NET5_MOUSE
ID NET5_MOUSE Reviewed; 452 AA.
AC Q3UQ22; B9EKG6;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2012, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Netrin-5;
DE AltName: Full=Netrin-1-like protein;
DE Flags: Precursor;
GN Name=Ntn5; Synonyms=Gm484;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=26858598; DOI=10.3389/fnmol.2016.00003;
RA Garrett A.M., Jucius T.J., Sigaud L.P., Tang F.L., Xiong W.C.,
RA Ackerman S.L., Burgess R.W.;
RT "Analysis of expression pattern and genetic deletion of netrin5 in the
RT developing mouse.";
RL Front. Mol. Neurosci. 9:3-3(2016).
CC -!- FUNCTION: Plays a role in neurogenesis. Prevents motor neuron cell body
CC migration out of the neural tube. {ECO:0000269|PubMed:26858598}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255}.
CC -!- DEVELOPMENTAL STAGE: Detected in boundary cap cells at 11.5 dpc,
CC expression is strongest between 15.5 and 17.5 dpc and is barely
CC detectable at birth. {ECO:0000269|PubMed:26858598}.
CC -!- DISRUPTION PHENOTYPE: Deficient mice exhibit ectopic motor neurons that
CC migrate out of the ventral horn and into the motor roots.
CC {ECO:0000269|PubMed:26858598}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE25222.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK142898; BAE25222.1; ALT_FRAME; mRNA.
DR EMBL; AC167242; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC150901; AAI50902.1; -; mRNA.
DR CCDS; CCDS39957.1; -.
DR RefSeq; NP_001028528.2; NM_001033356.3.
DR RefSeq; NP_001276622.1; NM_001289693.1.
DR AlphaFoldDB; Q3UQ22; -.
DR SMR; Q3UQ22; -.
DR BioGRID; 232594; 1.
DR STRING; 10090.ENSMUSP00000103371; -.
DR GlyGen; Q3UQ22; 1 site.
DR iPTMnet; Q3UQ22; -.
DR PhosphoSitePlus; Q3UQ22; -.
DR PaxDb; Q3UQ22; -.
DR PRIDE; Q3UQ22; -.
DR Antibodypedia; 31764; 52 antibodies from 14 providers.
DR Ensembl; ENSMUST00000107742; ENSMUSP00000103371; ENSMUSG00000070564.
DR GeneID; 243967; -.
DR KEGG; mmu:243967; -.
DR UCSC; uc009gwp.2; mouse.
DR CTD; 126147; -.
DR MGI; MGI:2685330; Ntn5.
DR VEuPathDB; HostDB:ENSMUSG00000070564; -.
DR eggNOG; KOG3512; Eukaryota.
DR GeneTree; ENSGT00940000161874; -.
DR HOGENOM; CLU_018213_0_0_1; -.
DR InParanoid; Q3UQ22; -.
DR OMA; SCPQACA; -.
DR OrthoDB; 858946at2759; -.
DR PhylomeDB; Q3UQ22; -.
DR TreeFam; TF352481; -.
DR BioGRID-ORCS; 243967; 2 hits in 71 CRISPR screens.
DR PRO; PR:Q3UQ22; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q3UQ22; protein.
DR Bgee; ENSMUSG00000070564; Expressed in gastrula and 35 other tissues.
DR ExpressionAtlas; Q3UQ22; baseline and differential.
DR Genevisible; Q3UQ22; MM.
DR GO; GO:0005604; C:basement membrane; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0009887; P:animal organ morphogenesis; IBA:GO_Central.
DR GO; GO:0016358; P:dendrite development; IBA:GO_Central.
DR GO; GO:0008045; P:motor neuron axon guidance; IBA:GO_Central.
DR GO; GO:0022008; P:neurogenesis; IMP:UniProtKB.
DR GO; GO:0009888; P:tissue development; IBA:GO_Central.
DR CDD; cd00055; EGF_Lam; 2.
DR Gene3D; 2.40.50.120; -; 1.
DR InterPro; IPR002049; LE_dom.
DR InterPro; IPR001134; Netrin_domain.
DR InterPro; IPR018933; Netrin_module_non-TIMP.
DR InterPro; IPR008993; TIMP-like_OB-fold.
DR Pfam; PF00053; Laminin_EGF; 2.
DR Pfam; PF01759; NTR; 1.
DR SMART; SM00643; C345C; 1.
DR SMART; SM00180; EGF_Lam; 2.
DR SUPFAM; SSF50242; SSF50242; 1.
DR PROSITE; PS00022; EGF_1; 2.
DR PROSITE; PS01248; EGF_LAM_1; 1.
DR PROSITE; PS50027; EGF_LAM_2; 2.
DR PROSITE; PS50189; NTR; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Disulfide bond; Glycoprotein;
KW Laminin EGF-like domain; Neurogenesis; Reference proteome; Repeat;
KW Secreted; Signal.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..452
FT /note="Netrin-5"
FT /id="PRO_0000320576"
FT DOMAIN 173..227
FT /note="Laminin EGF-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DOMAIN 228..277
FT /note="Laminin EGF-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00460"
FT DOMAIN 298..438
FT /note="NTR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00295"
FT CARBOHYD 303
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 173..182
FT /evidence="ECO:0000250"
FT DISULFID 175..191
FT /evidence="ECO:0000250"
FT DISULFID 193..202
FT /evidence="ECO:0000250"
FT DISULFID 205..225
FT /evidence="ECO:0000250"
FT DISULFID 228..240
FT /evidence="ECO:0000250"
FT DISULFID 230..247
FT /evidence="ECO:0000250"
FT DISULFID 249..258
FT /evidence="ECO:0000250"
FT DISULFID 261..275
FT /evidence="ECO:0000250"
FT DISULFID 298..376
FT /evidence="ECO:0000250"
FT DISULFID 302..378
FT /evidence="ECO:0000250"
FT DISULFID 317..438
FT /evidence="ECO:0000250"
SQ SEQUENCE 452 AA; 48837 MW; 66D3C10EDFF2C468 CRC64;
MTDYRTLFSS PGAGSTVTTP ITLSLLLLLS QATSDPCYDP GGRPRFCLPP VTQLVGKAAA
PCSQTCALPA ASPGPACNSS LTLDLDGSFL LTSVTLRFCT AGPPALVLSA AWATGGPWRP
LWRRPAWPGA LGGPKKVTFH SPPGPKTRIV ASYLRVEFGG KAGLVTTGVR GRCQCHGHAA
RCATRAQPPR CRCRHHTTGP GCESCRPSHR DWPWRPATPQ HPHPCLPCQC HPIGATGGMC
NQTSGQCSCK LGVTGLTCNR CGPGYQQSRS PRMPCQRIPE ATTTPATTPV ASRSDPQCQG
YCNVSVSSVH MSLQRYCQQD YVLHAQVSAS SSQPSEAVGP EWWRLAVHVL AVFKQRAWPV
RRGGQEAWVP RADLICGCLR LRPGADYLLL GRAAQTHDDD NYDPARLILN RHGLALPWRP
RWARPLRRLQ QKERGGACRG LLPPTRSPGP RN
