ID   NEPRO_MOUSE             Reviewed;         564 AA.
AC   Q8R2U2;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Nucleolus and neural progenitor protein {ECO:0000305};
DE   AltName: Full=Neural progenitor protein {ECO:0000303|PubMed:19906856};
GN   Name=Nepro {ECO:0000303|PubMed:19906856, ECO:0000312|MGI:MGI:2384836};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND REGION.
RX   PubMed=19906856; DOI=10.1242/dev.039180;
RA   Muroyama Y., Saito T.;
RT   "Identification of Nepro, a gene required for the maintenance of neocortex
RT   neural progenitor cells downstream of Notch.";
RL   Development 136:3889-3893(2009).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=26178919; DOI=10.1111/dgd.12232;
RA   Hashimoto M., Sato T., Muroyama Y., Fujimura L., Hatano M., Saito T.;
RT   "Nepro is localized in the nucleolus and essential for preimplantation
RT   development in mice.";
RL   Dev. Growth Differ. 57:529-538(2015).
CC   -!- FUNCTION: May play a role in cortex development as part of the Notch
CC       signaling pathway. Downstream of Notch may repress the expression of
CC       proneural genes and inhibit neuronal differentiation thereby
CC       maintaining neural progenitors (PubMed:19906856). May also play a role
CC       in preimplentation embryo development (PubMed:26178919).
CC       {ECO:0000269|PubMed:19906856, ECO:0000269|PubMed:26178919}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19906856}. Nucleus,
CC       nucleolus {ECO:0000269|PubMed:26178919}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in all blastomeres at the 8-cell stage
CC       (PubMed:26178919). Detected in the ventricular zone (VZ) of the
CC       forebrain at 9.5 dpc. Clearly detected until 12.5 dpc, the expression
CC       decreases and disappears by 15.5 dpc (PubMed:19906856).
CC       {ECO:0000269|PubMed:19906856, ECO:0000269|PubMed:26178919}.
CC   -!- DISRUPTION PHENOTYPE: Knockout embryos die before preimplantation.
CC       {ECO:0000269|PubMed:26178919}.
CC   -!- SIMILARITY: Belongs to the nepro family. {ECO:0000305}.
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DR   EMBL; AK144612; BAE25969.1; -; mRNA.
DR   EMBL; AC122217; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC124180; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH466521; EDK98047.1; -; Genomic_DNA.
DR   EMBL; BC027231; AAH27231.1; -; mRNA.
DR   CCDS; CCDS28187.1; -.
DR   RefSeq; NP_666084.1; NM_145972.4.
DR   AlphaFoldDB; Q8R2U2; -.
DR   STRING; 10090.ENSMUSP00000038779; -.
DR   iPTMnet; Q8R2U2; -.
DR   PhosphoSitePlus; Q8R2U2; -.
DR   EPD; Q8R2U2; -.
DR   MaxQB; Q8R2U2; -.
DR   PaxDb; Q8R2U2; -.
DR   PeptideAtlas; Q8R2U2; -.
DR   PRIDE; Q8R2U2; -.
DR   ProteomicsDB; 287479; -.
DR   Antibodypedia; 32543; 91 antibodies from 18 providers.
DR   DNASU; 212547; -.
DR   Ensembl; ENSMUST00000048788; ENSMUSP00000038779; ENSMUSG00000036208.
DR   GeneID; 212547; -.
DR   KEGG; mmu:212547; -.
DR   UCSC; uc007zho.2; mouse.
DR   CTD; 25871; -.
DR   MGI; MGI:2384836; Nepro.
DR   VEuPathDB; HostDB:ENSMUSG00000036208; -.
DR   eggNOG; ENOG502QTP3; Eukaryota.
DR   GeneTree; ENSGT00390000007644; -.
DR   HOGENOM; CLU_035014_0_0_1; -.
DR   InParanoid; Q8R2U2; -.
DR   OMA; INKHNTS; -.
DR   OrthoDB; 480212at2759; -.
DR   PhylomeDB; Q8R2U2; -.
DR   TreeFam; TF335999; -.
DR   BioGRID-ORCS; 212547; 9 hits in 43 CRISPR screens.
DR   PRO; PR:Q8R2U2; -.
DR   Proteomes; UP000000589; Chromosome 16.
DR   RNAct; Q8R2U2; protein.
DR   Bgee; ENSMUSG00000036208; Expressed in cerebral cortex marginal layer and 221 other tissues.
DR   ExpressionAtlas; Q8R2U2; baseline and differential.
DR   GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0045665; P:negative regulation of neuron differentiation; IDA:MGI.
DR   GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:MGI.
DR   InterPro; IPR027951; DUF4477.
DR   Pfam; PF14780; DUF4477; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Nucleus; Reference proteome.
FT   CHAIN           1..564
FT                   /note="Nucleolus and neural progenitor protein"
FT                   /id="PRO_0000434895"
FT   REGION          431..495
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          442..460
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000303|PubMed:19906856"
FT   COMPBIAS        457..479
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   564 AA;  63394 MW;  4D945DBC5F0FB622 CRC64;
     MAAAVRPGAE PWNRVRIPQA GNCSTLTVRD PSATLDICTA AVTKGCHLVT QSLKSQTLDA
     EVDVLCSVLY SNHNRLGHHK PHLALRQVEQ CLKRLKHMNL EGSIEDLSQL LSANATQPGA
     TENRVVPSQP VVEVVLMKVL GGCKLLLRLL DCCCKAFLLT VKHLGLKEFI ILNLVMVGLV
     SRLWVLHKGL LRRLISLYEP LLSLRQEISS IHPMPYFKDF AFPSDITDFL GPSYLEVFKV
     KTPAASATKG VTKLLNKLFL MREQLPKMNE DTLDRLSKPS EQMTSNPQST VDLGQPVKAC
     KRTRKEKPLG FDLRAFCTRL GNKATQETNR DFKYSQSKLK TTKLPSQQLR THWANDTVQR
     IRKTKTFAQL SEEIEMAIVW SRSKKLKTQA TFLGNKLLKS NRFRHVESQG YSLTKKLQCM
     KTSLCNCLLR GSRTSTSEHP PRQRRSKYKV LSRQRKPQRK LQSTLLKETQ QVPEGTLKNT
     RDSSAKRRCS GTVQRSDVCP NGKQVLRKLA KPDLKTKVVV HGNLTGGSRN ESGFQAKTQM
     HTHNAPDTAK EADDIDDIFA LMGV