NES1_FRAVE
ID NES1_FRAVE Reviewed; 580 AA.
AC P0CV96;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=(3S,6E)-nerolidol synthase 1, chloroplastic;
DE Short=FvNES1;
DE EC=4.2.3.48;
DE Flags: Precursor;
OS Fragaria vesca (Woodland strawberry) (Potentilla vesca).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC Fragaria.
OX NCBI_TaxID=57918;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA Aharoni A., Jongsma M.A., Verhoeven H.A., Bouwmeester H.J.;
RT "Isoprenoid synthases.";
RL Patent number WO02064764, 22-AUG-2002.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF TRP-6 AND ILE-16,
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=15522848; DOI=10.1105/tpc.104.023895;
RA Aharoni A., Giri A.P., Verstappen F.W., Bertea C.M., Sevenier R., Sun Z.,
RA Jongsma M.A., Schwab W., Bouwmeester H.J.;
RT "Gain and loss of fruit flavor compounds produced by wild and cultivated
RT strawberry species.";
RL Plant Cell 16:3110-3131(2004).
CC -!- FUNCTION: Involved in monoterpene (C10) and sesquiterpene (C15)
CC biosynthesis. Converts geranyl diphosphate (GPP) into S-linalool and
CC farnesyl diphosphate (FPP) into (3S)-E-nerolidol (By similarity).
CC Probably not expressed in wild strawberry species. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O = (3S,6E)-nerolidol +
CC diphosphate; Xref=Rhea:RHEA:27530, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:59958, ChEBI:CHEBI:175763;
CC EC=4.2.3.48;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:15522848}.
CC -!- DEVELOPMENTAL STAGE: Not expressed in red stage fruit tissue.
CC {ECO:0000269|PubMed:15522848}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsg subfamily.
CC {ECO:0000305}.
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DR EMBL; AX529002; CAD57084.1; -; Unassigned_DNA.
DR AlphaFoldDB; P0CV96; -.
DR SMR; P0CV96; -.
DR UniPathway; UPA00213; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Lyase; Magnesium; Manganese; Metal-binding; Plastid;
KW Transit peptide.
FT TRANSIT 1..31
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 32..580
FT /note="(3S,6E)-nerolidol synthase 1, chloroplastic"
FT /id="PRO_0000407982"
FT MOTIF 334..338
FT /note="DDXXD motif"
FT BINDING 334
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 334
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 338
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 338
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 478
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 482
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 486
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT MUTAGEN 6
FT /note="W->R: Dual targeting to mitochondrion and
FT chloroplast; when associated with I-16 deletion."
FT /evidence="ECO:0000269|PubMed:15522848"
FT MUTAGEN 16
FT /note="Missing: Dual targeting to mitochondrion and
FT chloroplast; when associated with R-6."
FT /evidence="ECO:0000269|PubMed:15522848"
SQ SEQUENCE 580 AA; 66532 MW; 557B6FFE25C61391 CRC64;
MASSSWAFFK VFNPQIAPKS ISHIGQSDLM QLTHKKQLPT FQRRGIAEDS LLPSSTTPIK
PMHVETKHTR TMGDIFVQHS QKLELFRNVL RNAAELDALE GLNMIDAVQR LGIDYHFQRE
IDEILHKQMG IVSACDDLYE VALRFRLLRQ HGYFVPEDVF NNFKDSKGTF KQVLGEDIKG
LMSLYEASQL GTEGEDTLVE AEKFSGHLLK TSLSHLDRHR ARIVGNTLRN PHRKSLASFM
ARNFFVTSQA TNSWLNLLKE VAKTDFNMVR SVHQKEIVQI SKWWKELGLV KELKFARDQP
LKWYTWSMAG LTDPKLSEER VELTKPISFV YLIDDIFDVY GTLDDLILFT EAVNRWEITA
IDHLPDYMKI CFKALYDMTN EFSCKVYQKH GWNPLRSLKI SWASLCNAFL VEAKWFASGQ
LPKSEEYLKN GIVSSGVNVG LVHMFFLLGQ NITRKSVELL NETPAMISSS AAILRLWDDL
GSAKDENQDG NDGSYVRCYL EEHEGCSIEE AREKTINMIS DEWKKLNREL LSPNPFPATF
TSASLNLARM IPLMYSYDGN QSLPSLKEYM KLMLYETVSM
