ID   NEP_I47A0               Reviewed;         121 AA.
AC   P03506; Q77AL4;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Nuclear export protein {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NEP {ECO:0000255|HAMAP-Rule:MF_04067};
DE   AltName: Full=Non-structural protein 2 {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NS2 {ECO:0000255|HAMAP-Rule:MF_04067};
GN   Name=NS {ECO:0000255|HAMAP-Rule:MF_04067};
OS   Influenza A virus (strain A/Fort Monmouth/1/1947 H1N1).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=380282;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=A/Fort Monmouth/1/1947-MA;
RX   PubMed=6834468; DOI=10.1128/jvi.45.2.547-554.1983;
RA   Krystal M., Buonagurio D.A., Young J.F., Palese P.;
RT   "Sequential mutations in the NS genes of influenza virus field strains.";
RL   J. Virol. 45:547-554(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8254767; DOI=10.1128/jvi.68.1.530-534.1994;
RA   Smeenk C.A., Brown E.G.;
RT   "The influenza virus variant A/FM/1/47-MA possesses single amino acid
RT   replacements in the hemagglutinin, controlling virulence, and in the matrix
RT   protein, controlling virulence as well as growth.";
RL   J. Virol. 68:530-534(1994).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs bound to
CC       the protein M1. Since protein M1 is not available in large quantities
CC       before late stages of infection, such an indirect recognition mechanism
CC       probably ensures that genomic RNPs are not exported from the host
CC       nucleus until sufficient quantities of viral mRNA and progeny genomic
CC       RNA have been synthesized. Furthermore, the RNPs enter the host
CC       cytoplasm only when associated with the M1 protein that is necessary to
CC       guide them to the plasma membrane. May down-regulate viral RNA
CC       synthesis when overproduced. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBUNIT: Interacts with protein M1. May interact with host nucleoporin
CC       RAB/HRB and exportin XPO1/CRM1. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04067}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=P03506-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=P03499-1; Sequence=External;
CC   -!- MISCELLANEOUS: Average number present in a viral particle is estimated
CC       to be 130-200 molecules.
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family.
CC       {ECO:0000255|HAMAP-Rule:MF_04067}.
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DR   EMBL; K00577; AAA43521.1; -; Genomic_RNA.
DR   EMBL; U02087; AAA67340.1; -; Genomic_RNA.
DR   EMBL; AJ238022; CAB50888.1; -; Genomic_RNA.
DR   SMR; P03506; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04067; INFV_NEP; 1.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF00601; Flu_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           1..121
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000078985"
FT   MOTIF           12..21
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   MOTIF           85..94
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   VARIANT         31
FT                   /note="V -> M (in strain: A/Fort Monmouth/1/1947-MA)"
FT   VARIANT         34
FT                   /note="L -> Q (in strain: A/Fort Monmouth/1/1947-MA)"
FT   VARIANT         46
FT                   /note="G -> E (in strain: A/Fort Monmouth/1/1947-MA)"
FT   VARIANT         50..52
FT                   /note="VRM -> MRL (in strain: A/Fort Monmouth/1/1947-MA)"
FT   VARIANT         58
FT                   /note="L -> F (in strain: A/Fort Monmouth/1/1947-MA)"
FT   VARIANT         107
FT                   /note="F -> L (in strain: A/Fort Monmouth/1/1947-MA)"
SQ   SEQUENCE   121 AA;  14300 MW;  ED67C64D21C9BE9F CRC64;
     MDPNTVSSFQ DILMRMSKMQ LGSSSEDLNG VITLFESLKL YRDSLGEAVV RMGDLHSLQN
     RNGKWREQLG QKFEEIRWLI EEVRHRLKIT ENSFEQITFM QALQLLFEVE QEIRTFSFQL
     I