ID   NEP_INCJJ               Reviewed;         122 AA.
AC   P12603;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 2.
DT   29-SEP-2021, entry version 66.
DE   RecName: Full=Nuclear export protein;
DE            Short=NEP;
DE   AltName: Full=Non-structural protein 2;
DE            Short=NS2;
DE   Flags: Fragment;
GN   Name=NS;
OS   Influenza C virus (strain C/JJ/1950).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Gammainfluenzavirus.
OX   NCBI_TaxID=11560;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2943090; DOI=10.1016/0168-1702(86)90005-5;
RA   Nakada S., Graves P.N., Palese P.;
RT   "The influenza C virus NS gene: evidence for a spliced mRNA and a second NS
RT   gene product (NS2 protein).";
RL   Virus Res. 4:263-273(1986).
CC   -!- FUNCTION: Mediates the nuclear export of encapsidated genomic RNAs
CC       (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs
CC       complexes and the nuclear export machinery of the cell. Possesses no
CC       intrinsic RNA-binding activity, but includes a C-terminal M1-binding
CC       domain. This domain is believed to allow recognition of RNPs to which
CC       the M1 protein is bound. Because the M1 protein is not available in
CC       large quantities until the later stages of infection, such an indirect
CC       recognition mechanism probably ensures that genomic RNPs are not
CC       exported from the nucleus before sufficient quantities of viral mRNA
CC       and progeny genomic RNA have been synthesized. Furthermore, the RNPs
CC       enters the cytoplasm only when they have associated with the M1 protein
CC       that is necessary to guide them to the plasma membrane. May down-
CC       regulate viral RNA synthesis when overproduced (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Binds M1 protein. May interact with human nucleoporins and
CC       exportin XPO1/CRM1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=NEP; Synonyms=NS2;
CC         IsoId=P12603-1; Sequence=Displayed;
CC       Name=NS1;
CC         IsoId=P12603-2; Sequence=Not described;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA43796.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M22015; AAA43796.1; ALT_FRAME; Genomic_RNA.
DR   SMR; P12603; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR005188; Flu_C_NS2.
DR   Pfam; PF03555; Flu_C_NS2; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Host nucleus; Host-virus interaction; Transport;
KW   Virion.
FT   CHAIN           1..122
FT                   /note="Nuclear export protein"
FT                   /id="PRO_0000079019"
FT   MOTIF           96..105
FT                   /note="Nuclear export signal"
FT                   /evidence="ECO:0000250"
FT   NON_TER         122
SQ   SEQUENCE   122 AA;  13936 MW;  1A413AE46A549CCD CRC64;
     MSDKTVKSTN LMAFVATKML ERQEDLDTCT EMQVEKMKTS TKARLKTESS FAPRTWEDAI
     KDKILRRSVD TSSLDKWQEL KQELENVSDA LKADSLWLPM KSLSLYSKVS NQEPSSIPIG
     EM