ID   NEP_INCGL               Reviewed;         177 AA.
AC   P0C135; P06822;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   23-FEB-2022, entry version 41.
DE   RecName: Full=Nuclear export protein;
DE            Short=NEP;
DE   AltName: Full=Non-structural protein 2;
DE            Short=NS2;
DE   Flags: Fragment;
GN   Name=NS;
OS   Influenza C virus (strain C/Great lakes/1167/1954).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX   NCBI_TaxID=11557;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2943076; DOI=10.1016/0042-6822(86)90003-6;
RA   Buonagurio D.A., Nakada S., Fitch W.M., Palese P.;
RT   "Epidemiology of influenza C virus in man: multiple evolutionary lineages
RT   and low rate of change.";
RL   Virology 153:12-21(1986).
RN   [2]
RP   IDENTIFICATION OF FRAMESHIFT.
RX   PubMed=10900030; DOI=10.1099/0022-1317-81-8-1933;
RA   Alamgir A.S.M., Matsuzaki Y., Hongo S., Tsuchiya E., Sugawara K.,
RA   Muraki Y., Nakamura K.;
RT   "Phylogenetic analysis of influenza C virus nonstructural (NS) protein
RT   genes and identification of the NS2 protein.";
RL   J. Gen. Virol. 81:1933-1940(2000).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs to which
CC       the M1 protein is bound. Because the M1 protein is not available in
CC       large quantities until the later stages of infection, such an indirect
CC       recognition mechanism probably ensures that genomic RNPs are not
CC       exported from the nucleus before sufficient quantities of viral mRNA
CC       and progeny genomic RNA have been synthesized. Furthermore, the RNPs
CC       enters the cytoplasm only when they have associated with the M1 protein
CC       that is necessary to guide them to the plasma membrane. May down-
CC       regulate viral RNA synthesis when overproduced (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Binds M1 protein. May interact with human nucleoporins and
CC       exportin XPO1/CRM1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=P0C135-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=P0C134-1; Sequence=External;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA24037.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; D00030; BAA24037.1; ALT_FRAME; Genomic_RNA.
DR   SMR; P0C135; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR005188; Flu_C_NS2.
DR   Pfam; PF03555; Flu_C_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           1..177
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000223677"
FT   MOTIF           91..100
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   MOTIF           117..127
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   177 AA;  20250 MW;  178F2C95B85472B0 CRC64;
     VKSTNLMAFV ATKMLERQED LDTCTEMQVE KMKTSTKARL RTESSFAPRT WEDAIKDEIL
     RRSVDTSSLD KWPELKQELE NVSDALKADS LWLPMKSLSL YSKVSNQELS SIPIGEMKHQ
     ILTRLKLICS RLEKLDLNLS KAVLGIQNSE DLILIIYNRD VCKNTILMIK SLCNSLI