ID   NEU2_CAVPO              Reviewed;         144 AA.
AC   P10769;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Vasopressin-neurophysin 2-copeptin;
DE   AltName: Full=AVP-NPII;
DE   Contains:
DE     RecName: Full=Arg-vasopressin;
DE     AltName: Full=Arginine-vasopressin;
DE   Contains:
DE     RecName: Full=Neurophysin 2;
DE     AltName: Full=Neurophysin-II;
DE   Contains:
DE     RecName: Full=Copeptin;
DE   Flags: Precursor;
GN   Name=AVP;
OS   Cavia porcellus (Guinea pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC   Cavia.
OX   NCBI_TaxID=10141;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=3803579; DOI=10.1016/0014-5793(87)81294-2;
RA   Chauvet M.-T., Rouille Y., Chauvet J., Acher R.;
RT   "Guinea pig neurohypophysial hormones. Peculiar processing of the three-
RT   domain vasopressin precursor.";
RL   FEBS Lett. 210:40-44(1987).
RN   [2]
RP   PROTEIN SEQUENCE OF 13-144.
RX   PubMed=3595848; DOI=10.1016/0014-5793(87)80659-2;
RA   Chauvet J., Chauvet M.-T., Acher R.;
RT   "Conformation limited proteolysis in the common neurophysin-copeptin
RT   precursor shown by trypsin-sepharose chromatographic proteolysis.";
RL   FEBS Lett. 217:180-183(1987).
RN   [3]
RP   PROTEIN SEQUENCE OF 13-105.
RX   PubMed=3436704; DOI=10.1111/j.1399-3011.1987.tb03379.x;
RA   Chauvet M.-T., Chauvet J., Acher R.;
RT   "Guinea pig MSEL-neurophysin. Sequence comparison of eight mammalian MSEL-
RT   neurophysins.";
RL   Int. J. Pept. Protein Res. 30:676-682(1987).
RN   [4]
RP   PROTEIN SEQUENCE OF 95-132, AND AMIDATION AT GLY-9.
RX   PubMed=3081370; DOI=10.1016/0014-5793(86)80320-9;
RA   Chauvet M.-T., Chauvet J., Acher R.;
RT   "Guinea pig copeptin. The glycopeptide domain of the vasopressin
RT   precursor.";
RL   FEBS Lett. 197:169-172(1986).
CC   -!- FUNCTION: Neurophysin 2 specifically binds vasopressin.
CC   -!- FUNCTION: Vasopressin has a direct antidiuretic action on the kidney,
CC       it also causes vasoconstriction of the peripheral vessels. Acts by
CC       binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and
CC       V2R/AVPR2) (By similarity). {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBUNIT: Interacts with vasopressin receptors V1bR/AVPR1B (Ki=85 pM),
CC       V1aR/AVPR1A (Ki=0.6 nM) and V2R/AVPR2 (Ki=4.9 nM) (By similarity).
CC       Interacts with oxytocin receptor (OXTR) (Ki=110 nM) (By similarity).
CC       {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A29101; A29101.
DR   AlphaFoldDB; P10769; -.
DR   SMR; P10769; -.
DR   STRING; 10141.ENSCPOP00000015497; -.
DR   eggNOG; ENOG502S21K; Eukaryota.
DR   HOGENOM; CLU_125770_0_0_1; -.
DR   InParanoid; P10769; -.
DR   Proteomes; UP000005447; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   GO; GO:0042310; P:vasoconstriction; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.9.10; -; 1.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR   InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR   PANTHER; PTHR11681; PTHR11681; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   SMART; SM00003; NH; 1.
DR   SUPFAM; SSF49606; SSF49606; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Vasoactive; Vasoconstrictor.
FT   PEPTIDE         1..9
FT                   /note="Arg-vasopressin"
FT                   /id="PRO_0000020512"
FT   CHAIN           13..105
FT                   /note="Neurophysin 2"
FT                   /id="PRO_0000020513"
FT   PEPTIDE         107..144
FT                   /note="Copeptin"
FT                   /id="PRO_0000020514"
FT   SITE            9
FT                   /note="Important for agonist activity on V1aR/AVPR1A"
FT                   /evidence="ECO:0000250|UniProtKB:P01185"
FT   MOD_RES         9
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000269|PubMed:3081370"
FT   CARBOHYD        112
FT                   /note="N-linked (GlcNAc...) asparagine"
FT   DISULFID        1..6
FT   DISULFID        22..66
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        25..39
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        33..56
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        40..46
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        73..85
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        79..97
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        86..91
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
SQ   SEQUENCE   144 AA;  15068 MW;  CE2B18A162C9ABEA CRC64;
     CYFQNCPRGG KRALSDTELR QCLPCGPGGQ GRCFGPSICC ADALGCFVGT AEALRCQEEN
     YLPSPCQSGQ KPCGSGGRCA ANGVCCNDES CVIEPECREE FHRPVRAGDR SNVTQLDGPA
     GALLLRLMQL AGAPEPQPAA PGGY