ID   NEU2_SHEEP              Reviewed;         147 AA.
AC   P01181;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   07-OCT-2020, entry version 100.
DE   RecName: Full=Vasopressin-neurophysin 2-copeptin;
DE   AltName: Full=AVP-NPII;
DE   Contains:
DE     RecName: Full=Arg-vasopressin;
DE     AltName: Full=Arginine-vasopressin;
DE   Contains:
DE     RecName: Full=Neurophysin 2;
DE     AltName: Full=Neurophysin-III;
DE   Contains:
DE     RecName: Full=Copeptin;
DE   Flags: Precursor; Fragment;
GN   Name=AVP;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Caprinae; Ovis.
OX   NCBI_TaxID=9940;
RN   [1]
RP   PROTEIN SEQUENCE OF 1-9, AND AMIDATION AT GLY-9.
RA   Acher R., Chauvet J., Lenci M.T.;
RT   "Purification and structure of sheep oxytocin and vasopressin.";
RL   C. R. Hebd. Seances Acad. Sci., D, Sci. Nat. 248:1435-1438(1959).
RN   [2]
RP   PROTEIN SEQUENCE OF 13-107.
RA   Chauvet M.-T., Chauvet J., Acher R.;
RT   "The neurohypophysial hormone-binding protein: complete amino-acid sequence
RT   of ovine and bovine MSEL-neurophysins.";
RL   Eur. J. Biochem. 69:475-485(1976).
RN   [3]
RP   PROTEIN SEQUENCE OF 13-107.
RX   PubMed=1236812; DOI=10.1016/0014-5793(75)80151-7;
RA   Schlesinger D.H., Ernst M., Nicholas A., Watkins W.B., Walter R.;
RT   "Complete amino acid sequence of ovine neurophysin-III.";
RL   FEBS Lett. 57:55-59(1975).
RN   [4]
RP   PROTEIN SEQUENCE OF 109-147.
RX   PubMed=465021; DOI=10.1016/s0006-291x(79)80007-8;
RA   Smyth D.G., Massey D.E.;
RT   "A new glycopeptide in pig, ox and sheep pituitary.";
RL   Biochem. Biophys. Res. Commun. 87:1006-1010(1979).
CC   -!- FUNCTION: Neurophysin 2 specifically binds vasopressin.
CC   -!- FUNCTION: Vasopressin has a direct antidiuretic action on the kidney,
CC       it also causes vasoconstriction of the peripheral vessels. Acts by
CC       binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and
CC       V2R/AVPR2) (By similarity). {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBUNIT: Interacts with vasopressin receptors V1bR/AVPR1B (Ki=85 pM),
CC       V1aR/AVPR1A (Ki=0.6 nM) and V2R/AVPR2 (Ki=4.9 nM) (By similarity).
CC       Interacts with oxytocin receptor (OXTR) (Ki=110 nM) (By similarity).
CC       {ECO:0000250|UniProtKB:P01185}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MISCELLANEOUS: X's have been placed at positions 10-12 and 108 by
CC       homology with the complete sequence of the bovine precursor.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR   PIR; B90737; NVSH2.
DR   Proteomes; UP000002356; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   GO; GO:0042310; P:vasoconstriction; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.9.10; -; 1.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR   InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR   PANTHER; PTHR11681; PTHR11681; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   SMART; SM00003; NH; 1.
DR   SUPFAM; SSF49606; SSF49606; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hormone; Reference proteome; Secreted;
KW   Vasoactive; Vasoconstrictor.
FT   PEPTIDE         1..9
FT                   /note="Arg-vasopressin"
FT                   /id="PRO_0000020527"
FT   CHAIN           13..107
FT                   /note="Neurophysin 2"
FT                   /id="PRO_0000020528"
FT   PEPTIDE         109..147
FT                   /note="Copeptin"
FT                   /id="PRO_0000020529"
FT   SITE            9
FT                   /note="Important for agonist activity on V1aR/AVPR1A"
FT                   /evidence="ECO:0000250|UniProtKB:P01185"
FT   MOD_RES         9
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000269|Ref.1"
FT   CARBOHYD        114
FT                   /note="N-linked (GlcNAc...) asparagine"
FT   DISULFID        1..6
FT   DISULFID        22..66
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        25..39
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        33..56
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        40..46
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        73..85
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        79..97
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        86..91
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   CONFLICT        105
FT                   /note="Missing (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
SQ   SEQUENCE   147 AA;  15321 MW;  9C41B3288200E50C CRC64;
     CYFQNCPRGX XXAMSDLELR QCLPCGPGGK GRCFGPSICC GDELGCFVGT AEALRCQEEI
     YLPSPCQSGQ KPCGSGGRCA AAGICCNDES CVTEPECREG IGFPRRVXAS DRSNATLLDG
     PSGALLLRLV QLAAAPEPAE PAQPGVY