NET4A_ARATH
ID NET4A_ARATH Reviewed; 558 AA.
AC F4KEW8; F4KEW9; Q0WT63; Q2V2X5; Q9LVL8;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Protein NETWORKED 4A {ECO:0000303|PubMed:22840520};
GN Name=NET4A {ECO:0000303|PubMed:22840520};
GN OrderedLocusNames=At5g58320 {ECO:0000312|Araport:AT5G58320};
GN ORFNames=MCK7.19 {ECO:0000312|EMBL:BAA96923.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DOMAIN, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY,
RP AND NOMENCLATURE.
RX PubMed=22840520; DOI=10.1016/j.cub.2012.06.041;
RA Deeks M.J., Calcutt J.R., Ingle E.K., Hawkins T.J., Chapman S.,
RA Richardson A.C., Mentlak D.A., Dixon M.R., Cartwright F., Smertenko A.P.,
RA Oparka K., Hussey P.J.;
RT "A superfamily of actin-binding proteins at the actin-membrane nexus of
RT higher plants.";
RL Curr. Biol. 22:1595-1600(2012).
RN [5]
RP GENE FAMILY.
RX PubMed=24926301; DOI=10.3389/fpls.2014.00254;
RA Hawkins T.J., Deeks M.J., Wang P., Hussey P.J.;
RT "The evolution of the actin binding NET superfamily.";
RL Front. Plant Sci. 5:254-254(2014).
CC -!- FUNCTION: Plant-specific actin binding protein. Associates with F-actin
CC at the tonoplast. May be part of a membrane-cytoskeletal adapter
CC complex. {ECO:0000269|PubMed:22840520}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:22840520}.
CC Note=Arranged into short filaments and foci.
CC {ECO:0000269|PubMed:22840520}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=F4KEW8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4KEW8-2; Sequence=VSP_057452;
CC Name=3;
CC IsoId=F4KEW8-3; Sequence=VSP_057451, VSP_057453, VSP_057455;
CC Name=4;
CC IsoId=F4KEW8-4; Sequence=VSP_057454, VSP_057456;
CC -!- TISSUE SPECIFICITY: Expressed in the epidermis of the root meristem and
CC the early expansion zone. {ECO:0000269|PubMed:22840520}.
CC -!- DOMAIN: The NAB domain, also called NAB (NET actin-binding) domain, is
CC sufficient for F-actin binding. {ECO:0000269|PubMed:22840520}.
CC -!- SIMILARITY: Belongs to the NET family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA96923.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB019228; BAA96923.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97033.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97034.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97035.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70915.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70916.1; -; Genomic_DNA.
DR EMBL; AK227698; BAE99685.1; -; mRNA.
DR RefSeq; NP_001032095.1; NM_001037018.2. [F4KEW8-3]
DR RefSeq; NP_001318828.1; NM_001345297.1. [F4KEW8-2]
DR RefSeq; NP_001332489.1; NM_001345298.1. [F4KEW8-1]
DR RefSeq; NP_200640.2; NM_125217.4. [F4KEW8-1]
DR RefSeq; NP_851213.2; NM_180882.3. [F4KEW8-2]
DR AlphaFoldDB; F4KEW8; -.
DR SMR; F4KEW8; -.
DR STRING; 3702.AT5G58320.2; -.
DR PaxDb; F4KEW8; -.
DR PRIDE; F4KEW8; -.
DR ProteomicsDB; 251295; -. [F4KEW8-1]
DR EnsemblPlants; AT5G58320.1; AT5G58320.1; AT5G58320. [F4KEW8-2]
DR EnsemblPlants; AT5G58320.2; AT5G58320.2; AT5G58320. [F4KEW8-1]
DR EnsemblPlants; AT5G58320.3; AT5G58320.3; AT5G58320. [F4KEW8-3]
DR EnsemblPlants; AT5G58320.4; AT5G58320.4; AT5G58320. [F4KEW8-2]
DR EnsemblPlants; AT5G58320.5; AT5G58320.5; AT5G58320. [F4KEW8-1]
DR GeneID; 835944; -.
DR Gramene; AT5G58320.1; AT5G58320.1; AT5G58320. [F4KEW8-2]
DR Gramene; AT5G58320.2; AT5G58320.2; AT5G58320. [F4KEW8-1]
DR Gramene; AT5G58320.3; AT5G58320.3; AT5G58320. [F4KEW8-3]
DR Gramene; AT5G58320.4; AT5G58320.4; AT5G58320. [F4KEW8-2]
DR Gramene; AT5G58320.5; AT5G58320.5; AT5G58320. [F4KEW8-1]
DR KEGG; ath:AT5G58320; -.
DR Araport; AT5G58320; -.
DR TAIR; locus:2161268; AT5G58320.
DR eggNOG; ENOG502QQTB; Eukaryota.
DR HOGENOM; CLU_032761_0_0_1; -.
DR InParanoid; F4KEW8; -.
DR OMA; TKEMPQD; -.
DR OrthoDB; 292839at2759; -.
DR PRO; PR:F4KEW8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KEW8; baseline and differential.
DR GO; GO:0005774; C:vacuolar membrane; IDA:TAIR.
DR GO; GO:0003779; F:actin binding; IEA:InterPro.
DR InterPro; IPR011684; NAB.
DR Pfam; PF07765; KIP1; 1.
DR PROSITE; PS51774; NAB; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Membrane; Reference proteome; Vacuole.
FT CHAIN 1..558
FT /note="Protein NETWORKED 4A"
FT /id="PRO_0000431860"
FT DOMAIN 21..101
FT /note="NAB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01110"
FT REGION 162..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 194..363
FT /evidence="ECO:0000255"
FT COILED 421..527
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..43
FT /note="Missing (in isoform 3)"
FT /id="VSP_057451"
FT VAR_SEQ 489..522
FT /note="SEIKARDDRTMEMEKEVEKQRRELEEVAEEKREV -> M (in isoform
FT 2)"
FT /id="VSP_057452"
FT VAR_SEQ 489..490
FT /note="SE -> RK (in isoform 3)"
FT /id="VSP_057453"
FT VAR_SEQ 490..535
FT /note="EIKARDDRTMEMEKEVEKQRRELEEVAEEKREVIRQLCFSLDYSRD -> DK
FT TALFFTRLLQRRIQETTHSLFRASTNKTIIHSCFLILVYSIYSL (in isoform
FT 4)"
FT /id="VSP_057454"
FT VAR_SEQ 491..558
FT /note="Missing (in isoform 3)"
FT /id="VSP_057455"
FT VAR_SEQ 536..558
FT /note="Missing (in isoform 4)"
FT /id="VSP_057456"
SQ SEQUENCE 558 AA; 64179 MW; 3E813163CC10D995 CRC64;
MDYDLLRSKK SIKRVESTKS NPWWWDSHIG LKNSKWLENN LDEMDRSVKR MVKLIEEDAD
SFAKKAEMYY QSRPELIALV DEFHRMYRAL AERYENITGE LRKGSPLELQ SQGSGLSDIS
ASDLSALWTS NEVNRLGRPP SGRRAPGFEY FLGNGGLPSD LYHKDGDDSA SITDSELESD
DSSVTNYPGY VSIGSDFQSL SKRIMDLEIE LREAKERLRM QLEGNTESLL PRVKSETKFV
DFPAKLAACE QELKDVNEKL QNSEDQIYIL KSQLARYLPS GLDDEQSEGA ASTQELDIET
LSEELRITSL RLREAEKQNG IMRKEVEKSK SDDAKLKSLQ DMLESAQKEA AAWKSKASAD
KREVVKLLDR ISMLKSSLAG RDHEIRDLKT ALSDAEEKIF PEKAQVKADI AKLLEEKIHR
DDQFKELEAN VRYLEDERRK VNNEKIEEEE KLKSEIEVLT LEKVEKGRCI ETLSRKVSEL
ESEISRLGSE IKARDDRTME MEKEVEKQRR ELEEVAEEKR EVIRQLCFSL DYSRDEYKRL
RIAFSGHPPT RPSSILAS
