NEUL4_MOUSE
ID NEUL4_MOUSE Reviewed; 1563 AA.
AC Q5NCX5; Q3U090; Q5NCX4; Q69ZA2; Q8R1V5;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Neuralized-like protein 4;
GN Name=Neurl4; Synonyms=Kiaa1787;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 19-1563 (ISOFORM 1).
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 142-1563 (ISOFORM 2).
RC TISSUE=Embryo;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 936-1563.
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Promotes CCP110 ubiquitination and proteasome-dependent
CC degradation. By counteracting accumulation of CP110, maintains normal
CC centriolar homeostasis and preventing formation of ectopic microtubular
CC organizing centers (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CCP110; this interaction propmotes CCP110
CC ubiquitination and degradation via the proteasome pathway. Via its
CC interaction with CCP110, may indirectly interact with CEP97. Interacts
CC with the E3 ubiquitin-protein ligase HERC2 and UBE3A. May interact with
CC MAPK6 and hence mediate MAPK6 interaction with UBE3A. Interaction with
CC UBE3A may be indirect and mediated by HERC2 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5NCX5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5NCX5-2; Sequence=VSP_027564;
CC -!- PTM: Ubiquitinated; undergoes HERC2-dependent 'Lys-48' ubiquitination.
CC This ubiquitination leads to proteasomal degradation (By similarity).
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI35150.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL596185; CAI35149.1; -; Genomic_DNA.
DR EMBL; AL596185; CAI35150.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK157107; BAE33965.1; -; mRNA.
DR EMBL; AK173264; BAD32542.1; -; mRNA.
DR EMBL; BC023037; AAH23037.1; -; mRNA.
DR CCDS; CCDS24921.1; -. [Q5NCX5-1]
DR RefSeq; NP_001013432.1; NM_001013414.3. [Q5NCX5-1]
DR RefSeq; NP_001278047.1; NM_001291118.1.
DR RefSeq; NP_001278048.1; NM_001291119.1.
DR RefSeq; XP_017169947.1; XM_017314458.1. [Q5NCX5-2]
DR AlphaFoldDB; Q5NCX5; -.
DR SMR; Q5NCX5; -.
DR BioGRID; 229809; 8.
DR IntAct; Q5NCX5; 4.
DR MINT; Q5NCX5; -.
DR STRING; 10090.ENSMUSP00000053235; -.
DR iPTMnet; Q5NCX5; -.
DR PhosphoSitePlus; Q5NCX5; -.
DR MaxQB; Q5NCX5; -.
DR PaxDb; Q5NCX5; -.
DR PRIDE; Q5NCX5; -.
DR ProteomicsDB; 287386; -. [Q5NCX5-1]
DR ProteomicsDB; 287387; -. [Q5NCX5-2]
DR Antibodypedia; 48206; 15 antibodies from 7 providers.
DR Ensembl; ENSMUST00000061837; ENSMUSP00000053235; ENSMUSG00000047284. [Q5NCX5-1]
DR Ensembl; ENSMUST00000108617; ENSMUSP00000104257; ENSMUSG00000047284. [Q5NCX5-2]
DR GeneID; 216860; -.
DR KEGG; mmu:216860; -.
DR UCSC; uc007jsn.3; mouse. [Q5NCX5-1]
DR UCSC; uc011xxf.2; mouse. [Q5NCX5-2]
DR CTD; 84461; -.
DR MGI; MGI:1921092; Neurl4.
DR VEuPathDB; HostDB:ENSMUSG00000047284; -.
DR eggNOG; KOG4625; Eukaryota.
DR GeneTree; ENSGT00940000159866; -.
DR HOGENOM; CLU_002040_1_0_1; -.
DR InParanoid; Q5NCX5; -.
DR OMA; TTHNPAV; -.
DR OrthoDB; 74097at2759; -.
DR PhylomeDB; Q5NCX5; -.
DR BioGRID-ORCS; 216860; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Neurl4; mouse.
DR PRO; PR:Q5NCX5; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5NCX5; protein.
DR Bgee; ENSMUSG00000047284; Expressed in retinal neural layer and 252 other tissues.
DR ExpressionAtlas; Q5NCX5; baseline and differential.
DR Genevisible; Q5NCX5; MM.
DR GO; GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR Gene3D; 2.60.120.920; -; 6.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR037962; Neuralized.
DR InterPro; IPR006573; NHR_dom.
DR PANTHER; PTHR12429; PTHR12429; 1.
DR Pfam; PF07177; Neuralized; 6.
DR SMART; SM00588; NEUZ; 6.
DR SUPFAM; SSF49899; SSF49899; 2.
DR PROSITE; PS51065; NHR; 6.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway.
FT CHAIN 1..1563
FT /note="Neuralized-like protein 4"
FT /id="PRO_0000299106"
FT DOMAIN 43..209
FT /note="NHR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT DOMAIN 319..486
FT /note="NHR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT DOMAIN 522..688
FT /note="NHR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT DOMAIN 718..886
FT /note="NHR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT DOMAIN 915..1087
FT /note="NHR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT DOMAIN 1132..1295
FT /note="NHR 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 692..717
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1087..1121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 699..716
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1090..1105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 504
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96JN8"
FT MOD_RES 909
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96JN8"
FT VAR_SEQ 245..266
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15368895"
FT /id="VSP_027564"
FT CONFLICT 1247
FT /note="L -> M (in Ref. 2; BAE33965)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1563 AA; 167637 MW; 65F86EC95429B6C0 CRC64;
MAAGSGGSGG SGAGPGPGPG PGGGGGPGSS GPGLGSGGGL GGGGELHPRT GRLVSLSACG
RTARRQQPGQ EFNHGLVLSR EPLRDGRVFT VRIDRKVNSW SGSIEIGVTA LDPSVLDFPS
SATGLKGGSW VVSGCSVLRD GRSVLEEYGQ DLDQLVEGDR VGVERTATGE LRLWVNGRDC
GVAATGLPAR VWAVVDLYGK CTQITVLPSE PGFSPPTPVP TPPLEPLAPP EDSALLEQGT
SVDEAFMVSP AQARPETFPN SLDSHNDFAS MELSEVVSNA ILSAYNGGLL NVSLSSPPAG
DGLASSGPAT SPILTSNDAL LFHEKCGTLI KLSNNNKTAE RRRPLDEFNN GVVMTNRPLR
DNEMFEIRID KLVDKWSGSI EIGVTTHNPN SLEYPATMTN LQSGTIMMSG CGILTNGKGT
RREYCEFSLD ELQEGDHIGL TRKSNSALHF FINGIDQGVA TPLTPPVVYG VVDLYGMAVK
VTIVHNNNHS DRLRRNNAIL RALSPEGALR RAAPAAQAEP ERLLFHPNCG QKAAITHEGR
TALRPHATDD FNHGVVLSSR ALRDGEVFQV RIDKMVDKWA GSIEIGVTTH NPAYLQLPST
MTNLRSGTWM MTGNGVMHNG TTILDEYGHN LDRLKAGDTV GVVRREDGTL HFFVNGMTQG
PAAWNVPPGV YAVVDLYGQA AQATIVDDVE VPPVSEPLPE GNNQMSPSSP SSAAGGSDLR
FHQLHGSNAV ITNGGRTALR HNCRSEFNDA IVISNRALRD GELFEIVIQK MVDRWSGSIE
AGVTAIRPED LEFPNTMTDI DYDTWMLSGT AIMQDGNTMR NNYGCDLDAL GTGARIGMMR
TAKGDLHYFI NGQDQGAACS GLPPGKEVYA VVDLYGQCVQ VSITNATGPM DNSLATSNTA
TEKSFPLHSP VAGVAHRFHS MCGKNVTLEE DGTRAVRVAG YAHGLVFSTK ELKAEEVFEV
KVEELDEKWA GSLRLGLTTL APEDMGPGAG SGPGLPPSLP ELRTKTTWMV SSCEVRRDGH
LQRMNYGRNL ERLGVGSRVG IRRCADDTMH ILVDGEDMGP AAAGIAKNVW AVLDLYGPVR
SVAIVSSTRL EEPEGTQPPS PSSDTGSEVE EDDEVEEQGL RGQNQVGIVP TALEFLENHG
KNILLSNGNR TATRVASYNQ GIVVISQPLV PHMLVQVRID FLNRQWTSSL VLGVITCPPE
RLNFPASACA LKRAAWLLRG RGVFHNGLKI CEKFGPNLDT CPEGTILGLR LDSSGGLHLH
INGVDQGVAV PDVPQPCHAL VDLYGQCEQV TIVSPDPGTA SGKIAGTQGD MEKADMVDGI
KESVCWGPPP AASPLKSCEY HALCSRFQEL LLLPEDYFMP PPKRSLCYCE SCRKLRGDEA
HRRRGEPPRE YALPFGWCRF NLRVNPHLEA GTLTKKWHMA YHGSSVAVVR RVLDRGELGA
GTTSILSCRP LKGEPGVGFE EPGENCAPPR EEQPPPVLLS PSLQYAGAEM LASKVQFRDP
KSQRTHQAQV AFQVCVRPGS YTPGPPSAAL RELPDQHFSP SELEWVTKEK GATLLYALLV
RVE
