ID   NEUV_PELLE              Reviewed;         141 AA.
AC   P11858;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Vasotocin-neurophysin VT;
DE            Short=VT;
DE   Contains:
DE     RecName: Full=Hydrin-2;
DE     AltName: Full=Hydrin II;
DE   Contains:
DE     RecName: Full=Vasotocin;
DE   Contains:
DE     RecName: Full=Neurophysin VT;
DE   Flags: Precursor;
OS   Pelophylax lessonae (Pool frog) (Rana lessonae).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Pelophylax.
OX   NCBI_TaxID=45623;
RN   [1]
RP   PROTEIN SEQUENCE, AND AMIDATION AT GLY-9.
RX   PubMed=3501288; DOI=10.1016/0006-291x(87)90401-3;
RA   Michel G., Chauvet J., Chauvet M.-T., Acher R.;
RT   "One-step processing of the amphibian vasotocin precursor: structure of a
RT   frog (Rana esculenta) 'big' neurophysin.";
RL   Biochem. Biophys. Res. Commun. 149:538-544(1987).
RN   [2]
RP   PROTEIN SEQUENCE OF 13-141.
RX   PubMed=3258254; DOI=10.1016/0014-5793(88)80645-8;
RA   Chauvet J., Michel G., Chauvet M.-T., Acher R.;
RT   "An amphibian two-domain 'big' neurophysin: conformational homology with
RT   the mammalian MSEL-neurophysin/copeptin intermediate precursor shown by
RT   trypsin-sepharose proteolysis.";
RL   FEBS Lett. 230:77-80(1988).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-10.
RX   PubMed=2787509; DOI=10.1073/pnas.86.14.5272;
RA   Rouille Y., Michel G., Chauvet M.-T., Chauvet J., Acher R.;
RT   "Hydrins, hydroosmotic neurohypophysial peptides: osmoregulatory adaptation
RT   in amphibians through vasotocin precursor processing.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:5272-5275(1989).
CC   -!- FUNCTION: Vasotocin is an antidiuretic hormone.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Seven disulfide bonds are present in neurophysin.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family. {ECO:0000305}.
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DR   PIR; A27482; A27482.
DR   PIR; A33900; A33900.
DR   AlphaFoldDB; P11858; -.
DR   SMR; P11858; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   Gene3D; 2.60.9.10; -; 1.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR036387; Neurhyp_horm_dom_sf.
DR   InterPro; IPR022423; Neurohypophysial_hormone_CS.
DR   PANTHER; PTHR11681; PTHR11681; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   SMART; SM00003; NH; 1.
DR   SUPFAM; SSF49606; SSF49606; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Hormone; Secreted.
FT   PEPTIDE         1..10
FT                   /note="Hydrin-2"
FT                   /id="PRO_0000020541"
FT   PEPTIDE         1..9
FT                   /note="Vasotocin"
FT                   /id="PRO_0000020542"
FT   CHAIN           13..141
FT                   /note="Neurophysin VT"
FT                   /id="PRO_0000020543"
FT   MOD_RES         9
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000269|PubMed:3501288"
FT   CARBOHYD        117
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000305"
FT   DISULFID        1..6
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        22..66
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        25..39
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        33..56
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        40..46
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        73..85
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        79..97
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
FT   DISULFID        86..91
FT                   /evidence="ECO:0000250|UniProtKB:P01175"
SQ   SEQUENCE   141 AA;  15322 MW;  0142C4C7880E954F CRC64;
     CYIQNCPRGG KRSYPDTEVR QCIPCGPGNR GNCFGPNICC GEDLGCYIGT PETLRCVEEN
     YLPSPCEAGG KPCGAGGRCA APGVCCNDQS CTMDSSCLDE DSERQRVSPD QNMTQMNGSA
     SDLLLRLMHM ANRQQQQTKH Y