ID   NEP_I05A1               Reviewed;         117 AA.
AC   P0C5U4;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=Nuclear export protein;
DE            Short=NEP;
DE   AltName: Full=Non-structural protein 2;
DE            Short=NS2;
DE   Flags: Fragment;
GN   Name=NS;
OS   Influenza A virus (strain A/Goose/Guangxi/345/2005 H5N1 genotype G).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=365089;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17075062; DOI=10.1073/pnas.0608157103;
RA   Smith G.J., Fan X.H., Wang J., Li K.S., Qin K., Zhang J.X.,
RA   Vijaykrishna D., Cheung C.L., Huang K., Rayner J.M., Peiris J.S., Chen H.,
RA   Webster R.G., Guan Y.;
RT   "Emergence and predominance of an H5N1 influenza variant in China.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:16936-16941(2006).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs to which
CC       the M1 protein is bound. Because the M1 protein is not available in
CC       large quantities until the later stages of infection, such an indirect
CC       recognition mechanism probably ensures that genomic RNPs are not
CC       exported from the nucleus before sufficient quantities of viral mRNA
CC       and progeny genomic RNA have been synthesized. Furthermore, the RNPs
CC       enters the cytoplasm only when they have associated with the M1 protein
CC       that is necessary to guide them to the plasma membrane. May down-
CC       regulate viral RNA synthesis when overproduced (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Binds M1 protein. May interact with human nucleoporin RAB/HRB
CC       and exportin XPO1/CRM1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=P0C5U4-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=Q2F4N6-1; Sequence=External;
CC   -!- MISCELLANEOUS: Average number present in a viral particle is estimated
CC       to be 130-200 molecules.
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family. {ECO:0000305}.
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DR   EMBL; DQ321159; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   SMR; P0C5U4; -.
DR   PRIDE; P0C5U4; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:InterPro.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF00601; Flu_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           <1..117
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000311741"
FT   MOTIF           8..17
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           81..90
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   117 AA;  14018 MW;  A25C956BF9F07C45 CRC64;
     TVSSFQDILM RMSKMQLGYS SEDLNGMITR FESLKLYRDS LGEAVIRMGD LHSLQTRNGK
     WREQLSHKFE EIRWLIEEVR HRLKITENSF EQITFMQALQ LLLEVEQEMR TFSFQLI