NEXMI_MOUSE
ID NEXMI_MOUSE Reviewed; 1515 AA.
AC Q5DTT1; Q003Y8; Q8BW26; Q8BYR0;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Neurite extension and migration factor {ECO:0000250|UniProtKB:Q5QGS0};
DE AltName: Full=KIAA2022 protein associated with intellectual disability, language impairment and autistic behavior homolog {ECO:0000303|PubMed:27822498};
DE Short=KIDLIA {ECO:0000303|PubMed:27822498};
GN Name=Nexmif {ECO:0000250|UniProtKB:Q5QGS0};
GN Synonyms=Kiaa2022 {ECO:0000303|Ref.2};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND DEVELOPMENTAL STAGE.
RC STRAIN=SWR/J; TISSUE=Embryo;
RX PubMed=19524067; DOI=10.1016/j.gep.2009.06.001;
RA Cantagrel V., Haddad M.R., Ciofi P., Andrieu D., Lossi A.M., Maldergem L.,
RA Roux J.C., Villard L.;
RT "Spatiotemporal expression in mouse brain of Kiaa2022, a gene disrupted in
RT two patients with severe mental retardation.";
RL Gene Expr. Patterns 9:423-429(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-730.
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus, and Ovary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=22531377; DOI=10.1016/j.neuroscience.2012.04.030;
RA Ishikawa T., Miyata S., Koyama Y., Yoshikawa K., Hattori T., Kumamoto N.,
RA Shingaki K., Katayama T., Tohyama M.;
RT "Transient expression of Xpn, an XLMR protein related to neurite extension,
RT during brain development and participation in neurite outgrowth.";
RL Neuroscience 214:181-191(2012).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=27822498; DOI=10.1523/eneuro.0238-16.2016;
RA Gilbert J., Man H.Y.;
RT "The X-linked autism protein KIAA2022/KIDLIA regulates neurite outgrowth
RT via N-cadherin and delta-catenin signaling.";
RL ENeuro 3:0-0(2016).
CC -!- FUNCTION: Involved in neurite outgrowth by regulating cell-cell
CC adhesion via the N-cadherin signaling pathway. May act by regulating
CC expression of protein-coding genes, such as N-cadherins and integrin
CC beta-1 (ITGB1). {ECO:0000269|PubMed:27822498}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22531377,
CC ECO:0000269|PubMed:27822498}. Cytoplasm {ECO:0000269|PubMed:22531377}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5DTT1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5DTT1-2; Sequence=VSP_021372;
CC -!- TISSUE SPECIFICITY: Expressed in the brain, particularly during the
CC late embryonic and perinatal stages of development (PubMed:22531377).
CC In the developing brain, it is expressed only in the cortical plate and
CC subplate region but not in the intermediate or ventricular zone
CC (PubMed:27822498). {ECO:0000269|PubMed:22531377,
CC ECO:0000269|PubMed:27822498}.
CC -!- DEVELOPMENTAL STAGE: Widely expressed in brain by postmitotic neurons
CC from early development through early adulthood.
CC {ECO:0000269|PubMed:19524067}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD90269.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DQ975302; ABI97947.1; -; mRNA.
DR EMBL; AK220439; BAD90269.1; ALT_INIT; mRNA.
DR EMBL; AL671963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK038635; BAC30074.1; -; mRNA.
DR EMBL; AK054513; BAC35808.1; -; mRNA.
DR CCDS; CCDS53166.1; -. [Q5DTT1-1]
DR RefSeq; NP_001070822.2; NM_001077354.2. [Q5DTT1-1]
DR RefSeq; XP_006528094.1; XM_006528031.3. [Q5DTT1-1]
DR RefSeq; XP_006528097.1; XM_006528034.3. [Q5DTT1-1]
DR AlphaFoldDB; Q5DTT1; -.
DR BioGRID; 232796; 1.
DR STRING; 10090.ENSMUSP00000085187; -.
DR iPTMnet; Q5DTT1; -.
DR PhosphoSitePlus; Q5DTT1; -.
DR PaxDb; Q5DTT1; -.
DR PRIDE; Q5DTT1; -.
DR ProteomicsDB; 287485; -. [Q5DTT1-1]
DR ProteomicsDB; 287486; -. [Q5DTT1-2]
DR Antibodypedia; 405; 15 antibodies from 8 providers.
DR Ensembl; ENSMUST00000056502; ENSMUSP00000049716; ENSMUSG00000046449. [Q5DTT1-2]
DR Ensembl; ENSMUST00000087879; ENSMUSP00000085187; ENSMUSG00000046449. [Q5DTT1-1]
DR Ensembl; ENSMUST00000118314; ENSMUSP00000113625; ENSMUSG00000046449. [Q5DTT1-2]
DR GeneID; 245555; -.
DR KEGG; mmu:245555; -.
DR UCSC; uc009uab.3; mouse. [Q5DTT1-1]
DR CTD; 340533; -.
DR MGI; MGI:2148050; Nexmif.
DR VEuPathDB; HostDB:ENSMUSG00000046449; -.
DR eggNOG; ENOG502QUMY; Eukaryota.
DR GeneTree; ENSGT00940000159746; -.
DR HOGENOM; CLU_250004_0_0_1; -.
DR InParanoid; Q5DTT1; -.
DR OMA; MMEPGTV; -.
DR OrthoDB; 59480at2759; -.
DR PhylomeDB; Q5DTT1; -.
DR TreeFam; TF332248; -.
DR BioGRID-ORCS; 245555; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Nexmif; mouse.
DR PRO; PR:Q5DTT1; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q5DTT1; protein.
DR Bgee; ENSMUSG00000046449; Expressed in anterior amygdaloid area and 168 other tissues.
DR ExpressionAtlas; Q5DTT1; baseline and differential.
DR Genevisible; Q5DTT1; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0072686; C:mitotic spindle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0033629; P:negative regulation of cell adhesion mediated by integrin; ISO:MGI.
DR GO; GO:2000048; P:negative regulation of cell-cell adhesion mediated by cadherin; ISO:MGI.
DR GO; GO:0001953; P:negative regulation of cell-matrix adhesion; ISO:MGI.
DR GO; GO:2001223; P:negative regulation of neuron migration; ISO:MGI.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR InterPro; IPR032757; DUF4683.
DR InterPro; IPR042794; Nexmif.
DR PANTHER; PTHR46946; PTHR46946; 1.
DR Pfam; PF15735; DUF4683; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Developmental protein; Neurogenesis;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1515
FT /note="Neurite extension and migration factor"
FT /id="PRO_0000257845"
FT REGION 381..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 505..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..774
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1158..1225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1372..1422
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1435..1479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 381..407
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 742..774
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1158..1197
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1375..1397
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1452..1479
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1486..1515
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_021372"
FT CONFLICT 451
FT /note="Y -> H (in Ref. 2; BAD90269)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1515 AA; 167102 MW; ABCF095AF2790870 CRC64;
MDNQQDKVIA ASANGDNNLI NGVKNNDSED QEVAMKSFVA LEATTPIQPI PVIQKESPMF
PRGLLPPPSK KPCMQSPPSP LALIEAPDHS ANSASVNAIS LTSGVAKGLN TWSLPNECEK
APFAIMEPAG MSALNGDCLM QPSRTCLGCF MESKEAVDPE PGISLKVSDL NRDYETCAVS
DIGIQCINAG ENIKYGEQLL SDQLLGFPLH KSRAGDRRES EKPDIDLEDP TQKSYYEALL
LDKCNTEEAL LANSNQDWGY FETFISESKI ELLDLCSKNE LSVNLFSEED VENYMFDDDE
STLGSDVCSL KIRYESFQDN VRDKTTLLMQ EDAQFNFFPS VFTTCPKRES KSGILKQSSD
LSQFKVPDVS IIWGEEDKNL DKKKGKEEVH EDKSIETKDE KDNGEKPALN NKPCGGLEVE
QFKNLKADQL TNSLETSGNF SDDSSFIEVS YDAMGEIKDC SRYMARDTNS GSSSSQQNYG
LRAKRKVRYS EDYLYDVDSL EGEKVNERKE WPPGGSKEED DDEWCPKKRR KVTRKEPPVI
IKYIIINRFK GEKNMLVKLS KVDASETTVN LSENQLSKYA KLSPLKGFWQ KKKKQKNSNT
DSVKTPLCQK QSFEPGSFEV SFLPPARKRK SKLGNRHRIQ RIQSVETSAS SKQVSFCSDQ
KQACNRKEDG VKGTPKSALL TDPSCANGSH LRGLIVSDSV KVKAQDTEFK GPERKVLNKI
KFKSEARLKS KKIKAGQENK PVVQMSPVSE DTSSKANLKN EVTPGTSNSS HMSEFHETKV
KNSTFLPTTC SSEMPLSSAN VATNIPVIPG GYLQTLLDAS DLSNNTSISY FTNHSAEQNE
GSLTQTEKAF VPLQSAQDCV LSSSSDSQLQ QSSQNFKMEA SNFGSLWPDK DTSGSQEFMT
EVSREIATNQ SSEFEASQVV SMENNLTAIT YSPVCLNSDA SGCNKVLYAS LQDSHLPPED
LYQLCHFNNG EICFPFQQGP LSTDDDGRLF SFDSMTSLTV SSSNYCSLSL KSCEKDGDDE
INDDFLAHCS PKLVIQQSID EIAPLKESTD LLDISNFTPD KFRHSSLLEM SPPDTPSLSP
QSTRCESIKT LGTMKGFQEG VPGSLSTVEK IKWDCNTLSQ QAQADDGFTL NSHQFQFHMF
NDEDSVGLLQ KSPCLSTFDE PAGQINTNSK VSKSRKKTSP GKSGAVSQSS SQKNSRKKSP
KASNKGVEKP PSKTSRQVPK STKKGKYVAA VNGEKMQIGI GHSGGQPNST SSNAKTLTEC
IQHGGPVASM KIPSQKGLSG DWALGKESRP GWNDMSVVTN TNNLLDDDQR EFQEPSYILS
NIASGMADVQ RFMMASMEPL WEPMEHQGES NTFYSPDSNS LKLKTLKILA GTPQESKKKV
TNGSSGATKN HRSVKAVSKS NGKAAIGEPG HADMPGSSED SRSAFFDKKY SNVNTLGNNG
PTHKKLYRHK SSSKGLRDEK YKGKRVEREQ AHKDEAGTTS FEKLRDSNYN LLKAETAFGV
LPVFEEETHI FQKDI
