NESD_DROME
ID NESD_DROME Reviewed; 602 AA.
AC Q9VIP0; Q6NP13;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein nessun dorma {ECO:0000312|FlyBase:FBgn0032848};
GN Name=nesd {ECO:0000312|FlyBase:FBgn0032848};
GN ORFNames=CG10722 {ECO:0000312|FlyBase:FBgn0032848};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAR82785.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAR82785.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH PAV AND TUM, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DISRUPTION PHENOTYPE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=21187330; DOI=10.1083/jcb.201007060;
RA Montembault E., Zhang W., Przewloka M.R., Archambault V., Sevin E.W.,
RA Laue E.D., Glover D.M., D'Avino P.P.;
RT "Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis
RT in Drosophila spermatocytes.";
RL J. Cell Biol. 191:1351-1365(2010).
CC -!- FUNCTION: Required during male meiosis for completion of spermatocyte
CC cytokinesis and possibly also required in female germline cells. Also
CC involved in ring canal formation in male and female germline cells. Not
CC essential for cleavage furrow ingression but is required for
CC contractile ring stability and the attachment of the furrowing membrane
CC to the actomyosin ring in late telophase. Displays high binding
CC affinity for beta-galactosides. {ECO:0000269|PubMed:21187330}.
CC -!- SUBUNIT: Interacts (via N-terminus) with both members of the
CC centralspindlin complex, Pav and Tum. {ECO:0000269|PubMed:21187330}.
CC -!- SUBCELLULAR LOCATION: Midbody {ECO:0000269|PubMed:21187330}.
CC Note=Detected in the midbody in late cytokinesis. Also detected in ring
CC canals in testis and ovary and in the fusome.
CC {ECO:0000269|PubMed:21187330}.
CC -!- TISSUE SPECIFICITY: Detected in testis (at protein level). Also
CC expressed in ovary. {ECO:0000269|PubMed:21187330}.
CC -!- DISRUPTION PHENOTYPE: Some mutants display multinucleate spermatids,
CC sterility and asymmetric contraction of the contractile ring with
CC subsequent collapse of the cleavage furrow.
CC {ECO:0000269|PubMed:21187330}.
CC -!- MISCELLANEOUS: The name 'nessun dorma' is based on Pavarotti's famous
CC aria from the opera Turandot due to the interaction of nesd with Pav.
CC {ECO:0000303|PubMed:21187330}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR82785.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAF53876.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN11067.1; -; Genomic_DNA.
DR EMBL; BT011118; AAR82785.1; ALT_INIT; mRNA.
DR RefSeq; NP_610025.1; NM_136181.2.
DR RefSeq; NP_724247.1; NM_165320.2.
DR AlphaFoldDB; Q9VIP0; -.
DR SMR; Q9VIP0; -.
DR IntAct; Q9VIP0; 2.
DR STRING; 7227.FBpp0080867; -.
DR PaxDb; Q9VIP0; -.
DR PRIDE; Q9VIP0; -.
DR DNASU; 35298; -.
DR EnsemblMetazoa; FBtr0081334; FBpp0080866; FBgn0032848.
DR EnsemblMetazoa; FBtr0081335; FBpp0080867; FBgn0032848.
DR GeneID; 35298; -.
DR KEGG; dme:Dmel_CG10722; -.
DR UCSC; CG10722-RA; d. melanogaster.
DR CTD; 35298; -.
DR FlyBase; FBgn0032848; nesd.
DR VEuPathDB; VectorBase:FBgn0032848; -.
DR eggNOG; ENOG502QUQ2; Eukaryota.
DR GeneTree; ENSGT00940000166163; -.
DR HOGENOM; CLU_022717_1_0_1; -.
DR InParanoid; Q9VIP0; -.
DR OMA; KIEPAGW; -.
DR OrthoDB; 1276823at2759; -.
DR PhylomeDB; Q9VIP0; -.
DR SignaLink; Q9VIP0; -.
DR BioGRID-ORCS; 35298; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35298; -.
DR PRO; PR:Q9VIP0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032848; Expressed in eye disc (Drosophila) and 16 other tissues.
DR GO; GO:0097149; C:centralspindlin complex; IDA:FlyBase.
DR GO; GO:0035324; C:female germline ring canal; IDA:FlyBase.
DR GO; GO:0045169; C:fusome; IDA:FlyBase.
DR GO; GO:0045171; C:intercellular bridge; IDA:FlyBase.
DR GO; GO:0035323; C:male germline ring canal; IDA:FlyBase.
DR GO; GO:0030496; C:midbody; IDA:FlyBase.
DR GO; GO:0030246; F:carbohydrate binding; IDA:FlyBase.
DR GO; GO:0008335; P:female germline ring canal stabilization; IMP:FlyBase.
DR GO; GO:0007112; P:male meiosis cytokinesis; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR045140; SHCBP1-like.
DR PANTHER; PTHR14695; PTHR14695; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Coiled coil; Meiosis; Reference proteome.
FT CHAIN 1..602
FT /note="Protein nessun dorma"
FT /id="PRO_0000437209"
FT COILED 188..208
FT /evidence="ECO:0000255"
SQ SEQUENCE 602 AA; 67728 MW; 85986E26E0281E0D CRC64;
MEVFTFEKSY LERLKEAEAV LSWEGAVMPA SQVRSEWKSY VELKIEPAGW QAIWKIPRVI
CEDLKLRYPT IVYGYVEQVI FDELKAVFVV TAVQDSDVHL PESNEVSLVE LWPTVQQENA
ALNVDTTAEC IDRLRFFYTH VWMPWDKDYD DDRDWVQQHL QARIQLVCDL SKNRLPRPLA
LHMRTLLAEA KYIQQRLDYL ELDLSDAESD DEAVELNDSV AEPARKQSKA GSANGCLNVS
SLPVTDLMCL HLRMAIIRSE FEILENPEMR RAYSELQSNS LKRLRCSSGR TRQSEDLLVE
RNSISHVVTL PGKLQQQLEL LKLAQTLVKP ESKVQLSNTL QDVLSICQSN DDILLSPGEH
TIKFLEHLND NGSLRGLTQP EAILSPAADL SLLPVVCSSD EDSTLLVIDG DYTLSELVLD
CRHVRRGILL RNGTLTMRGC RLLGDGSSST QEGIVCMPGA SVELKSCLIE NFAVGVSMRP
KSSAELGSVQ FKTCKTGLEL LEKSASVNLQ GSKCSFENCA LGILADGFVL GEQRTEKVLV
LNKFSELQRY NEDNLLGNCS FYNCKKNVRV FNESGQLLAQ RSHQQLLEDE LGGENKENIQ
LV
