ID   NER_BPD10               Reviewed;          73 AA.
AC   P06903; C9DGL0;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   29-SEP-2021, entry version 99.
DE   RecName: Full=Negative regulator of transcription;
DE            Short=Ner;
GN   Name=ner;
OS   Escherichia phage D108 (Bacteriophage D108).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Muvirus; unclassified Muvirus.
OX   NCBI_TaxID=665033;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2998774; DOI=10.1002/j.1460-2075.1985.tb04040.x;
RA   Tolias P.P., Dubow M.S.;
RT   "The cloning and characterization of the bacteriophage D108 regulatory DNA-
RT   binding protein ner.";
RL   EMBO J. 4:3031-3037(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3012481; DOI=10.1093/nar/14.9.3813;
RA   Mizuuchi M., Weisberg R.A., Mizuuchi K.;
RT   "DNA sequence of the control region of phage D108: the N-terminal amino
RT   acid sequences of repressor and transposase are similar both in phage D108
RT   and in its relative, phage Mu.";
RL   Nucleic Acids Res. 14:3813-3825(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kropinski A.M., Villegas A., Lingohr E.J.;
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Switches off the PcM promoter thereby blocking expression of
CC       the major repressor Repc (latency factor) and favoring transcription
CC       from the early promoter (Pe) and hence viral transposition and lytic
CC       development. Negatively regulates the early promoter (Pe) thereby
CC       controlling its own expression and keeping the level of early genes
CC       expression independent of the viral genome copy number (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       Early gene transcription is repressed by viral Repc (latency) and
CC       favored by viral Ner protein (lytic development).
CC   -!- SIMILARITY: Belongs to the ner transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; M26291; AAA32206.1; -; Genomic_DNA.
DR   EMBL; X03847; CAA27475.1; -; Genomic_DNA.
DR   EMBL; GQ357916; ACV50261.1; -; Genomic_DNA.
DR   PIR; A24680; DNBPD8.
DR   RefSeq; YP_003335750.1; NC_013594.1.
DR   SMR; P06903; -.
DR   GeneID; 8658813; -.
DR   KEGG; vg:8658813; -.
DR   Proteomes; UP000000320; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR038722; Ner_HTH_dom.
DR   Pfam; PF13693; HTH_35; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Early protein; Host cytoplasm; Pyruvate; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..73
FT                   /note="Negative regulator of transcription"
FT                   /id="PRO_0000062783"
FT   DNA_BIND        28..47
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   73 AA;  8534 MW;  898DFD38E1409025 CRC64;
     MHMNKRTNRQ DWHRADIVAE LRKRNMSLAE LGRSNHLSSS TLKNALDKRY PKAEKIIADA
     LGMTPQDIWP SRY