NEP_INCCA
ID NEP_INCCA Reviewed; 182 AA.
AC P33493; Q68BV5;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 2.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Nuclear export protein {ECO:0000255|HAMAP-Rule:MF_04067};
DE Short=NEP {ECO:0000255|HAMAP-Rule:MF_04067};
DE AltName: Full=Non-structural protein 2 {ECO:0000255|HAMAP-Rule:MF_04067};
DE Short=NS2 {ECO:0000255|HAMAP-Rule:MF_04067};
GN Name=NS {ECO:0000255|HAMAP-Rule:MF_04067};
OS Influenza C virus (strain C/California/1978).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX NCBI_TaxID=203224;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=4032535; DOI=10.1128/jvi.56.1.221-226.1985;
RA Nakada S., Graves P.N., Desselberger U., Creager R.S., Krystal M.,
RA Palese P.;
RT "Influenza C virus RNA 7 codes for a nonstructural protein.";
RL J. Virol. 56:221-226(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=15310173; DOI=10.1017/s0950268804002146;
RA Matsuzaki Y., Takao S., Shimada S., Mizuta K., Sugawara K., Takashita E.,
RA Muraki Y., Hongo S., Nishimura H.;
RT "Characterization of antigenically and genetically similar influenza C
RT viruses isolated in Japan during the 1999-2000 season.";
RL Epidemiol. Infect. 132:709-720(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=2943090; DOI=10.1016/0168-1702(86)90005-5;
RA Nakada S., Graves P.N., Palese P.;
RT "The influenza C virus NS gene: evidence for a spliced mRNA and a second NS
RT gene product (NS2 protein).";
RL Virus Res. 4:263-273(1986).
RN [4]
RP INTERACTION WITH HUMAN XPO1/CRM1, NUCLEAR EXPORT SIGNALS, AND MUTAGENESIS
RP OF MET-100; LEU-103; LEU-105; LEU-127; LEU-130 AND LEU-132.
RX PubMed=11462009; DOI=10.1128/jvi.75.16.7375-7383.2001;
RA Paragas J., Talon J., O'Neill R.E., Anderson D.K., Garcia-Sastre A.,
RA Palese P.;
RT "Influenza B and C virus NEP (NS2) proteins possess nuclear export
RT activities.";
RL J. Virol. 75:7375-7383(2001).
CC -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC complexes and the nuclear export machinery of the cell. Possesses no
CC intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC domain. This domain is believed to allow recognition of RNPs bound to
CC the protein M1. Since protein M1 is not available in large quantities
CC before late stages of infection, such an indirect recognition mechanism
CC probably ensures that genomic RNPs are not exported from the host
CC nucleus until sufficient quantities of viral mRNA and progeny genomic
CC RNA have been synthesized. Furthermore, the RNPs enter the host
CC cytoplasm only when associated with the M1 protein that is necessary to
CC guide them to the plasma membrane. May down-regulate viral RNA
CC synthesis when overproduced. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC -!- SUBUNIT: Interacts with protein M1. May interact with host nucleoporin
CC RAB/HRB and exportin XPO1/CRM1. {ECO:0000255|HAMAP-Rule:MF_04067}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04067}. Host
CC nucleus {ECO:0000255|HAMAP-Rule:MF_04067}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=NEP; Synonyms=NS2;
CC IsoId=P33493-1; Sequence=Displayed;
CC Name=NS1;
CC IsoId=P06944-1; Sequence=External;
CC -!- SIMILARITY: Belongs to the influenza viruses NEP family.
CC {ECO:0000255|HAMAP-Rule:MF_04067}.
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DR EMBL; M10087; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR EMBL; AB099625; BAD37132.1; -; Genomic_RNA.
DR PIR; S28883; S28883.
DR SMR; P33493; -.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR GO; GO:0039675; P:exit of virus from host cell nucleus through nuclear pore; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04067; INFV_NEP; 1.
DR InterPro; IPR005188; Flu_C_NS2.
DR InterPro; IPR000968; Flu_NS2.
DR Pfam; PF03555; Flu_C_NS2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW Virion.
FT CHAIN 1..182
FT /note="Nuclear export protein"
FT /id="PRO_0000079018"
FT MOTIF 96..105
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT MOTIF 122..132
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04067"
FT MUTAGEN 100
FT /note="M->A: 90% loss of nuclear export; when associated
FT with A-103 and A-105."
FT /evidence="ECO:0000269|PubMed:11462009"
FT MUTAGEN 103
FT /note="L->A: 90% loss of nuclear export; when associated
FT with A-100 and A-105."
FT /evidence="ECO:0000269|PubMed:11462009"
FT MUTAGEN 105
FT /note="L->A: 90% loss of nuclear export; when associated
FT with A-100 and A-103."
FT /evidence="ECO:0000269|PubMed:11462009"
FT MUTAGEN 127
FT /note="L->A: 90% loss of nuclear export; when associated
FT with A-130 and A-132."
FT /evidence="ECO:0000269|PubMed:11462009"
FT MUTAGEN 130
FT /note="L->A: 90% loss of nuclear export; when associated
FT with A-127 and A-132."
FT /evidence="ECO:0000269|PubMed:11462009"
FT MUTAGEN 132
FT /note="L->A: 90% loss of nuclear export; when associated
FT with A-127 and A-130."
FT /evidence="ECO:0000269|PubMed:11462009"
SQ SEQUENCE 182 AA; 20837 MW; E074374BCC6E4142 CRC64;
MSDKTVKSTN LMAFVATKML ERQEDLDTCT EMQVEKMKTS TKARLRTESS FAPRTWEDAI
KDEILRRSVD TSSLNKWPEL KQELENVSDA LKADSLWLPM KSLSLYSRVS NQEPSSIPIG
EMKHQILTRL KLICSRLEKL DLNLSKAVLG IQNSEDLILI IYNRDICKNT ILMIKSLCNS
LI
