NEST_DANRE
ID NEST_DANRE Reviewed; 1265 AA.
AC P86839;
DT 08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Nestin;
GN Name=nes;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305}
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=AB {ECO:0000269|PubMed:17651502};
RX PubMed=17651502; DOI=10.1186/1471-213x-7-89;
RA Mahler J., Driever W.;
RT "Expression of the zebrafish intermediate neurofilament Nestin in the
RT developing nervous system and in neural proliferation zones at
RT postembryonic stages.";
RL BMC Dev. Biol. 7:89-89(2007).
RN [3] {ECO:0000305}
RP FUNCTION.
RC STRAIN=AB {ECO:0000269|PubMed:20174467};
RX PubMed=20174467; DOI=10.1371/journal.pone.0009318;
RA Chen H.L., Yuh C.H., Wu K.K.;
RT "Nestin is essential for zebrafish brain and eye development through
RT control of progenitor cell apoptosis.";
RL PLoS ONE 5:E9318-E9318(2010).
CC -!- FUNCTION: Promotes the disassembly of phosphorylated vimentin
CC intermediate filaments (IF) during mitosis and may play a role in the
CC trafficking and distribution of IF proteins and other cellular factors
CC to daughter cells during progenitor cell division. Required for
CC survival, renewal and mitogen-stimulated proliferation of neural
CC progenitor cells (By similarity). Required for brain and eye
CC development. {ECO:0000250, ECO:0000269|PubMed:20174467}.
CC -!- SUBUNIT: Forms homodimers and homotetramers in vitro. In mixtures with
CC other intermediate filament proteins such as vimentin and alpha-
CC internexin, preferentially forms heterodimers (By similarity).
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed throughout the developing nervous
CC system at 24 hours post-fertilization (hpf). As development progresses,
CC expression becomes restricted to proliferative zones of the developing
CC and postembryonic central nervous system. In the peripheral nervous
CC system, expressed in the cranial ganglia. Also expressed in mesodermal
CC muscle precursor cells and in cranial mesenchymal tissue.
CC {ECO:0000269|PubMed:17651502}.
CC -!- DEVELOPMENTAL STAGE: Expression is not detected before the 3-somite
CC stage at 10-11 hours post-fertilization. Earliest expression is
CC detected during mid-neurulation. {ECO:0000269|PubMed:17651502}.
CC -!- SIMILARITY: Belongs to the intermediate filament family.
CC {ECO:0000255|PROSITE-ProRule:PRU01188}.
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DR EMBL; CR848841; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_001919922.1; XM_001919887.6.
DR AlphaFoldDB; P86839; -.
DR SMR; P86839; -.
DR STRING; 7955.ENSDARP00000112046; -.
DR PaxDb; P86839; -.
DR Ensembl; ENSDART00000122681; ENSDARP00000112046; ENSDARG00000088805.
DR GeneID; 100150939; -.
DR KEGG; dre:100150939; -.
DR CTD; 10763; -.
DR ZFIN; ZDB-GENE-071119-2; nes.
DR eggNOG; ENOG502RYFK; Eukaryota.
DR GeneTree; ENSGT00940000167106; -.
DR HOGENOM; CLU_274633_0_0_1; -.
DR InParanoid; P86839; -.
DR OMA; TQASHMD; -.
DR OrthoDB; 78144at2759; -.
DR PhylomeDB; P86839; -.
DR PRO; PR:P86839; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 16.
DR Bgee; ENSDARG00000088805; Expressed in pretectal region and 46 other tissues.
DR GO; GO:0005882; C:intermediate filament; IBA:GO_Central.
DR GO; GO:0031730; F:CCR5 chemokine receptor binding; IBA:GO_Central.
DR GO; GO:0019215; F:intermediate filament binding; ISS:UniProtKB.
DR GO; GO:0007420; P:brain development; IMP:UniProtKB.
DR GO; GO:0021545; P:cranial nerve development; IMP:ZFIN.
DR GO; GO:0031076; P:embryonic camera-type eye development; IMP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0030844; P:positive regulation of intermediate filament depolymerization; ISS:UniProtKB.
DR GO; GO:2000179; P:positive regulation of neural precursor cell proliferation; ISS:UniProtKB.
DR InterPro; IPR018039; IF_conserved.
DR InterPro; IPR039008; IF_rod_dom.
DR InterPro; IPR031211; Nestin.
DR PANTHER; PTHR47051; PTHR47051; 2.
DR Pfam; PF00038; Filament; 1.
DR SMART; SM01391; Filament; 1.
DR PROSITE; PS00226; IF_ROD_1; 1.
DR PROSITE; PS51842; IF_ROD_2; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Developmental protein; Intermediate filament; Neurogenesis;
KW Reference proteome.
FT CHAIN 1..1265
FT /note="Nestin"
FT /id="PRO_0000404197"
FT DOMAIN 17..324
FT /note="IF rod"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01188"
FT REGION 1..16
FT /note="Head"
FT /evidence="ECO:0000255"
FT REGION 17..52
FT /note="Coil 1A"
FT /evidence="ECO:0000255"
FT REGION 53..64
FT /note="Linker 1"
FT /evidence="ECO:0000255"
FT REGION 65..160
FT /note="Coil 1B"
FT /evidence="ECO:0000255"
FT REGION 161..183
FT /note="Linker 12"
FT /evidence="ECO:0000255"
FT REGION 184..203
FT /note="Coil 2A"
FT /evidence="ECO:0000255"
FT REGION 204..206
FT /note="Linker 2"
FT /evidence="ECO:0000255"
FT REGION 207..324
FT /note="Coil 2B"
FT /evidence="ECO:0000255"
FT REGION 325..1265
FT /note="Tail"
FT /evidence="ECO:0000255"
FT REGION 383..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 667..830
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 863..1073
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1093..1116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1232..1265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 404..422
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..441
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 447..475
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 476..499
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 674..698
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 715..729
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 778..830
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 865..901
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 909..933
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 934..954
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 959..976
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 977..1048
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1057..1073
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1242..1256
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1265 AA; 143400 MW; 28F7142CCB4A2715 CRC64;
MELLGVRQPF GPQFQEEKYQ MLELNHRLES YLGRVKLLEE ENKLLREEIH TLKSSRDPAG
QRKAQEEALS QARRMMEEAW RKKDCVELEV ENLMEDMEKV SFQRKKVKTA QAEAQRKLTE
SRKELEEERR AQIWLREKVG QLEKDLMLQM QVHQENMETM QASLKQTKQV LMAPQRVQAT
SIPDLGQEYG YKAAQAWQEA ANNYQKQVGR LEESLNQTKA NMTKIYQEKR ESQRQVQHLA
KELESTRIKR QMLEKHAMQQ REEQKEELQH LQAQVNTLEL EKDCLGQQID SLMLDRQHLL
QVKMSLGLEV ATYRALLDSE GLRIDRPTPN KTSSTVFLDA LSKPTGIQST SQTTAASCLL
SSSVSTSHRS IASSRSLLTS AAPSWTLTRG TPQRPPSSTS MTEKTEDHIS EETKRSAEES
VDQLQQEKVQ QDWTLESTLP KTSAEPKPEL QPEEIKVEDE ANEPQQAHMV ESKTDESELT
SVPAEQQGSM SQTPETKSWA GHFNDPAEVS EDGKDEDTEV SVEMARISHA PKVAWEEIKS
AVEDEKDDAS EMDVRSEIIS ESHTFAYGDA ENDSNTLTSS HISQNTNALA SSFLEQGTLD
SASYFGYEAT NENLMKEEEL DNVSNISEEV TEQLNSETEA AIDSINEWDR QEESEPENET
KVMTSYFEVE EGEMSINTDT KDKTEDDIER EELEVTEREI LVQSAGGMLG VDLPNDTDHQ
DEHNLPQIEL NEDQSEAEEE NQKEKQCDED DSPNISASLK TDPGEGDSYS QEHTLADTRP
LIRYKSDEET SHHIGETSDS EDEKERIDQG HLNEKRFNTM EDLTEEPDME VTGKMRLEDL
VSNEEAAADD VASVMFQKVS GDHESLDVVV QESERKGNLE KEDSGDADNM RDNKEEDVSV
FEQNENQQLT EHQHVHTEQV EDKPVHSHET QEHVNTEFSS TFSERSQQEQ LEESSLTMFE
DEAATKEAED SDVSMHTNID LIDSRSLESE INGQPDIMTS DMANSECNSS EDESPNASQC
FQNTSLLAAA TPNEQPLTFT NEVSKADYVS DNNDVPEEVL SEEKSTDEPK ASQIDDLENF
NIWGESTSIS DAAQTTHASM DEHSSISPVN ENLESSNKIP SVAENIFGHF EEHLERSVES
FPEKEVTVMD FENNDLGEEF QSKQMKSEIH SFFSTSLKQD FWSEGKMEMA ATYDPAKTED
LNQAMVFGEE WREIGVQSAN GDTKEEMEIL KIRDDKQKDG QPAQSKIVQS DDSADEGDSW
SSGDE
