ID   NEUA_NEIME              Reviewed;         228 AA.
AC   P0A0Z8; Q57385;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=N-acylneuraminate cytidylyltransferase;
DE            EC=2.7.7.43;
DE   AltName: Full=CMP-N-acetylneuraminic acid synthase;
DE            Short=CMP-NeuNAc synthase;
DE   AltName: Full=CMP-sialic acid synthase;
GN   Name=neuA; Synonyms=siaB, synB;
OS   Neisseria meningitidis.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=487;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRCC 4030 / 406Y / Serogroup Y;
RA   Gilbert M., Watson D.C., Wakarchuk W.W.;
RT   "Purification and characterization of the recombinant CMP-sialic acid
RT   synthetase from Neisseria meningitidis.";
RL   Biotechnol. Lett. 19:417-420(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an N-acylneuraminate + CTP = a CMP-N-acyl-beta-neuraminate +
CC         diphosphate; Xref=Rhea:RHEA:11344, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:37563, ChEBI:CHEBI:60073, ChEBI:CHEBI:68671; EC=2.7.7.43;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the CMP-NeuNAc synthase family. {ECO:0000305}.
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DR   EMBL; U60146; AAB60780.1; -; Genomic_DNA.
DR   RefSeq; WP_002215295.1; NZ_WSOH01000068.1.
DR   PDB; 1EYR; X-ray; 2.20 A; A/B=1-228.
DR   PDB; 1EZI; X-ray; 2.00 A; A/B=1-228.
DR   PDB; 6CKJ; X-ray; 1.75 A; A=1-228.
DR   PDB; 6CKK; X-ray; 1.80 A; A/B=1-228.
DR   PDB; 6CKL; X-ray; 2.68 A; A/B/C=1-228.
DR   PDB; 6CKM; X-ray; 1.54 A; A=1-228.
DR   PDBsum; 1EYR; -.
DR   PDBsum; 1EZI; -.
DR   PDBsum; 6CKJ; -.
DR   PDBsum; 6CKK; -.
DR   PDBsum; 6CKL; -.
DR   PDBsum; 6CKM; -.
DR   AlphaFoldDB; P0A0Z8; -.
DR   SMR; P0A0Z8; -.
DR   DrugBank; DB04555; Cytidine-5'-Diphosphate.
DR   GeneID; 61282335; -.
DR   OMA; AYHMKEL; -.
DR   BRENDA; 2.7.7.43; 3593.
DR   EvolutionaryTrace; P0A0Z8; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008781; F:N-acylneuraminate cytidylyltransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR003329; Cytidylyl_trans.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF02348; CTP_transf_3; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..228
FT                   /note="N-acylneuraminate cytidylyltransferase"
FT                   /id="PRO_0000213206"
FT   STRAND          4..10
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          16..18
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           21..23
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           31..42
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          46..53
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           55..63
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          67..70
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           73..75
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           82..93
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          97..102
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           112..119
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   TURN            124..126
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          130..135
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          144..148
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          151..156
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           158..162
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           165..167
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          172..182
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           183..189
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   STRAND          198..201
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           205..207
FT                   /evidence="ECO:0007829|PDB:6CKM"
FT   HELIX           213..223
FT                   /evidence="ECO:0007829|PDB:6CKM"
SQ   SEQUENCE   228 AA;  24892 MW;  F2510733EE1C3A31 CRC64;
     MEKQNIAVIL ARQNSKGLPL KNLRKMNGIS LLGHTINAAI SSKCFDRIIV STDGGLIAEE
     AKNFGVEVVL RPAELASDTA SSISGVIHAL ETIGSNSGTV TLLQPTSPLR TGAHIREAFS
     LFDEKIKGSV VSACPMEHHP LKTLLQINNG EYAPMRHLSD LEQPRQQLPQ AFRPNGAIYI
     NDTASLIANN CFFIAPTKLY IMSHQDSIDI DTELDLQQAE NILNHKES