ID   NEP_I73A4               Reviewed;         112 AA.
AC   O09686;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   29-SEP-2021, entry version 73.
DE   RecName: Full=Nuclear export protein;
DE            Short=NEP;
DE   AltName: Full=Non structural protein 2;
DE            Short=NS2;
DE   Flags: Fragment;
GN   Name=NS;
OS   Influenza A virus (strain A/Equine/London/1416/1973 H7N7).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=380340;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9796; Equus caballus (Horse).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9709; Phocidae (true seals).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8892928; DOI=10.1128/jvi.70.11.8041-8046.1996;
RA   Guan Y., Shortridge K.F., Krauss S., Li P.H., Kawaoka Y., Webster R.G.;
RT   "Emergence of avian H1N1 influenza viruses in pigs in China.";
RL   J. Virol. 70:8041-8046(1996).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs to which
CC       the M1 protein is bound. Because the M1 protein is not available in
CC       large quantities until the later stages of infection, such an indirect
CC       recognition mechanism probably ensures that genomic RNPs are not
CC       exported from the nucleus before sufficient quantities of viral mRNA
CC       and progeny genomic RNA have been synthesized. Furthermore, the RNPs
CC       enters the cytoplasm only when they have associated with the M1 protein
CC       that is necessary to guide them to the plasma membrane. May down-
CC       regulate viral RNA synthesis when overproduced (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Binds M1 protein. May interact with human nucleoporin RAB/HRB
CC       and exportin XPO1/CRM1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=O09686-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=O09687-1; Sequence=External;
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family. {ECO:0000305}.
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DR   EMBL; U49486; AAB51000.1; -; Genomic_RNA.
DR   SMR; O09686; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:InterPro.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF00601; Flu_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           1..112
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000324210"
FT   MOTIF           9..18
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           82..91
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         112
SQ   SEQUENCE   112 AA;  13308 MW;  590285174DD7812D CRC64;
     NTVSSFQDIL MRMSKMQLGS SSEDLNGMIT QLESLKLYRD SLGEAVMRMG DLHSLQSRNE
     KWREQLSQKF EEIRWLIEEV RHRLKNTENS FEQITFMQAL QLLLEVEQEI RT