ID   NETNE_TRYCR             Reviewed;          13 AA.
AC   P84883;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   17-JUN-2020, entry version 23.
DE   RecName: Full=GPI-anchored glycoprotein NETNES;
DE   Flags: Fragment;
OS   Trypanosoma cruzi.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.
OX   NCBI_TaxID=5693;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-4; ASN-7; SER-9 AND SER-11,
RP   PHOSPHORYLATION AT SER-9 AND SER-11, STRUCTURE OF CARBOHYDRATE, GPI-ANCHOR
RP   AT ASP-13, AND MASS SPECTROMETRY.
RX   PubMed=15649890; DOI=10.1074/jbc.m412939200;
RA   Macrae J.I., Acosta-Serrano A., Morrice N.A., Mehlert A., Ferguson M.A.J.;
RT   "Structural characterization of NETNES, a novel glycoconjugate in
RT   Trypanosoma cruzi epimastigotes.";
RL   J. Biol. Chem. 280:12201-12211(2005).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC   -!- PTM: This peptide displays a high degree of glycan microheterogeneity
CC       resulting mainly from a variable number of mannose residues in each
CC       glycan. Both N-linked and O-linked glycans are high mannose.
CC       {ECO:0000269|PubMed:15649890}.
CC   -!- MASS SPECTROMETRY: Mass=5555; Method=Electrospray; Note=The measured
CC       mass is that of a major glycosylated and GPI-anchored form.;
CC       Evidence={ECO:0000269|PubMed:15649890};
CC   -!- MASS SPECTROMETRY: Mass=6770; Method=Electrospray; Note=The measured
CC       mass is that of a major glycosylated and GPI-anchored form.;
CC       Evidence={ECO:0000269|PubMed:15649890};
CC   -!- MASS SPECTROMETRY: Mass=719.8; Method=Electrospray; Note=The measured
CC       mass is that of the peptide after removal by PNGase F of all glycans
CC       and the GPI anchor.; Evidence={ECO:0000269|PubMed:15649890};
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DR   iPTMnet; P84883; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Cell membrane; Direct protein sequencing; Glycoprotein; GPI-anchor;
KW   Lipoprotein; Membrane; Phosphoprotein.
FT   PEPTIDE         1..13
FT                   /note="GPI-anchored glycoprotein NETNES"
FT                   /evidence="ECO:0000269|PubMed:15649890"
FT                   /id="PRO_0000248265"
FT   LIPID           13
FT                   /note="GPI-anchor amidated aspartate"
FT                   /evidence="ECO:0000269|PubMed:15649890"
FT   CARBOHYD        4
FT                   /note="N-linked (GlcNAc...) (high mannose) asparagine"
FT                   /evidence="ECO:0000269|PubMed:15649890"
FT   CARBOHYD        7
FT                   /note="N-linked (GlcNAc...) (high mannose) asparagine"
FT                   /evidence="ECO:0000269|PubMed:15649890"
FT   CARBOHYD        9
FT                   /note="O-linked (Man1P...) serine"
FT                   /evidence="ECO:0000269|PubMed:15649890"
FT   CARBOHYD        11
FT                   /note="O-linked (Man1P...) serine"
FT                   /evidence="ECO:0000269|PubMed:15649890"
FT   NON_TER         1
SQ   SEQUENCE   13 AA;  1393 MW;  8BEDE6ED60BE1451 CRC64;
     AQENETNESG SID