ID   NEP_I68A4               Reviewed;         121 AA.
AC   Q910E4; Q91M98;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Nuclear export protein {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NEP {ECO:0000255|HAMAP-Rule:MF_04067};
DE   AltName: Full=Non-structural protein 2 {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NS2 {ECO:0000255|HAMAP-Rule:MF_04067};
GN   Name=NS {ECO:0000255|HAMAP-Rule:MF_04067};
OS   Influenza A virus (strain A/Hong Kong/1/1968 H3N2).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=506350;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9721; Cetacea (whales).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9709; Phocidae (true seals).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=A/Hong Kong/1/1968, and Isolate MA20b;
RX   PubMed=11371620; DOI=10.1073/pnas.111165798;
RA   Brown E.G., Liu H., Kit L.C., Baird S., Nesrallah M.;
RT   "Pattern of mutation in the genome of influenza A virus on adaptation to
RT   increased virulence in the mouse lung: identification of functional
RT   themes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:6883-6888(2001).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs bound to
CC       the protein M1. Since protein M1 is not available in large quantities
CC       before late stages of infection, such an indirect recognition mechanism
CC       probably ensures that genomic RNPs are not exported from the host
CC       nucleus until sufficient quantities of viral mRNA and progeny genomic
CC       RNA have been synthesized. Furthermore, the RNPs enter the host
CC       cytoplasm only when associated with the M1 protein that is necessary to
CC       guide them to the plasma membrane. May down-regulate viral RNA
CC       synthesis when overproduced. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBUNIT: Interacts with protein M1. May interact with host nucleoporin
CC       RAB/HRB and exportin XPO1/CRM1. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04067}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=Q910E4-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=Q91MA0-1; Sequence=External;
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family.
CC       {ECO:0000255|HAMAP-Rule:MF_04067}.
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DR   EMBL; AF348198; AAK51751.1; -; Genomic_RNA.
DR   EMBL; AF348199; AAK51753.1; -; Genomic_RNA.
DR   EMBL; AF348201; AAK51757.1; -; Genomic_RNA.
DR   EMBL; AF348200; AAK51755.1; -; Genomic_RNA.
DR   EMBL; AF348202; AAK51759.1; -; Genomic_RNA.
DR   EMBL; AF348203; AAK51761.1; -; Genomic_RNA.
DR   EMBL; AF348204; AAK51763.1; -; Genomic_RNA.
DR   EMBL; AF348205; AAK51765.1; -; Genomic_RNA.
DR   EMBL; AF348206; AAK51767.1; -; Genomic_RNA.
DR   SMR; Q910E4; -.
DR   Proteomes; UP000142359; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04067; INFV_NEP; 1.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF00601; Flu_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           1..121
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000324202"
FT   MOTIF           12..21
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   MOTIF           85..94
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   VARIANT         88
FT                   /note="K -> R (in strain: Isolate MA20b)"
SQ   SEQUENCE   121 AA;  14339 MW;  E5C50200C3A14588 CRC64;
     MDSNTVSSFQ DILLRMSKMQ LGSSSEDLNG MITQFESLKL YRDSLGEAVM RMGDLHSLQN
     RNGKWREQLG QKFEEIRWLI EEVRHRLKTT ENSFEQITFM QALQLLFEVE QEIRTFSFQL
     I