ID   NEP_I02A7               Reviewed;         111 AA.
AC   Q6DP36;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   29-SEP-2021, entry version 56.
DE   RecName: Full=Nuclear export protein;
DE            Short=NEP;
DE   AltName: Full=Non-structural protein 2;
DE            Short=NS2;
DE   Flags: Fragment;
GN   Name=NS;
OS   Influenza A virus (strain A/Teal/China/2978.1/2002 H5N1 genotype W).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=284215;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15241415; DOI=10.1038/nature02746;
RA   Li K.S., Guan Y., Wang J., Smith G.J.D., Xu K.M., Duan L., Rahardjo A.P.,
RA   Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T.S.,
RA   Chaisingh A., Auewarakul P., Long H.T., Hanh N.T.H., Webby R.J.,
RA   Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G.,
RA   Peiris J.S.M.;
RT   "Genesis of a highly pathogenic and potentially pandemic H5N1 influenza
RT   virus in eastern Asia.";
RL   Nature 430:209-213(2004).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs to which
CC       the M1 protein is bound. Because the M1 protein is not available in
CC       large quantities until the later stages of infection, such an indirect
CC       recognition mechanism probably ensures that genomic RNPs are not
CC       exported from the nucleus before sufficient quantities of viral mRNA
CC       and progeny genomic RNA have been synthesized. Furthermore, the RNPs
CC       enters the cytoplasm only when they have associated with the M1 protein
CC       that is necessary to guide them to the plasma membrane. May down-
CC       regulate viral RNA synthesis when overproduced (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Binds M1 protein. May interact with human nucleoporin RAB/HRB
CC       and exportin XPO1/CRM1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=Q6DP36-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=Q6DP37-1; Sequence=External;
CC   -!- MISCELLANEOUS: Average number present in a viral particle is estimated
CC       to be 130-200 molecules.
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family. {ECO:0000305}.
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DR   EMBL; AY651581; AAT73450.1; -; Genomic_RNA.
DR   SMR; Q6DP36; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:InterPro.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF00601; Flu_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           <1..111
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000311738"
FT   MOTIF           2..11
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           75..84
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   111 AA;  13301 MW;  425E5701E4667311 CRC64;
     DILMRMSKMQ LGSSSEDLNG MITRFESLKL YRDSLGEAVM RMGDLHSLQT RNGKWREQLS
     QKFEEIRWLI EEVRHRLKIT ENSFEQITFM QALQLLLEVE QEMRTFSFQL I