NEU1_RAT
ID NEU1_RAT Reviewed; 125 AA.
AC P01179; Q53WU2;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Oxytocin-neurophysin 1;
DE Short=OT-NPI;
DE Contains:
DE RecName: Full=Oxytocin;
DE AltName: Full=Ocytocin;
DE Contains:
DE RecName: Full=Neurophysin 1;
DE Flags: Precursor;
GN Name=Oxt; Synonyms=Ot;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6326097; DOI=10.1073/pnas.81.7.2006;
RA Ivell R., Richter D.;
RT "Structure and comparison of the oxytocin and vasopressin genes from rat.";
RL Proc. Natl. Acad. Sci. U.S.A. 81:2006-2010(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3768139; DOI=10.1515/bchm3.1986.367.2.695;
RA Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H.,
RA Richter D.;
RT "The neurohypophyseal hormones vasopressin and oxytocin. Precursor
RT structure, synthesis and regulation.";
RL Biol. Chem. Hoppe-Seyler 367:695-704(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3203740; DOI=10.1016/0014-5793(88)81003-2;
RA Mohr E., Bahnsen U., Kiessling C., Richter D.;
RT "Expression of the vasopressin and oxytocin genes in rats occurs in
RT mutually exclusive sets of hypothalamic neurons.";
RL FEBS Lett. 242:144-148(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Wistar; TISSUE=Liver;
RX PubMed=1706187; DOI=10.1089/dna.1991.10.81;
RA Schmitz E., Mohr E., Richter D.;
RT "Rat vasopressin and oxytocin genes are linked by a long interspersed
RT repeated DNA element (LINE): sequence and transcriptional analysis of
RT LINE.";
RL DNA Cell Biol. 10:81-91(1991).
RN [5]
RP PROTEIN SEQUENCE OF 32-125.
RX PubMed=7036996; DOI=10.1016/0006-291x(81)90493-9;
RA Chauvet M.-T., Chauvet J., Acher R.;
RT "Identification of rat neurophysins: complete amino acid sequences of
RT MSEL- and VLDV-neurophysins.";
RL Biochem. Biophys. Res. Commun. 103:595-603(1981).
RN [6]
RP PROTEIN SEQUENCE OF 32-100.
RX PubMed=7238864; DOI=10.1016/0014-5793(81)81028-9;
RA North W.G., Mitchell T.I.;
RT "Evolution of neurophysin proteins: partial amino acid sequences of rat
RT neurophysins.";
RL FEBS Lett. 126:41-44(1981).
RN [7]
RP PROTEIN SEQUENCE OF 32-64.
RX PubMed=891987; DOI=10.1016/0014-5793(77)80478-x;
RA Schlesinger D.H., Pickering B.T., Watkins W.B., Peek J.C., Moore L.G.,
RA Audhya T.K., Walter R.;
RT "A comparative study of partial neurophysin protein sequences of cod,
RT guinea pig, rat and sheep.";
RL FEBS Lett. 80:371-373(1977).
RN [8]
RP PROTEIN SEQUENCE OF 20-28, AND AMIDATION AT GLY-28.
RX PubMed=5150741; DOI=10.1016/s0300-9084(71)80199-2;
RA Chauvet J., Chauvet M.-T., Acher R.;
RT "Evolution of neurohypophyseal hormones: isolation of active principles
RT from rabbits and rats.";
RL Biochimie 53:1099-1104(1971).
CC -!- FUNCTION: Neurophysin 1 specifically binds oxytocin.
CC -!- FUNCTION: Oxytocin causes contraction of the smooth muscle of the
CC uterus and of the mammary gland. Acts by binding to oxytocin receptor
CC (OXTR) (By similarity). {ECO:0000250|UniProtKB:P01178}.
CC -!- SUBUNIT: Interacts with oxytocin receptor (Ki=1.5 nM) (By similarity).
CC Interacts with vasopressin V1aR/AVPR1A (Ki=37 nM), V1bR/AVPR1B (Ki=222
CC nM), and V2R/AVPR2 receptors (Ki=823 nM) (By similarity).
CC {ECO:0000250|UniProtKB:P01178}.
CC -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR EMBL; K01701; AAA98733.1; -; Genomic_DNA.
DR EMBL; M25649; AAA41773.1; -; mRNA.
DR EMBL; X12792; CAA31281.1; -; Genomic_DNA.
DR EMBL; X59496; CAA42085.1; -; Genomic_DNA.
DR PIR; A21183; NFRT1.
DR RefSeq; NP_037128.1; NM_012996.3.
DR AlphaFoldDB; P01179; -.
DR SMR; P01179; -.
DR STRING; 10116.ENSRNOP00000028829; -.
DR PaxDb; P01179; -.
DR DNASU; 25504; -.
DR Ensembl; ENSRNOT00000028829; ENSRNOP00000028829; ENSRNOG00000021225.
DR GeneID; 25504; -.
DR KEGG; rno:25504; -.
DR UCSC; RGD:3238; rat.
DR CTD; 5020; -.
DR RGD; 3238; Oxt.
DR eggNOG; ENOG502S2CT; Eukaryota.
DR GeneTree; ENSGT00390000004511; -.
DR HOGENOM; CLU_125770_1_0_1; -.
DR InParanoid; P01179; -.
DR OMA; YIQNCPP; -.
DR OrthoDB; 1548839at2759; -.
DR PhylomeDB; P01179; -.
DR TreeFam; TF333018; -.
DR Reactome; R-RNO-388479; Vasopressin-like receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:P01179; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Bgee; ENSRNOG00000021225; Expressed in ovary and 1 other tissue.
DR Genevisible; P01179; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0005576; C:extracellular region; ISO:RGD.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0030141; C:secretory granule; IDA:RGD.
DR GO; GO:0043195; C:terminal bouton; IDA:RGD.
DR GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR GO; GO:0005184; F:neuropeptide hormone activity; IDA:RGD.
DR GO; GO:0031855; F:oxytocin receptor binding; IDA:RGD.
DR GO; GO:0042756; P:drinking behavior; IDA:RGD.
DR GO; GO:0042755; P:eating behavior; IDA:RGD.
DR GO; GO:0007565; P:female pregnancy; IEP:RGD.
DR GO; GO:0007625; P:grooming behavior; IDA:RGD.
DR GO; GO:0007507; P:heart development; IEP:RGD.
DR GO; GO:0042538; P:hyperosmotic salinity response; IEP:RGD.
DR GO; GO:0007595; P:lactation; TAS:RGD.
DR GO; GO:0060179; P:male mating behavior; IEP:RGD.
DR GO; GO:0002125; P:maternal aggressive behavior; IEP:RGD.
DR GO; GO:0042711; P:maternal behavior; IDA:RGD.
DR GO; GO:0007613; P:memory; IEP:RGD.
DR GO; GO:0045776; P:negative regulation of blood pressure; IMP:RGD.
DR GO; GO:0060455; P:negative regulation of gastric acid secretion; IDA:RGD.
DR GO; GO:0035811; P:negative regulation of urine volume; IDA:RGD.
DR GO; GO:0007567; P:parturition; TAS:RGD.
DR GO; GO:0045777; P:positive regulation of blood pressure; IDA:RGD.
DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; ISS:YuBioLab.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD.
DR GO; GO:0045925; P:positive regulation of female receptivity; IDA:RGD.
DR GO; GO:0060450; P:positive regulation of hindgut contraction; IDA:RGD.
DR GO; GO:0010701; P:positive regulation of norepinephrine secretion; IDA:RGD.
DR GO; GO:0045778; P:positive regulation of ossification; IDA:RGD.
DR GO; GO:0060406; P:positive regulation of penile erection; IDA:RGD.
DR GO; GO:0032308; P:positive regulation of prostaglandin secretion; IDA:RGD.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; IDA:RGD.
DR GO; GO:0051965; P:positive regulation of synapse assembly; IDA:RGD.
DR GO; GO:0050806; P:positive regulation of synaptic transmission; IDA:RGD.
DR GO; GO:0070474; P:positive regulation of uterine smooth muscle contraction; IDA:RGD.
DR GO; GO:0044058; P:regulation of digestive system process; IDA:RGD.
DR GO; GO:0002027; P:regulation of heart rate; IDA:RGD.
DR GO; GO:0051930; P:regulation of sensory perception of pain; IDA:RGD.
DR GO; GO:0014823; P:response to activity; IEP:RGD.
DR GO; GO:0001975; P:response to amphetamine; IEP:RGD.
DR GO; GO:0051591; P:response to cAMP; IEP:RGD.
DR GO; GO:0042220; P:response to cocaine; IEP:RGD.
DR GO; GO:0051602; P:response to electrical stimulus; IEP:RGD.
DR GO; GO:0032355; P:response to estradiol; IEP:RGD.
DR GO; GO:0045472; P:response to ether; IEP:RGD.
DR GO; GO:0043207; P:response to external biotic stimulus; ISS:AgBase.
DR GO; GO:0032094; P:response to food; IEP:RGD.
DR GO; GO:0051384; P:response to glucocorticoid; IEP:RGD.
DR GO; GO:0009612; P:response to mechanical stimulus; ISS:AgBase.
DR GO; GO:0014070; P:response to organic cyclic compound; IDA:RGD.
DR GO; GO:0043434; P:response to peptide hormone; IEP:RGD.
DR GO; GO:0032570; P:response to progesterone; IDA:RGD.
DR GO; GO:0034695; P:response to prostaglandin E; IEP:RGD.
DR GO; GO:0032526; P:response to retinoic acid; IEP:RGD.
DR GO; GO:0048545; P:response to steroid hormone; IEP:RGD.
DR GO; GO:0009744; P:response to sucrose; IEP:RGD.
DR GO; GO:0030431; P:sleep; IDA:RGD.
DR GO; GO:0035176; P:social behavior; IDA:RGD.
DR GO; GO:0042713; P:sperm ejaculation; IDA:RGD.
DR Gene3D; 2.60.9.10; -; 1.
DR InterPro; IPR000981; Neurhyp_horm.
DR InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR PANTHER; PTHR11681; PTHR11681; 1.
DR Pfam; PF00220; Hormone_4; 1.
DR Pfam; PF00184; Hormone_5; 1.
DR PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR PRINTS; PR00831; NEUROPHYSIN.
DR SMART; SM00003; NH; 1.
DR SUPFAM; SSF49606; SSF49606; 1.
DR PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Hormone; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:5150741"
FT PEPTIDE 20..28
FT /note="Oxytocin"
FT /evidence="ECO:0000269|PubMed:5150741"
FT /id="PRO_0000020503"
FT CHAIN 32..125
FT /note="Neurophysin 1"
FT /id="PRO_0000020504"
FT MOD_RES 28
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:5150741"
FT DISULFID 20..25
FT /evidence="ECO:0000269|PubMed:5150741"
FT DISULFID 41..85
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 44..58
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 52..75
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 59..65
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 92..104
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 98..116
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 105..110
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT CONFLICT 100
FT /note="T -> A (in Ref. 6; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 124..125
FT /note="ER -> Q (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 125 AA; 12823 MW; DC98F90BADC5551F CRC64;
MACPSLACCL LGLLALTSAC YIQNCPLGGK RAALDLDMRK CLPCGPGGKG RCFGPSICCA
DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAT AGICCSPDGC RTDPACDPES
AFSER
