AAC76685; b3662.
DR   EnsemblBacteria; BAE77631; BAE77631; BAE77631.
DR   GeneID; 66672441; -.
DR   GeneID; 948213; -.
DR   KEGG; ecj:JW5938; -.
DR   KEGG; eco:b3662; -.
DR   PATRIC; fig|511145.12.peg.3784; -.
DR   EchoBASE; EB1640; -.
DR   eggNOG; COG2814; Bacteria.
DR   HOGENOM; CLU_001265_61_1_6; -.
DR   InParanoid; P0ADL1; -.
DR   PhylomeDB; P0ADL1; -.
DR   BioCyc; EcoCyc:YICM-MON; -.
DR   BioCyc; MetaCyc:YICM-MON; -.
DR   PRO; PR:P0ADL1; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IMP:EcoCyc.
DR   GO; GO:0015860; P:purine nucleoside transmembrane transport; IMP:EcoCyc.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   HAMAP; MF_01189; MFS_NepI; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR023680; MFS_NepI.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..396
FT                   /note="Purine ribonucleoside efflux pump NepI"
FT                   /id="PRO_0000169616"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        43..54
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        76..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        107
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        129..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..175
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        197..215
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        237..255
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        277..281
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        303..305
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        327..343
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        365..366
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        367..387
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        388..396
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000269|PubMed:15919996"
FT   VARIANT         158
FT                   /note="V -> M (in strain: BEN2908 / O2:K1:H5 / APEC)"
FT   VARIANT         182
FT                   /note="V -> A (in strain: BEN2908 / O2:K1:H5 / APEC)"
FT   VARIANT         267
FT                   /note="I -> V (in strain: BEN2908 / O2:K1:H5 / APEC)"
FT   VARIANT         289..290
FT                   /note="LI -> FV (in strain: BEN2908 / O2:K1:H5 / APEC)"
FT   VARIANT         293
FT                   /note="V -> L (in strain: BEN2908 / O2:K1:H5 / APEC)"
FT   VARIANT         322
FT                   /note="V -> I (in strain: BEN2908 / O2:K1:H5 / APEC)"
SQ   SEQUENCE   396 AA;  41842 MW;  DB240A4A8C1B9D1B CRC64;
     MSEFIAENRG ADAITRPNWS AVFSVAFCVA CLIIVEFLPV SLLTPMAQDL GISEGVAGQS
     VTVTAFVAMF ASLFITQTIQ ATDRRYVVIL FAVLLTLSCL LVSFANSFSL LLIGRACLGL
     ALGGFWAMSA SLTMRLVPPR TVPKALSVIF GAVSIALVIA APLGSFLGEL IGWRNVFNAA
     AVMGVLCIFW IIKSLPSLPG EPSHQKQNTF RLLQRPGVMA GMIAIFMSFA GQFAFFTYIR
     PVYMNLAGFG VDGLTLVLLS FGIASFIGTS LSSFILKRSV KLALAGAPLI LAVSALVLTL
     WGSDKIVATG VAIIWGLTFA LVPVGWSTWI TRSLADQAEK AGSIQVAVIQ LANTCGAAIG
     GYALDNIGLT SPLMLSGTLM LLTALLVTAK VKMKKS
 
 
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