ID   NEP_I97A1               Reviewed;         121 AA.
AC   O56263; Q778E8; Q9YX77;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Nuclear export protein {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NEP {ECO:0000255|HAMAP-Rule:MF_04067};
DE   AltName: Full=Non-structural protein 2 {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NS2 {ECO:0000255|HAMAP-Rule:MF_04067};
GN   Name=NS {ECO:0000255|HAMAP-Rule:MF_04067};
OS   Influenza A virus (strain A/Hong Kong/156/1997 H5N1 genotype Gs/Gd).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus.
OX   NCBI_TaxID=130763;
OH   NCBI_TaxID=8782; Aves.
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9691; Panthera pardus (Leopard) (Felis pardus).
OH   NCBI_TaxID=9694; Panthera tigris (Tiger).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9430591; DOI=10.1126/science.279.5349.393;
RA   Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M.,
RA   Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X.,
RA   Fukuda K., Cox N.;
RT   "Characterization of an avian influenza A (H5N1) virus isolated from a
RT   child with a fatal respiratory illness.";
RL   Science 279:393-396(1998).
RN   [2]
RP   SEQUENCE REVISION.
RA   Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M.,
RA   Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X.,
RA   Fukuda K., Cox N.;
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=9658115; DOI=10.1128/jvi.72.8.6678-6688.1998;
RA   Suarez D.L., Perdue M.L., Cox N., Rowe T., Bender C., Huang J.,
RA   Swayne D.E.;
RT   "Comparisons of highly virulent H5N1 influenza A viruses isolated from
RT   humans and chickens from Hong Kong.";
RL   J. Virol. 72:6678-6688(1998).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10920197; DOI=10.1073/pnas.160270697;
RA   Lin Y.P., Shaw M., Gregory V., Cameron K., Lim W., Klimov A., Subbarao K.,
RA   Guan Y., Krauss S., Shortridge K., Webster R., Cox N., Hay A.;
RT   "Avian-to-human transmission of H9N2 subtype influenza A viruses:
RT   relationship between H9N2 and H5N1 human isolates.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:9654-9658(2000).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs bound to
CC       the protein M1. Since protein M1 is not available in large quantities
CC       before late stages of infection, such an indirect recognition mechanism
CC       probably ensures that genomic RNPs are not exported from the host
CC       nucleus until sufficient quantities of viral mRNA and progeny genomic
CC       RNA have been synthesized. Furthermore, the RNPs enter the host
CC       cytoplasm only when associated with the M1 protein that is necessary to
CC       guide them to the plasma membrane. May down-regulate viral RNA
CC       synthesis when overproduced. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBUNIT: Interacts with protein M1. May interact with host nucleoporin
CC       RAB/HRB and exportin XPO1/CRM1. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04067}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=O56263-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=O56264-1; Sequence=External;
CC   -!- MISCELLANEOUS: Average number present in a viral particle is estimated
CC       to be 130-200 molecules.
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family.
CC       {ECO:0000255|HAMAP-Rule:MF_04067}.
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DR   EMBL; AF036360; AAC34269.2; -; Genomic_RNA.
DR   EMBL; AF046091; AAC32093.1; -; Genomic_RNA.
DR   EMBL; AJ404736; CAC04090.1; -; Genomic_RNA.
DR   SMR; O56263; -.
DR   Proteomes; UP000008587; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04067; INFV_NEP; 1.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF00601; Flu_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           1..121
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000078991"
FT   MOTIF           12..21
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   MOTIF           85..94
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
SQ   SEQUENCE   121 AA;  14390 MW;  A5381BD935178D31 CRC64;
     MDSNTVSSFQ DILKRMSKMQ LGSSSEDLNG MITQFESLKL YRDSLGEAVM RMGDLHSLQN
     RNGKWREQLS QKFEEIRWLI EEVRHRLRIT ENSFEQITFM QALQLLLEVE QEIRTFSFQL
     I