ID   NEUFC_DROME             Reviewed;         287 AA.
AC   Q9W376;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Neuferricin homolog;
DE   AltName: Full=Cytochrome b5 domain-containing protein 2 homolog;
DE   Flags: Precursor;
GN   ORFNames=CG12056;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- FUNCTION: Heme-binding protein. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- DOMAIN: The cytochrome b5 heme-binding domain was proven to bind heme,
CC       although it lacks the conserved iron-binding His residues at position
CC       99 and 126. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cytochrome b5 family. MAPR subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE014298; AAF46459.1; -; Genomic_DNA.
DR   EMBL; AY069225; AAL39370.1; -; mRNA.
DR   RefSeq; NP_572535.1; NM_132307.4.
DR   AlphaFoldDB; Q9W376; -.
DR   SMR; Q9W376; -.
DR   BioGRID; 58308; 1.
DR   IntAct; Q9W376; 1.
DR   STRING; 7227.FBpp0071240; -.
DR   PaxDb; Q9W376; -.
DR   PRIDE; Q9W376; -.
DR   DNASU; 31855; -.
DR   EnsemblMetazoa; FBtr0071305; FBpp0071240; FBgn0030099.
DR   GeneID; 31855; -.
DR   KEGG; dme:Dmel_CG12056; -.
DR   UCSC; CG12056-RA; d. melanogaster.
DR   FlyBase; FBgn0030099; CG12056.
DR   VEuPathDB; VectorBase:FBgn0030099; -.
DR   eggNOG; KOG1108; Eukaryota.
DR   GeneTree; ENSGT00940000160156; -.
DR   HOGENOM; CLU_065455_0_1_1; -.
DR   InParanoid; Q9W376; -.
DR   OMA; GHKHYGP; -.
DR   OrthoDB; 1355837at2759; -.
DR   PhylomeDB; Q9W376; -.
DR   BioGRID-ORCS; 31855; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 31855; -.
DR   PRO; PR:Q9W376; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0030099; Expressed in seminal fluid secreting gland and 12 other tissues.
DR   Genevisible; Q9W376; DM.
DR   GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   Gene3D; 3.10.120.10; -; 1.
DR   InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR   InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR   Pfam; PF00173; Cyt-b5; 1.
DR   SMART; SM01117; Cyt-b5; 1.
DR   SUPFAM; SSF55856; SSF55856; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..287
FT                   /note="Neuferricin homolog"
FT                   /id="PRO_0000312328"
FT   DOMAIN          61..146
FT                   /note="Cytochrome b5 heme-binding"
FT   COILED          175..204
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   287 AA;  32628 MW;  1D7F9087C9C09FA1 CRC64;
     MFGLLRHLFK FQFLFVVAAV LGGIYHTEIR QFLRRQTDNY LDQAGQDASI PLAFQAGDDI
     GTLFTPAELA KFNGEEEGRP LYLALLGSVF DVSRGIKHYG SGCSYNFFVG RDASVSFISG
     DFETYDPETA DDVLTLKPDD LIGLAGWRDF YQKDYVYKGR VIGRFYDEKG ALTTYHHKFL
     ELLEQARDAK RQVEELRARY PGCNIEWSEE RGTRVWCTTT SGDGKERSWI GYPRKLYSRG
     NKSFQCACVP DAELDEIDAG GKVAHGDAML KPYDNCEPQA RECFYRV