NEUM_CHICK
ID NEUM_CHICK Reviewed; 246 AA.
AC P35001;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 03-AUG-2022, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Neuromodulin;
DE AltName: Full=Axonal membrane protein GAP-43;
DE AltName: Full=Growth-associated protein 43;
GN Name=GAP43;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=2153895; DOI=10.1016/0169-328x(90)90074-n;
RA Baizer L., Alkan S., Stocker K., Ciment G.;
RT "Chicken growth-associated protein (GAP)-43: primary structure and
RT regulated expression of mRNA during embryogenesis.";
RL Brain Res. Mol. Brain Res. 7:61-68(1990).
RN [2]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND PALMITOYLATION.
RX PubMed=9813098; DOI=10.1083/jcb.143.3.795;
RA Kutzleb C., Sanders G., Yamamoto R., Wang X., Lichte B.,
RA Petrasch-Parwez E., Kilimann M.W.;
RT "Paralemmin, a prenyl-palmitoyl-anchored phosphoprotein abundant in neurons
RT and implicated in plasma membrane dynamics and cell process formation.";
RL J. Cell Biol. 143:795-813(1998).
CC -!- FUNCTION: This protein is associated with nerve growth. It is a major
CC component of the motile 'growth cones' that form the tips of elongating
CC axons. Plays a role in axonal and dendritic filopodia induction (By
CC similarity). {ECO:0000250|UniProtKB:P17677}.
CC -!- SUBUNIT: Binds calmodulin with a greater affinity in the absence of
CC Ca(2+) than in its presence. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9813098};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P17677}; Cytoplasmic
CC side {ECO:0000250|UniProtKB:P17677}. Cell projection, growth cone
CC membrane {ECO:0000250|UniProtKB:P17677}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P17677}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P17677}. Synapse {ECO:0000269|PubMed:9813098}.
CC Cell projection, filopodium membrane {ECO:0000269|PubMed:9813098};
CC Peripheral membrane protein {ECO:0000250|UniProtKB:P17677}.
CC -!- TISSUE SPECIFICITY: Expressed in neurons. {ECO:0000269|PubMed:9813098}.
CC -!- DEVELOPMENTAL STAGE: First expressed in the body and head at embryonic
CC stage E3 (PubMed:2153895). Expressed in the mid-thoracic region of
CC embryos and also in the spinal cord, dorsal root and sympathetic
CC ganglia at embryonic stage E10 (PubMed:2153895). Highly expressed in
CC the brain at embryonic stage E13 (PubMed:2153895). Expressed at low
CC levels in the gut at embryonic stage E16 (PubMed:2153895). Not
CC expressed in heart, skeletal muscle or liver at embryonic stage E16
CC (PubMed:2153895). {ECO:0000269|PubMed:2153895}.
CC -!- PTM: Palmitoylated (PubMed:9813098). Palmitoylation is essential for
CC plasma membrane association (PubMed:9813098).
CC {ECO:0000269|PubMed:9813098}.
CC -!- SIMILARITY: Belongs to the neuromodulin family. {ECO:0000305}.
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DR PIR; A60049; A60049.
DR AlphaFoldDB; P35001; -.
DR VEuPathDB; HostDB:geneid_427955; -.
DR eggNOG; ENOG502RXWF; Eukaryota.
DR InParanoid; P35001; -.
DR PhylomeDB; P35001; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:AgBase.
DR GO; GO:0031527; C:filopodium membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032584; C:growth cone membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044306; C:neuron projection terminus; IDA:AgBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0014069; C:postsynaptic density; IBA:GO_Central.
DR GO; GO:0097060; C:synaptic membrane; IDA:AgBase.
DR GO; GO:0005516; F:calmodulin binding; IBA:GO_Central.
DR GO; GO:0035727; F:lysophosphatidic acid binding; IBA:GO_Central.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; IBA:GO_Central.
DR GO; GO:0001786; F:phosphatidylserine binding; IBA:GO_Central.
DR GO; GO:0016198; P:axon choice point recognition; IBA:GO_Central.
DR GO; GO:0031103; P:axon regeneration; IBA:GO_Central.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0042246; P:tissue regeneration; IBA:GO_Central.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR001422; Neuromodulin.
DR InterPro; IPR017454; Neuromodulin_C.
DR InterPro; IPR018947; Neuromodulin_gap-junction_N.
DR InterPro; IPR033137; Neuromodulin_P_site.
DR InterPro; IPR018243; Neuromodulin_palmitoyl_site.
DR Pfam; PF00612; IQ; 1.
DR Pfam; PF06614; Neuromodulin; 1.
DR Pfam; PF10580; Neuromodulin_N; 1.
DR PRINTS; PR00215; NEUROMODULIN.
DR SMART; SM00015; IQ; 1.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS00412; NEUROMODULIN_1; 1.
DR PROSITE; PS00413; NEUROMODULIN_2; 1.
PE 1: Evidence at protein level;
KW Calmodulin-binding; Cell membrane; Cell projection; Developmental protein;
KW Differentiation; Growth regulation; Lipoprotein; Membrane; Neurogenesis;
KW Palmitate; Phosphoprotein; Reference proteome; Synapse.
FT CHAIN 1..246
FT /note="Neuromodulin"
FT /id="PRO_0000159600"
FT DOMAIN 32..61
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 1..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..246
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 3
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P06837"
FT LIPID 4
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P06837"
SQ SEQUENCE 246 AA; 25631 MW; 6F9330F121126F01 CRC64;
MLCCMRRTKQ VEKNEDGDQK IEQDGIKPED KAHKAATKIQ ASFRGHITRK KLKGEKKADA
PASESEAADK KDEGPAGGAA ENKESEASAA TEASAADSAQ LDEGSKDSSV PAEEKKGNGA
ADTGSEQPAP QAATPAASSE EKPAAAAETE SATKASTDNS PSLKADEAQD KEEPKQADVP
AADTTATTTP AAEDATAKAT AQPQMETVES SQTEEKTDAV EETKPTESAQ QEEVKEEESK
ADQENA
