NET2A_ARATH
ID NET2A_ARATH Reviewed; 947 AA.
AC P0DMS1; Q9C6Q9;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 04-FEB-2015, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Protein NETWORKED 2A {ECO:0000303|PubMed:22840520};
GN Name=NET2A {ECO:0000303|PubMed:22840520};
GN OrderedLocusNames=At1g58215 {ECO:0000312|Araport:AT1G58215};
GN ORFNames=T18I24.12 {ECO:0000312|EMBL:AAG50760.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DOMAIN, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY,
RP AND NOMENCLATURE.
RX PubMed=22840520; DOI=10.1016/j.cub.2012.06.041;
RA Deeks M.J., Calcutt J.R., Ingle E.K., Hawkins T.J., Chapman S.,
RA Richardson A.C., Mentlak D.A., Dixon M.R., Cartwright F., Smertenko A.P.,
RA Oparka K., Hussey P.J.;
RT "A superfamily of actin-binding proteins at the actin-membrane nexus of
RT higher plants.";
RL Curr. Biol. 22:1595-1600(2012).
RN [4]
RP GENE FAMILY.
RX PubMed=24926301; DOI=10.3389/fpls.2014.00254;
RA Hawkins T.J., Deeks M.J., Wang P., Hussey P.J.;
RT "The evolution of the actin binding NET superfamily.";
RL Front. Plant Sci. 5:254-254(2014).
CC -!- FUNCTION: Plant-specific actin binding protein. Associates with F-actin
CC at the plasma membrane in growing pollen tubes. May be part of a
CC membrane-cytoskeletal adapter complex. {ECO:0000269|PubMed:22840520}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22840520}.
CC Note=In growing pollen tubes, forms foci at the plasma membrane, but
CC not at the pollen tube tip. {ECO:0000269|PubMed:22840520}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in pollen.
CC {ECO:0000269|PubMed:22840520}.
CC -!- DOMAIN: The NAB domain, also called NAB (NET actin-binding) domain, is
CC sufficient for F-actin binding. {ECO:0000269|PubMed:22840520}.
CC -!- SIMILARITY: Belongs to the NET family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50760.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g58210 has been split into 2 genes: At1g58210 and At1g58215.; Evidence={ECO:0000305};
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DR EMBL; AC079131; AAG50760.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; ANM59275.1; -; Genomic_DNA.
DR RefSeq; NP_001321645.1; NM_001333822.1.
DR AlphaFoldDB; P0DMS1; -.
DR SMR; P0DMS1; -.
DR STRING; 3702.AT1G58210.1; -.
DR PaxDb; P0DMS1; -.
DR PRIDE; P0DMS1; -.
DR ProteomicsDB; 250589; -.
DR EnsemblPlants; AT1G58215.1; AT1G58215.1; AT1G58215.
DR GeneID; 842188; -.
DR Gramene; AT1G58215.1; AT1G58215.1; AT1G58215.
DR KEGG; ath:AT1G58215; -.
DR Araport; AT1G58215; -.
DR eggNOG; ENOG502QQVH; Eukaryota.
DR OMA; HEMNGLK; -.
DR OrthoDB; 186795at2759; -.
DR PRO; PR:P0DMS1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P0DMS1; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0051015; F:actin filament binding; IDA:UniProtKB.
DR InterPro; IPR011684; NAB.
DR Pfam; PF07765; KIP1; 1.
DR PROSITE; PS51774; NAB; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Coiled coil; Membrane; Reference proteome.
FT CHAIN 1..947
FT /note="Protein NETWORKED 2A"
FT /id="PRO_0000431853"
FT DOMAIN 10..90
FT /note="NAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01110"
FT REGION 105..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 618..675
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 743..763
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 911..947
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 348..454
FT /evidence="ECO:0000255"
FT COILED 568..619
FT /evidence="ECO:0000255"
FT COILED 722..809
FT /evidence="ECO:0000255"
FT COMPBIAS 620..643
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 644..659
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 749..763
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 911..936
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 947 AA; 108032 MW; 56BB435AE3119025 CRC64;
MLQRAASNAY SWWWASHIRT KQSKWLEHNL QDMEEKVEYT LKIIDEDGDT FAKRAEMYYR
KRPEIVNFVE EAFRSYRALA ERYDHLSREL QSANRTIATA FPEHVQFPLE DDSDENEDYD
GRPRKPPKHL HLIPKGINIP EVPDIPKKKD FRSQSMMLSR KGPADLKRNV SSAQAKREAA
IVRSGLSKEE GLEEIDKLQK GILALQTEKE FVRSSYEESY ERYWDLENEV TEMQKSVCNL
QDEFGLGASI DDSDARTLMA STALSSCRDT LAKLEEKQKI SIEEAEIEKG RITTAKERFY
ALRNKFEKPE SDVLDEVIRT DEEEEDVVQE SSYESEREDS NENLTVVKLA EKIDDLVHRV
VSLETNASSH TALVKTLRSE TDELHEHIRG LEEDKAALVS DATVMKQRIT VLEDELRNVR
KLFQKVEDQN KNLQNQFKVA NRTVDDLSGK IQDVKMDEDV EGAGIFQELP VVSGSEDSRD
DLKSVSTEKT KKDVIAVKES EDGERAQEEK PEIKDSFALS ETASTCFGTE AEDLVTEDED
EETPNWRHLL PDGMEDREKV LLDEYTSVLR DYREVKRKLG DVEKKNREGF FELALQLREL
KNAVAYKDVE IQSLRQKLDT TGKDSPHQGE GNNQLEHEQG HHETVSISPT SNFSVATTPH
HQVGDVKRTP GRTKSTEVRV KFADVDDSPR TKIPTVEDKV RADIDAVLEE NLEFWLRFST
SVHQIQKYQT TVQDLKSELS KLRIESKQQQ ESPRSSSNTA VASEAKPIYR HLREIRTELQ
LWLENSAVLK DELQGRYASL ANIQEEIARV TAQSGGNKVS DSEISGYQAA KFHGEILNMK
QENKRVSTEL HSGLDRVRAL KTEVERILSK LEEDLGISSA TEARTTPSKS SSSGRPRIPL
RSFLFGVKLK KNRQQKQSAS SLFSCVSPSP GLHKQSSYSR PPGKLPE
