ID   NEP_INCAA               Reviewed;         182 AA.
AC   Q01640; A7BHX7;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   05-APR-2011, sequence version 3.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Nuclear export protein {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NEP {ECO:0000255|HAMAP-Rule:MF_04067};
DE   AltName: Full=Non-structural protein 2 {ECO:0000255|HAMAP-Rule:MF_04067};
DE            Short=NS2 {ECO:0000255|HAMAP-Rule:MF_04067};
GN   Name=NS {ECO:0000255|HAMAP-Rule:MF_04067};
OS   Influenza C virus (strain C/Ann Arbor/1/1950).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX   NCBI_TaxID=11553;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=17537859; DOI=10.1128/jvi.00075-07;
RA   Muraki Y., Murata T., Takashita E., Matsuzaki Y., Sugawara K., Hongo S.;
RT   "A mutation on influenza C virus M1 protein affects virion morphology by
RT   altering the membrane affinity of the protein.";
RL   J. Virol. 81:8766-8773(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 6-182.
RX   PubMed=2943076; DOI=10.1016/0042-6822(86)90003-6;
RA   Buonagurio D.A., Nakada S., Fitch W.M., Palese P.;
RT   "Epidemiology of influenza C virus in man: multiple evolutionary lineages
RT   and low rate of change.";
RL   Virology 153:12-21(1986).
RN   [3]
RP   IDENTIFICATION OF FRAMESHIFT.
RX   PubMed=10900030; DOI=10.1099/0022-1317-81-8-1933;
RA   Alamgir A.S.M., Matsuzaki Y., Hongo S., Tsuchiya E., Sugawara K.,
RA   Muraki Y., Nakamura K.;
RT   "Phylogenetic analysis of influenza C virus nonstructural (NS) protein
RT   genes and identification of the NS2 protein.";
RL   J. Gen. Virol. 81:1933-1940(2000).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs bound to
CC       the protein M1. Since protein M1 is not available in large quantities
CC       before late stages of infection, such an indirect recognition mechanism
CC       probably ensures that genomic RNPs are not exported from the host
CC       nucleus until sufficient quantities of viral mRNA and progeny genomic
CC       RNA have been synthesized. Furthermore, the RNPs enter the host
CC       cytoplasm only when associated with the M1 protein that is necessary to
CC       guide them to the plasma membrane. May down-regulate viral RNA
CC       synthesis when overproduced. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBUNIT: Interacts with protein M1. May interact with host nucleoporin
CC       RAB/HRB and exportin XPO1/CRM1. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04067}. Host
CC       nucleus {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=Q01640-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=Q01639-1; Sequence=External;
CC   -!- SIMILARITY: Belongs to the influenza viruses NEP family.
CC       {ECO:0000255|HAMAP-Rule:MF_04067}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA24045.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AB283001; BAF74589.1; -; Viral_cRNA.
DR   EMBL; D00029; BAA24045.1; ALT_FRAME; Genomic_RNA.
DR   RefSeq; YP_002302328.1; NC_006306.2. [Q01640-1]
DR   SMR; Q01640; -.
DR   DNASU; 7011833; -.
DR   GeneID; 7011833; -.
DR   KEGG; vg:7011833; -.
DR   Proteomes; UP000008286; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04067; INFV_NEP; 1.
DR   InterPro; IPR005188; Flu_C_NS2.
DR   InterPro; IPR000968; Flu_NS2.
DR   Pfam; PF03555; Flu_C_NS2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Host nucleus; Host-virus interaction;
KW   Reference proteome; Transport; Virion.
FT   CHAIN           1..182
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000079017"
FT   MOTIF           96..105
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   MOTIF           122..132
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT   VARIANT         169
FT                   /note="N -> T"
SQ   SEQUENCE   182 AA;  20768 MW;  2D44CC3CD1FB54E0 CRC64;
     MSDKTVKSTN LMAFVATKML ERQEDLDTCT EMQVEKMKTS TKARLKTESS FAPRTWEDAI
     KDEILRRSVD TSSLDKWPEL KQELENVSDA LKADSLWLPM KSLSLYSKVS NQEPSSIPIG
     EMKHQILTRL KLICSRLEKL DLNLSKAVLG IQNSEDLILI IYNRDVCKNT ILMIKSLCNS
     LI