ID   NEU2_PIG                Reviewed;         166 AA.
AC   P01183;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 2.
DT   25-MAY-2022, entry version 127.
DE   RecName: Full=Vasopressin-neurophysin 2-copeptin;
DE   AltName: Full=AVP-NPII;
DE   Contains:
DE     RecName: Full=Lys-vasopressin;
DE   Contains:
DE     RecName: Full=Neurophysin 2;
DE     AltName: Full=Neurophysin-I/-III;
DE   Contains:
DE     RecName: Full=Copeptin;
DE   Flags: Precursor;
GN   Name=AVP;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=3768139; DOI=10.1515/bchm3.1986.367.2.695;
RA   Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H.,
RA   Richter D.;
RT   "The neurohypophyseal hormones vasopressin and oxytocin. Precursor
RT   structure, synthesis and regulation.";
RL   Biol. Chem. Hoppe-Seyler 367:695-704(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1971513; DOI=10.1677/jme.0.0040151;
RA   Rehbein M., Richter D.;
RT   "Poly(A) tail length of oxytocin- and lysine vasopressin-encoding mRNAs
RT   increases during development in the porcine hypothalamus.";
RL   J. Mol. Endocrinol. 4:151-158(1990).
RN   [3]
RP   PROTEIN SEQUENCE OF 20-28.
RA   Popenoe E.A., Lawler H.C., du Vigneaud V.;
RT   "Partial purification and amino acid content of vasopressin from hog
RT   posterior pituitary glands.";
RL   J. Am. Chem. Soc. 74:3713-3713(1952).
RN   [4]
RP   PROTEIN SEQUENCE OF 32-123.
RX   PubMed=4940405; DOI=10.1016/s0021-9258(18)61832-0;
RA   Wuu T.-C., Crumm S.E., Saffran M.;
RT   "Amino acid sequence of porcine neurophysin-I.";
RL   J. Biol. Chem. 246:6043-6063(1971).
RN   [5]
RP   PROTEIN SEQUENCE OF 118-126.
RX   PubMed=944186; DOI=10.1016/s0021-9258(17)33549-4;
RA   Wuu T.-C., Crumm S.E.;
RT   "Characterization of porcine neurophysin. III. Its resemblance and possible
RT   relationship to porcine neurophysin I.";
RL   J. Biol. Chem. 251:2735-2739(1976).
RN   [6]
RP   PROTEIN SEQUENCE OF 32-126.
RX   PubMed=1001445; DOI=10.1016/0014-5793(76)80953-2;
RA   Chauvet M.-T., Codogno P., Chauvet J., Acher R.;
RT   "Characterization of porcine MSEL-neurophysin.";
RL   FEBS Lett. 71:291-293(1976).
RN   [7]
RP   PROTEIN SEQUENCE OF 128-166.
RX   PubMed=465021; DOI=10.1016/s0006-291x(79)80007-8;
RA   Smyth D.G., Massey D.E.;
RT   "A new glycopeptide in pig, ox and sheep pituitary.";
RL   Biochem. Biophys. Res. Commun. 87:1006-1010(1979).
RN   [8]
RP   PROTEIN SEQUENCE OF 128-144.
RX   PubMed=4673067; DOI=10.1111/j.1432-1033.1972.tb01919.x;
RA   Holwerda D.A.;
RT   "A glycopeptide from the posterior lobe of pig pituitaries. 2. Primary
RT   structure.";
RL   Eur. J. Biochem. 28:340-346(1972).
CC   -!- FUNCTION: Neurophysin 2 specifically binds vasopressin.
CC   -!- FUNCTION: Vasopressin has a direct antidiuretic action on the kidney,
CC       it also causes vasoconstriction of the peripheral vessels. Acts by
CC       binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and
CC       V2R/AVPR2) (By similarity). {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBUNIT: Interacts with vasopressin receptors V1bR/AVPR1B (Ki=85 pM),
CC       V1aR/AVPR1A (Ki=0.6 nM) and V2R/AVPR2 (Ki=4.9 nM) (By similarity).
CC       Interacts with oxytocin receptor (OXTR) (Ki=110 nM) (By similarity).
CC       {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: A shorter neurophysin molecule (32-123) is called neurophysin-I
CC       and is derived from the complete protein (called neurophysin III) by
CC       proteolytic degradation (in vivo or after extraction).
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M25644; AAA31143.1; -; mRNA.
DR   PIR; A60970; NVPG2.
DR   RefSeq; NP_999117.1; NM_213952.2.
DR   AlphaFoldDB; P01183; -.
DR   SMR; P01183; -.
DR   PaxDb; P01183; -.
DR   PRIDE; P01183; -.
DR   Ensembl; ENSSSCT00025060715; ENSSSCP00025025756; ENSSSCG00025044667.
DR   Ensembl; ENSSSCT00030049131; ENSSSCP00030022239; ENSSSCG00030035426.
DR   Ensembl; ENSSSCT00035056711; ENSSSCP00035022809; ENSSSCG00035042676.
DR   Ensembl; ENSSSCT00040038404; ENSSSCP00040016042; ENSSSCG00040028620.
DR   Ensembl; ENSSSCT00045003768; ENSSSCP00045002384; ENSSSCG00045002408.
DR   Ensembl; ENSSSCT00050079715; ENSSSCP00050034263; ENSSSCG00050058482.
DR   Ensembl; ENSSSCT00060014591; ENSSSCP00060005660; ENSSSCG00060011180.
DR   Ensembl; ENSSSCT00065087661; ENSSSCP00065038353; ENSSSCG00065063862.
DR   GeneID; 396995; -.
DR   KEGG; ssc:396995; -.
DR   CTD; 551; -.
DR   eggNOG; ENOG502S21K; Eukaryota.
DR   InParanoid; P01183; -.
DR   OrthoDB; 1548839at2759; -.
DR   Reactome; R-SSC-388479; Vasopressin-like receptors.
DR   Reactome; R-SSC-416476; G alpha (q) signalling events.
DR   Reactome; R-SSC-879518; Transport of organic anions.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   GO; GO:0042310; P:vasoconstriction; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.9.10; -; 1.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR   InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR   PANTHER; PTHR11681; PTHR11681; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   SMART; SM00003; NH; 1.
DR   SUPFAM; SSF49606; SSF49606; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Signal; Vasoactive; Vasoconstrictor.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|Ref.3"
FT   PEPTIDE         20..28
FT                   /note="Lys-vasopressin"
FT                   /id="PRO_0000020521"
FT   CHAIN           32..126
FT                   /note="Neurophysin 2"
FT                   /id="PRO_0000020522"
FT   PEPTIDE         128..166
FT                   /note="Copeptin"
FT                   /id="PRO_0000020523"
FT   SITE            28
FT                   /note="Important for agonist activity on V1aR/AVPR1A"
FT                   /evidence="ECO:0000250|UniProtKB:P01185"
FT   MOD_RES         28
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000250|UniProtKB:P01185"
FT   CARBOHYD        133
FT                   /note="N-linked (GlcNAc...) asparagine"
FT   DISULFID        20..25
FT   DISULFID        41..85
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        44..58
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        52..75
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        59..65
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        92..104
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        98..116
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        105..110
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
SQ   SEQUENCE   166 AA;  17196 MW;  F2AED365892B1CD2 CRC64;
     MPDATLPACF LGLLALTSAC YFQNCPKGGK RAMSDLELRQ CLPCGPGGKG RCFGPSICCG
     DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAA AGICCNDESC VTEPECREGA
     SFLRRARASD RSNATLLDGP SGALLLRLVQ LAGAPEPAEP AQPGVY