NEUR_DROME
ID NEUR_DROME Reviewed; 754 AA.
AC P29503; Q26306; Q27273; Q8INP6; Q8INP7; Q960M1;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 190.
DE RecName: Full=Protein neuralized;
GN Name=neur; Synonyms=neu; ORFNames=CG11988;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
RC STRAIN=Oregon-R;
RX PubMed=1717258; DOI=10.1002/j.1460-2075.1991.tb07848.x;
RA Boulianne G.L., de la Concha A., Campos-Ortega J.A., Jan L.Y., Jan Y.N.;
RT "The Drosophila neurogenic gene neuralized encodes a novel protein and is
RT expressed in precursors of larval and adult neurons.";
RL EMBO J. 10:2975-2983(1991).
RN [2]
RP ERRATUM OF PUBMED:1717258.
RX PubMed=8508781; DOI=10.1002/j.1460-2075.1993.tb05914.x;
RA Boulianne G.L., de la Concha A., Campos-Ortega J.A., Jan L.Y., Jan Y.N.;
RL EMBO J. 12:2586-2586(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8508767; DOI=10.1002/j.1460-2075.1993.tb05895.x;
RA Price B.D., Chang Z., Smith R., Bockheim S., Laughon A.;
RT "The Drosophila neuralized gene encodes a C3HC4 zinc finger.";
RL EMBO J. 12:2411-2418(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=Berkeley;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338 AND SER-341, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [8]
RP STRUCTURE BY NMR OF 106-266.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the neuz (NHR) domain in neuralized from Drosophila
RT melanogaster.";
RL Submitted (OCT-2007) to the PDB data bank.
CC -!- FUNCTION: Involved in neurogenesis. Interacts with other neurogenic
CC proteins in the specification of the neuroblast versus epidermoblast
CC cell fate.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=P29503-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P29503-2; Sequence=VSP_008046;
CC Name=3;
CC IsoId=P29503-3; Sequence=VSP_008045;
CC Name=4;
CC IsoId=P29503-4; Sequence=VSP_008045, VSP_008046;
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA43806.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X61617; CAA43806.1; ALT_FRAME; Genomic_DNA.
DR EMBL; S62597; AAB27151.1; -; mRNA.
DR EMBL; S62583; AAB27147.1; -; mRNA.
DR EMBL; L12218; AAA28403.1; -; mRNA.
DR EMBL; AE014297; AAF54330.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54326.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13406.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13407.1; -; Genomic_DNA.
DR EMBL; AY051987; AAK93411.1; -; mRNA.
DR EMBL; BT003772; AAO41451.1; -; mRNA.
DR PIR; S35371; S35371.
DR PIR; S35503; S35503.
DR RefSeq; NP_001163563.1; NM_001170092.3. [P29503-3]
DR RefSeq; NP_476652.1; NM_057304.5. [P29503-1]
DR RefSeq; NP_731309.1; NM_169255.3. [P29503-4]
DR RefSeq; NP_731310.1; NM_169256.3. [P29503-3]
DR RefSeq; NP_731311.1; NM_169257.3. [P29503-2]
DR PDB; 2YUE; NMR; -; A=106-266.
DR PDB; 4KG0; X-ray; 2.10 A; A=105-260.
DR PDBsum; 2YUE; -.
DR PDBsum; 4KG0; -.
DR AlphaFoldDB; P29503; -.
DR SMR; P29503; -.
DR BioGRID; 66253; 29.
DR DIP; DIP-17176N; -.
DR IntAct; P29503; 4.
DR MINT; P29503; -.
DR STRING; 7227.FBpp0081481; -.
DR iPTMnet; P29503; -.
DR PaxDb; P29503; -.
DR DNASU; 41085; -.
DR EnsemblMetazoa; FBtr0082001; FBpp0081479; FBgn0002932. [P29503-3]
DR EnsemblMetazoa; FBtr0082002; FBpp0081480; FBgn0002932. [P29503-4]
DR EnsemblMetazoa; FBtr0082003; FBpp0081481; FBgn0002932. [P29503-1]
DR EnsemblMetazoa; FBtr0082004; FBpp0081482; FBgn0002932. [P29503-2]
DR EnsemblMetazoa; FBtr0300414; FBpp0289643; FBgn0002932. [P29503-3]
DR GeneID; 41085; -.
DR KEGG; dme:Dmel_CG11988; -.
DR UCSC; CG11988-RA; d. melanogaster. [P29503-1]
DR CTD; 41085; -.
DR FlyBase; FBgn0002932; neur.
DR VEuPathDB; VectorBase:FBgn0002932; -.
DR eggNOG; KOG4172; Eukaryota.
DR eggNOG; KOG4625; Eukaryota.
DR GeneTree; ENSGT00940000166233; -.
DR InParanoid; P29503; -.
DR OMA; AMCPIPQ; -.
DR PhylomeDB; P29503; -.
DR SignaLink; P29503; -.
DR BioGRID-ORCS; 41085; 0 hits in 3 CRISPR screens.
DR EvolutionaryTrace; P29503; -.
DR GenomeRNAi; 41085; -.
DR PRO; PR:P29503; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0002932; Expressed in mesectoderm and 85 other tissues.
DR ExpressionAtlas; P29503; baseline and differential.
DR Genevisible; P29503; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; IDA:FlyBase.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:FlyBase.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; TAS:Reactome.
DR GO; GO:0008270; F:zinc ion binding; ISM:FlyBase.
DR GO; GO:0008356; P:asymmetric cell division; IMP:FlyBase.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0030718; P:germ-line stem cell population maintenance; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0007616; P:long-term memory; IMP:FlyBase.
DR GO; GO:0007498; P:mesoderm development; IMP:FlyBase.
DR GO; GO:0035204; P:negative regulation of lamellocyte differentiation; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IMP:FlyBase.
DR GO; GO:0007400; P:neuroblast fate determination; IMP:FlyBase.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:FlyBase.
DR GO; GO:0045807; P:positive regulation of endocytosis; IMP:FlyBase.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0008104; P:protein localization; IMP:FlyBase.
DR GO; GO:0000209; P:protein polyubiquitination; IDA:FlyBase.
DR GO; GO:0045314; P:regulation of compound eye photoreceptor development; IMP:FlyBase.
DR GO; GO:0046532; P:regulation of photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR GO; GO:0016360; P:sensory organ precursor cell fate determination; IDA:FlyBase.
DR GO; GO:0007419; P:ventral cord development; HMP:FlyBase.
DR Gene3D; 2.60.120.920; -; 2.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR037962; Neuralized.
DR InterPro; IPR006573; NHR_dom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR12429; PTHR12429; 1.
DR Pfam; PF07177; Neuralized; 2.
DR SMART; SM00588; NEUZ; 2.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS51065; NHR; 2.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; Differentiation;
KW DNA-binding; Metal-binding; Neurogenesis; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..754
FT /note="Protein neuralized"
FT /id="PRO_0000055975"
FT DOMAIN 106..260
FT /note="NHR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT DOMAIN 368..523
FT /note="NHR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00400"
FT ZN_FING 701..742
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 280..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 338
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 341
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..90
FT /note="MGLSDIPANYMQGSHPHLTLHPQQQHHQNQQHLQHLQQMQQLHNAMPTPAQQ
FT AAQVLAMESNELLMSTKDKLSSKKKMHLLKKIKKRFGL -> MGQSAGKI (in
FT isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_008045"
FT VAR_SEQ 672
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:12537569,
FT ECO:0000303|PubMed:1717258"
FT /id="VSP_008046"
FT CONFLICT 234
FT /note="T -> S (in Ref. 1; CAA43806)"
FT /evidence="ECO:0000305"
FT CONFLICT 270
FT /note="A -> V (in Ref. 3; AAB27151)"
FT /evidence="ECO:0000305"
FT CONFLICT 588
FT /note="T -> N (in Ref. 3; AAB27151)"
FT /evidence="ECO:0000305"
FT CONFLICT 694
FT /note="S -> R (in Ref. 3; AAB27151)"
FT /evidence="ECO:0000305"
FT STRAND 109..113
FT /evidence="ECO:0007829|PDB:2YUE"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 130..133
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 136..141
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 149..156
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 158..162
FT /evidence="ECO:0007829|PDB:2YUE"
FT STRAND 165..171
FT /evidence="ECO:0007829|PDB:4KG0"
FT HELIX 173..176
FT /evidence="ECO:0007829|PDB:4KG0"
FT HELIX 184..188
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 194..198
FT /evidence="ECO:0007829|PDB:4KG0"
FT HELIX 201..203
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 209..214
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 220..224
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 227..233
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 242..247
FT /evidence="ECO:0007829|PDB:4KG0"
FT STRAND 250..257
FT /evidence="ECO:0007829|PDB:4KG0"
FT HELIX 260..263
FT /evidence="ECO:0007829|PDB:2YUE"
SQ SEQUENCE 754 AA; 82325 MW; A2887404502D70BF CRC64;
MGLSDIPANY MQGSHPHLTL HPQQQHHQNQ QHLQHLQQMQ QLHNAMPTPA QQAAQVLAME
SNELLMSTKD KLSSKKKMHL LKKIKKRFGL VRRSPSSCPG PNNLPPLQFH SVHGDNIRIS
RDGTLARRFE SFCRAITFSA RPVRINERIC VKFAEISNNW NGGIRFGFTS NDPVTLEGTL
PKYACPDLTN RPGFWAKALH EQYCEKDNIL YYYVNGAGDV IYGINNEEKG VILTGIDTRS
LLWTVIDIYG NCTGIEFLDS RIYMYQQQPA AIPMATVPAQ QQQMPQPAAN ASSALNSHHP
HQQSRRSLPG HTAAIEHDLE RHVMPSLQSL HLAGNGGSVA SVEQAAIAHD LANGLPPLRY
NANGRLIPVP FHNTKGRNVR LSQDRFVASR TESDFCQGYV FTARPIRIGE KLIVQVLKTE
QMYVGALALG LTSCNPAMLQ PNDLPNDSDF LLDRPEYWVV SKDIAAAPQR GDEIAFFVAP
NGEVSISKNN GPAVVVMHVD QSLQLWAFLD VYGSTQSLRM FRQQLPNMVA YPSQPQVNVN
ASSSSACNAA STSRMLPMTE SMSSLNAGAT AKLLHHPSQL SVAQSTSTLA SAGGVNGSRM
ISMPSNGDIL QIQPNGGGTV LVVNLPPASS SHDINGQLAA RPTATVTSSG VLAGACSSGT
LISTTSSQYI EQPIANSTNN AANKWKDSLS DQQSTDSSAE CTICYENPID SVLYMCGHMC
MCYDCAIEQW RGVGGGQCPL CRAVIRDVIR TYTT
