NEU2_MOUSE
ID NEU2_MOUSE Reviewed; 168 AA.
AC P35455;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Vasopressin-neurophysin 2-copeptin;
DE AltName: Full=AVP-NPII;
DE Contains:
DE RecName: Full=Arg-vasopressin;
DE AltName: Full=Arginine-vasopressin;
DE Contains:
DE RecName: Full=Neurophysin 2;
DE AltName: Full=Neurophysin-I;
DE Contains:
DE RecName: Full=Copeptin;
DE Flags: Precursor;
GN Name=Avp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=2176709; DOI=10.1016/0169-328x(90)90045-f;
RA Hara Y., Battey J., Gainer H.;
RT "Structure of mouse vasopressin and oxytocin genes.";
RL Brain Res. Mol. Brain Res. 8:319-324(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Neurophysin 2 specifically binds vasopressin.
CC -!- FUNCTION: Vasopressin has a direct antidiuretic action on the kidney,
CC it also causes vasoconstriction of the peripheral vessels. Acts by
CC binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and
CC V2R/AVPR2) (By similarity). {ECO:0000250|UniProtKB:P01185}.
CC -!- SUBUNIT: Interacts with vasopressin receptors V1bR/AVPR1B (Ki=85 pM),
CC V1aR/AVPR1A (Ki=0.6 nM) and V2R/AVPR2 (Ki=4.9 nM) (By similarity).
CC Interacts with oxytocin receptor (OXTR) (Ki=110 nM) (By similarity).
CC {ECO:0000250|UniProtKB:P01185}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR EMBL; M88354; AAC42027.1; -; Genomic_DNA.
DR EMBL; BC051997; AAH51997.1; -; mRNA.
DR CCDS; CCDS16746.1; -.
DR PIR; B43755; B43755.
DR RefSeq; NP_033862.1; NM_009732.2.
DR AlphaFoldDB; P35455; -.
DR SMR; P35455; -.
DR STRING; 10090.ENSMUSP00000035551; -.
DR GlyConnect; 2816; 3 N-Linked glycans (1 site).
DR GlyGen; P35455; 1 site, 3 N-linked glycans (1 site).
DR PhosphoSitePlus; P35455; -.
DR CPTAC; non-CPTAC-3931; -.
DR PaxDb; P35455; -.
DR PeptideAtlas; P35455; -.
DR PRIDE; P35455; -.
DR ProteomicsDB; 287383; -.
DR Antibodypedia; 7264; 320 antibodies from 31 providers.
DR Ensembl; ENSMUST00000046001; ENSMUSP00000035551; ENSMUSG00000037727.
DR GeneID; 11998; -.
DR KEGG; mmu:11998; -.
DR UCSC; uc008mjh.1; mouse.
DR CTD; 551; -.
DR MGI; MGI:88121; Avp.
DR VEuPathDB; HostDB:ENSMUSG00000037727; -.
DR eggNOG; ENOG502S21K; Eukaryota.
DR GeneTree; ENSGT00390000004511; -.
DR HOGENOM; CLU_125770_0_0_1; -.
DR InParanoid; P35455; -.
DR OMA; GCVVDSD; -.
DR OrthoDB; 1548839at2759; -.
DR PhylomeDB; P35455; -.
DR TreeFam; TF333018; -.
DR Reactome; R-MMU-388479; Vasopressin-like receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR Reactome; R-MMU-432040; Vasopressin regulates renal water homeostasis via Aquaporins.
DR Reactome; R-MMU-879518; Transport of organic anions.
DR Reactome; R-MMU-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-MMU-8856828; Clathrin-mediated endocytosis.
DR BioGRID-ORCS; 11998; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Avp; mouse.
DR PRO; PR:P35455; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P35455; protein.
DR Bgee; ENSMUSG00000037727; Expressed in paraventricular nucleus of hypothalamus and 58 other tissues.
DR ExpressionAtlas; P35455; baseline and differential.
DR Genevisible; P35455; MM.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; ISO:MGI.
DR GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR GO; GO:0005184; F:neuropeptide hormone activity; ISO:MGI.
DR GO; GO:0004672; F:protein kinase activity; ISO:MGI.
DR GO; GO:0031894; F:V1A vasopressin receptor binding; ISO:MGI.
DR GO; GO:0031895; F:V1B vasopressin receptor binding; ISO:MGI.
DR GO; GO:0070371; P:ERK1 and ERK2 cascade; ISO:MGI.
DR GO; GO:0007625; P:grooming behavior; ISO:MGI.
DR GO; GO:0042538; P:hyperosmotic salinity response; IEA:Ensembl.
DR GO; GO:0007626; P:locomotory behavior; ISO:MGI.
DR GO; GO:0002125; P:maternal aggressive behavior; ISO:MGI.
DR GO; GO:0042711; P:maternal behavior; ISO:MGI.
DR GO; GO:0050891; P:multicellular organismal water homeostasis; ISO:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:MGI.
DR GO; GO:0007621; P:negative regulation of female receptivity; ISO:MGI.
DR GO; GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; ISO:MGI.
DR GO; GO:0051970; P:negative regulation of transmission of nerve impulse; ISO:MGI.
DR GO; GO:0043084; P:penile erection; ISO:MGI.
DR GO; GO:0030307; P:positive regulation of cell growth; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0032849; P:positive regulation of cellular pH reduction; ISO:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0014049; P:positive regulation of glutamate secretion; ISO:MGI.
DR GO; GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; ISO:MGI.
DR GO; GO:0031394; P:positive regulation of prostaglandin biosynthetic process; ISO:MGI.
DR GO; GO:0003084; P:positive regulation of systemic arterial blood pressure; ISO:MGI.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; ISO:MGI.
DR GO; GO:0070528; P:protein kinase C signaling; ISO:MGI.
DR GO; GO:0035813; P:regulation of renal sodium excretion; ISO:MGI.
DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl.
DR GO; GO:0035094; P:response to nicotine; IEA:Ensembl.
DR GO; GO:0033574; P:response to testosterone; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; ISO:MGI.
DR GO; GO:0035176; P:social behavior; ISO:MGI.
DR GO; GO:0042310; P:vasoconstriction; IEA:UniProtKB-KW.
DR GO; GO:0046718; P:viral entry into host cell; ISO:MGI.
DR Gene3D; 2.60.9.10; -; 1.
DR InterPro; IPR000981; Neurhyp_horm.
DR InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR PANTHER; PTHR11681; PTHR11681; 1.
DR Pfam; PF00220; Hormone_4; 1.
DR Pfam; PF00184; Hormone_5; 1.
DR PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR PRINTS; PR00831; NEUROPHYSIN.
DR SMART; SM00003; NH; 1.
DR SUPFAM; SSF49606; SSF49606; 1.
DR PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE 2: Evidence at transcript level;
KW Amidation; Cleavage on pair of basic residues; Disulfide bond;
KW Glycoprotein; Hormone; Reference proteome; Secreted; Signal; Vasoactive;
KW Vasoconstrictor.
FT SIGNAL 1..23
FT /evidence="ECO:0000250"
FT PEPTIDE 24..32
FT /note="Arg-vasopressin"
FT /id="PRO_0000020518"
FT CHAIN 36..128
FT /note="Neurophysin 2"
FT /id="PRO_0000020519"
FT PEPTIDE 130..168
FT /note="Copeptin"
FT /id="PRO_0000020520"
FT SITE 32
FT /note="Important for agonist activity on V1aR/AVPR1A"
FT /evidence="ECO:0000250|UniProtKB:P01185"
FT MOD_RES 32
FT /note="Glycine amide"
FT /evidence="ECO:0000250|UniProtKB:P01185"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 24..29
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 45..89
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 48..62
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 56..79
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 63..69
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 96..108
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 102..120
FT /evidence="ECO:0000250|UniProtKB:P01175"
FT DISULFID 109..114
FT /evidence="ECO:0000250|UniProtKB:P01175"
SQ SEQUENCE 168 AA; 17900 MW; 234F6D826E0BBEE9 CRC64;
MLARMLNTTL SACFLSLLAF SSACYFQNCP RGGKRAISDM ELRQCLPCGP GGKGRCFGPS
ICCADELGCF VGTAEALRCQ EENYLPSPCQ SGQKPCGSGG RCAAVGICCS DESCVAEPEC
HDGFFRLTRA REPSNATQLD GPARALLLRL VQLAGTRESV DSAKPRVY
