NEU1_HORSE
ID NEU1_HORSE Reviewed; 105 AA.
AC P01176; O77675;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 2.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Oxytocin-neurophysin 1;
DE Short=OT-NPI;
DE Contains:
DE RecName: Full=Oxytocin;
DE AltName: Full=Ocytocin;
DE Contains:
DE RecName: Full=Neurophysin 1;
DE Flags: Precursor; Fragment;
GN Name=OXT;
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796;
RN [1]
RP PROTEIN SEQUENCE OF 1-9, AND AMIDATION AT GLY-9.
RX PubMed=13629241;
RA Acher R., Chauvet J., Lenci M.T.;
RT "Oxytocin and vasopressin from horses.";
RL Bull. Soc. Chim. Biol. 40:2005-2018(1958).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-69.
RX PubMed=10343920; DOI=10.1016/s0739-7240(99)00008-9;
RA Behrendt-Adam C.Y., Adams M.H., Simpson K.S., McDowell K.J.;
RT "Oxytocin-neurophysin I mRNA abundance in equine uterine endometrium.";
RL Domest. Anim. Endocrinol. 16:183-192(1999).
RN [3]
RP PROTEIN SEQUENCE OF 13-105.
RX PubMed=7271775; DOI=10.1016/s0006-291x(81)80218-5;
RA Chauvet M.-T., Chauvet J., Acher R.;
RT "Identification of neurophysins: complete aminoacid sequence of horse VLDV-
RT neurophysin.";
RL Biochem. Biophys. Res. Commun. 100:600-605(1981).
CC -!- FUNCTION: Neurophysin 1 specifically binds oxytocin.
CC -!- FUNCTION: Oxytocin causes contraction of the smooth muscle of the
CC uterus and of the mammary gland. Acts by binding to oxytocin receptor
CC (OXTR) (By similarity). {ECO:0000250|UniProtKB:P01178}.
CC -!- SUBUNIT: Interacts with oxytocin receptor (Ki=1.5 nM) (By similarity).
CC Interacts with vasopressin V1aR/AVPR1A (Ki=37 nM), V1bR/AVPR1B (Ki=222
CC nM), and V2R/AVPR2 receptors (Ki=823 nM) (By similarity).
CC {ECO:0000250|UniProtKB:P01178}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- MISCELLANEOUS: 'GKR' has been placed at positions 10-12 by homology
CC with the complete sequence of the other precursors.
CC -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR EMBL; AF076673; AAC27491.1; -; mRNA.
DR PIR; A90735; NFHO1.
DR AlphaFoldDB; P01176; -.
DR SMR; P01176; -.
DR InParanoid; P01176; -.
DR Proteomes; UP000002281; Unplaced.
DR GO; GO:0005615; C:extracellular space; ISS:AgBase.
DR GO; GO:0030141; C:secretory granule; IBA:GO_Central.
DR GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR GO; GO:0005184; F:neuropeptide hormone activity; IBA:GO_Central.
DR GO; GO:0031855; F:oxytocin receptor binding; IBA:GO_Central.
DR GO; GO:0043207; P:response to external biotic stimulus; ISS:AgBase.
DR GO; GO:0032094; P:response to food; ISS:AgBase.
DR GO; GO:0009612; P:response to mechanical stimulus; ISS:AgBase.
DR Gene3D; 2.60.9.10; -; 1.
DR InterPro; IPR000981; Neurhyp_horm.
DR InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR PANTHER; PTHR11681; PTHR11681; 1.
DR Pfam; PF00220; Hormone_4; 1.
DR Pfam; PF00184; Hormone_5; 1.
DR PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR PRINTS; PR00831; NEUROPHYSIN.
DR SMART; SM00003; NH; 1.
DR SUPFAM; SSF49606; SSF49606; 1.
DR PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Hormone; Reference proteome; Secreted.
FT PEPTIDE 1..9
FT /note="Oxytocin"
FT /id="PRO_0000020493"
FT CHAIN 13..105
FT /note="Neurophysin 1"
FT /id="PRO_0000020494"
FT MOD_RES 9
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:13629241"
FT DISULFID 1..6
FT DISULFID 22..66
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 25..39
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 33..56
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 40..46
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 73..85
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 79..97
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 86..91
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT CONFLICT 26
FT /note="G -> A (in Ref. 2; AAC27491)"
FT /evidence="ECO:0000305"
FT NON_TER 1
SQ SEQUENCE 105 AA; 10723 MW; 4C0C52ED51C7521D CRC64;
CYIQNCPLGG KRAALDLDVR KCLPCGPGGK GRCFGPSICC GDELGCFVGT AEALRCQEEN
YLPSPCQSGQ KPCGSGGRCA AAGICCSPDG CLADPSCHDE AAFSQ
